ID   SPIB_RAT                Reviewed;         269 AA.
AC   Q5EBA3;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Transcription factor Spi-B;
GN   Name=Spib;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Sequence specific transcriptional activator which binds to
CC       the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a
CC       lymphoid-specific enhancer. Promotes development of plasmacytoid
CC       dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or
CC       natural interferon (IFN)-producing cells. These cells have the capacity
CC       to produce large amounts of interferon and block viral replication.
CC       Required for B-cell receptor (BCR) signaling, which is necessary for
CC       normal B-cell development and antigenic stimulation (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Can form homotypic interactions. Interacts with IRF4. May also
CC       interact with CREBBP, EP300, SPI1/SPI-1/PU.1 related, JUN and TBP (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q01892}.
CC   -!- DOMAIN: The protein contains a weakly acidic N-terminal transactivation
CC       domain (TAD) followed by a second TAD rich in proline, serine and
CC       threonine. Each of these domains may be required for transcriptional
CC       activation of a subset of target genes (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC089881; AAH89881.1; -; mRNA.
DR   RefSeq; NP_001019457.1; NM_001024286.1.
DR   AlphaFoldDB; Q5EBA3; -.
DR   SMR; Q5EBA3; -.
DR   STRING; 10116.ENSRNOP00000026599; -.
DR   PaxDb; Q5EBA3; -.
DR   GeneID; 499146; -.
DR   KEGG; rno:499146; -.
DR   UCSC; RGD:1565899; rat.
DR   CTD; 6689; -.
DR   RGD; 1565899; Spib.
DR   eggNOG; KOG3805; Eukaryota.
DR   HOGENOM; CLU_066451_2_0_1; -.
DR   InParanoid; Q5EBA3; -.
DR   OrthoDB; 1272250at2759; -.
DR   PhylomeDB; Q5EBA3; -.
DR   PRO; PR:Q5EBA3; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:RGD.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:0002327; P:immature B cell differentiation; ISO:RGD.
DR   GO; GO:0030225; P:macrophage differentiation; ISO:RGD.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:RGD.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000418; Ets_dom.
DR   InterPro; IPR046328; ETS_fam.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR11849; PTHR11849; 1.
DR   Pfam; PF00178; Ets; 1.
DR   PRINTS; PR00454; ETSDOMAIN.
DR   SMART; SM00413; ETS; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR   PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR   PROSITE; PS50061; ETS_DOMAIN_3; 1.
PE   2: Evidence at transcript level;
KW   Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..269
FT                   /note="Transcription factor Spi-B"
FT                   /id="PRO_0000204138"
FT   DNA_BIND        176..259
FT                   /note="ETS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT   REGION          1..32
FT                   /note="TAD1 (Acidic)"
FT   REGION          42..63
FT                   /note="TAD2"
SQ   SEQUENCE   269 AA;  29400 MW;  B29710EFFEF069B8 CRC64;
     MLALEAAQLD GPHLSCLQYP EGVFYDLDSC KSFSYPDSDG GPDSTWGWTE APPAPAIAAY
     EAFDPAATAA FAHTQAVQLC YGHGPSPSTY SPVGTLDPAP SLEASGPGLQ VYPSEDFTSQ
     TLGSLAYAPY PSPVLSEEED ILLDSPALEV SDSESDEALL AGSEGRGSEA GARKKLRLYQ
     FLLELLLRGD MRECVWWVEP GAGVFQFSSK HKELLARRWG QQKGNRKRMT YQKLARALRN
     YAKTGEIRKV KRKLTYQFDS ALLPAARRA