ID   SPI7_SOLTU              Reviewed;         221 AA.
AC   P30941; Q41481; Q41482;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Serine protease inhibitor 7;
DE   AltName: Full=PIGEN1;
DE            Short=PIG;
DE   AltName: Full=STPIA;
DE   AltName: Full=STPIB;
DE   AltName: Full=pF4;
DE   AltName: Full=pKEN14-28;
DE   AltName: Allergen=Sola t 4;
DE   Flags: Precursor;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Pentland squire; TISSUE=Callus;
RX   PubMed=1391774; DOI=10.1007/bf00014499;
RA   Barlic-Maganja D., Strukelj B., Pungercar J., Gubensek F., Turk V.,
RA   Kregar I.;
RT   "Isolation and sequence analysis of the genomic DNA fragment encoding an
RT   aspartic proteinase inhibitor homologue from potato (Solanum tuberosum
RT   L.).";
RL   Plant Mol. Biol. 20:311-313(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Pentland squire; TISSUE=Tuber;
RX   PubMed=1515078; DOI=10.1515/bchm3.1992.373.2.477;
RA   Strukelj B., Pungercar J., Mesko P., Barlic-Maganja D., Gubensek F.,
RA   Kregar I., Turk V.;
RT   "Characterization of aspartic proteinase inhibitors from potato at the
RT   gene, cDNA and protein levels.";
RL   Biol. Chem. Hoppe-Seyler 373:477-482(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 29-38.
RC   STRAIN=cv. Danshaku;
RX   PubMed=8069493;
RA   Ishikawa A., Ohta S., Matsuoka K., Hattori T., Nakamura K.;
RT   "A family of potato genes that encode Kunitz-type proteinase inhibitors:
RT   structural comparisons and differential expression.";
RL   Plant Cell Physiol. 35:303-312(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. DM1-3 516 R44;
RX   PubMed=21743474; DOI=10.1038/nature10158;
RG   The Potato Genome Sequencing Consortium;
RT   "Genome sequence and analysis of the tuber crop potato.";
RL   Nature 475:189-195(2011).
CC   -!- FUNCTION: Inhibitor of trypsin (serine protease). May protect the plant
CC       by inhibiting proteases of invading organisms (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Vacuole.
CC   -!- TISSUE SPECIFICITY: Tubers. Not detected in root, stem, leaves or
CC       flower bud.
CC   -!- INDUCTION: Not induced by sucrose, methyl jasmonate or wounding.
CC   -!- ALLERGEN: Causes an allergic reaction in human.
CC   -!- MISCELLANEOUS: Has a single chain structure.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
CC   -!- CAUTION: Was originally (PubMed:1391774 and PubMed:1515078) thought to
CC       be an aspartic proteinase inhibitor. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X64370; CAA45723.1; -; Genomic_DNA.
DR   EMBL; D17329; BAA04149.1; -; mRNA.
DR   EMBL; D17330; BAA04150.1; -; mRNA.
DR   PIR; S25314; S25314.
DR   AlphaFoldDB; P30941; -.
DR   SMR; P30941; -.
DR   STRING; 4113.PGSC0003DMT400026257; -.
DR   Allergome; 3492; Sola t 4.0101.
DR   Allergome; 642; Sola t 4.
DR   MEROPS; I03.020; -.
DR   PRIDE; P30941; -.
DR   EnsemblPlants; PGSC0003DMT400026257; PGSC0003DMT400026257; PGSC0003DMG400010128.
DR   Gramene; PGSC0003DMT400026257; PGSC0003DMT400026257; PGSC0003DMG400010128.
DR   HOGENOM; CLU_090145_2_0_1; -.
DR   OMA; LHINQDI; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; P30941; baseline.
DR   GO; GO:0005773; C:vacuole; IDA:UniProtKB.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Reference proteome; Serine protease inhibitor; Signal; Vacuole.
FT   SIGNAL          1..22
FT   PROPEP          23..28
FT                   /evidence="ECO:0000269|PubMed:8069493"
FT                   /id="PRO_0000016908"
FT   CHAIN           29..221
FT                   /note="Serine protease inhibitor 7"
FT                   /id="PRO_0000016909"
FT   MOTIF           25..30
FT                   /note="Vacuolar targeting signal"
FT                   /evidence="ECO:0000250"
FT   SITE            95..96
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250"
FT   SITE            143..144
FT                   /note="Reactive bond for chymotrypsin"
FT                   /evidence="ECO:0000250"
FT   DISULFID        76..125
FT                   /evidence="ECO:0000250"
FT   DISULFID        174..191
FT                   /evidence="ECO:0000250"
FT   CONFLICT        97..100
FT                   /note="Missing (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        181
FT                   /note="S -> R (in Ref. 3; BAA04150)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   221 AA;  24028 MW;  67BE752A42795D2D CRC64;
     MKCLFLLCLC LVPIVVFSST FTSKNPINLP SDATPVLDVA GKELDSRLSY RIISTFWGAL
     GGDVYLGKSP NSDAPCANGI FRYNSDVGPS GTPVRFIGSS SHFGQGIFEN ELLNIQFAIS
     TSKLCVSYTI WKVGDYDASL GTMLLETGGT IGQADSSWFK IVKSSQFGYN LLYCPVTSTM
     SCPFSSDDQF CLKVGVVHQN GKRRLALVKD NPLDVSFKQV Q