SPICE_MOUSE
ID SPICE_MOUSE Reviewed; 860 AA.
AC Q8C804; Q8K3I7;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Spindle and centriole-associated protein 1;
DE AltName: Full=Coiled-coil domain-containing protein 52;
GN Name=Spice1; Synonyms=Ccdc52, D16Ertd480e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=BALB/cJ; TISSUE=Brain;
RA Guo J.H.;
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-236; SER-240; SER-765;
RP SER-766; SER-769 AND SER-824, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP INTERACTION WITH CEP120.
RX PubMed=20360068; DOI=10.1126/science.1181348;
RA Hutchins J.R., Toyoda Y., Hegemann B., Poser I., Heriche J.K., Sykora M.M.,
RA Augsburg M., Hudecz O., Buschhorn B.A., Bulkescher J., Conrad C.,
RA Comartin D., Schleiffer A., Sarov M., Pozniakovsky A., Slabicki M.M.,
RA Schloissnig S., Steinmacher I., Leuschner M., Ssykor A., Lawo S.,
RA Pelletier L., Stark H., Nasmyth K., Ellenberg J., Durbin R., Buchholz F.,
RA Mechtler K., Hyman A.A., Peters J.M.;
RT "Systematic analysis of human protein complexes identifies chromosome
RT segregation proteins.";
RL Science 328:593-599(2010).
CC -!- FUNCTION: Regulator required for centriole duplication, for proper
CC bipolar spindle formation and chromosome congression in mitosis.
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CEP120. {ECO:0000269|PubMed:20360068}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriole {ECO:0000250}. Cytoplasm, cytoskeleton,
CC spindle {ECO:0000250}.
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DR EMBL; AK048789; BAC33458.1; -; mRNA.
DR EMBL; AY099108; AAM34496.1; -; mRNA.
DR EMBL; BC052381; AAH52381.1; -; mRNA.
DR EMBL; BC056960; AAH56960.1; -; mRNA.
DR CCDS; CCDS37345.1; -.
DR RefSeq; NP_653133.3; NM_144550.4.
DR RefSeq; XP_017172412.1; XM_017316923.1.
DR AlphaFoldDB; Q8C804; -.
DR SMR; Q8C804; -.
DR BioGRID; 229332; 3.
DR IntAct; Q8C804; 3.
DR STRING; 10090.ENSMUSP00000058832; -.
DR iPTMnet; Q8C804; -.
DR PhosphoSitePlus; Q8C804; -.
DR EPD; Q8C804; -.
DR MaxQB; Q8C804; -.
DR PaxDb; Q8C804; -.
DR PeptideAtlas; Q8C804; -.
DR PRIDE; Q8C804; -.
DR ProteomicsDB; 257350; -.
DR DNASU; 212514; -.
DR Ensembl; ENSMUST00000050897; ENSMUSP00000058832; ENSMUSG00000043065.
DR GeneID; 212514; -.
DR KEGG; mmu:212514; -.
DR UCSC; uc007zhg.2; mouse.
DR CTD; 152185; -.
DR MGI; MGI:1196252; Spice1.
DR VEuPathDB; HostDB:ENSMUSG00000043065; -.
DR eggNOG; ENOG502QQ0H; Eukaryota.
DR GeneTree; ENSGT00390000006207; -.
DR HOGENOM; CLU_016571_0_0_1; -.
DR InParanoid; Q8C804; -.
DR OMA; RHEMHKS; -.
DR OrthoDB; 351032at2759; -.
DR PhylomeDB; Q8C804; -.
DR TreeFam; TF333301; -.
DR BioGRID-ORCS; 212514; 4 hits in 71 CRISPR screens.
DR PRO; PR:Q8C804; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8C804; protein.
DR Bgee; ENSMUSG00000043065; Expressed in spermatocyte and 269 other tissues.
DR Genevisible; Q8C804; MM.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005819; C:spindle; ISS:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051310; P:metaphase plate congression; ISO:MGI.
DR GO; GO:0090307; P:mitotic spindle assembly; ISS:UniProtKB.
DR GO; GO:0046599; P:regulation of centriole replication; ISS:UniProtKB.
DR InterPro; IPR031387; SPICE1.
DR PANTHER; PTHR31167; PTHR31167; 1.
DR Pfam; PF15678; SPICE; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton; Mitosis;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..860
FT /note="Spindle and centriole-associated protein 1"
FT /id="PRO_0000282414"
FT REGION 127..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 172..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 623..645
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 693..718
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 792..860
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 383..439
FT /evidence="ECO:0000255"
FT COILED 729..757
FT /evidence="ECO:0000255"
FT COMPBIAS 135..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..332
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 795..839
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 236
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 240
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 648
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N0Z3"
FT MOD_RES 765
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 766
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 769
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 824
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 482
FT /note="E -> G (in Ref. 1; BAC33458)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 860 AA; 95645 MW; DC919B72DCE5D621 CRC64;
MSLIRVNRFG PRGGGRKTLK VKKKAAVRQE WDNTVNDLTV HRATPEDLVR RHEMHKSKNR
ALVHWELQEK ALKRKWKKQK PETSSLEKRK LSIMKEILSD QYLTQDVLEK SDHLMAAAKG
LFADVPRKRT GFPNVTRAPD SSQSHTGINQ DPVTQSILNE SIIEPQALNE VDDAGEQSTA
HSQSEDSENE LPNSLSQHSN RSTERFLHQL KEENSELINQ LWTDIQQKIA TQSQRTPPGS
PSSELSAEDQ KAALNATDAV KRIQAGPQPE EAAEPVDFSS SYLGQVLNTR KQKPLLAKVK
RKQDMHAASK QKTNMLSSST ASADRPSSTG SSLDVLKHVI HEVEHEMEEY ERCTGREVTG
LQGGQGLTGF TLSLVSSLCR LVRYLKESEI QLRKEVETRQ QLEQMLGDHR ELIDALTAEI
LSLREENSTM QARLQQYMVT TDEQLISLTH AIKNCPVINN SRQESQAPER AAMGRRLVDN
VEGPVISSNG SMPLMFRGEE VVEFPQEELP VKLSQGPTPT ENLNLANNFP THIFEPAVML
TPPRQKSNSE FSPLQDVLRR TVQTRPAPRI PPTVEVIEKE QNWEKKALPI DPDIQNSSEE
NRLFTQRWRV SHMGEDLENK GQPAFVSLSQ PPCSSLPSTQ QPRNPVLSEE PTVLGDGQQL
RTSEALVQRK DIMARIAELT LQNSAIKAHL NNITSSGGEQ GDGLREPRKQ GSASEVSTNF
PAVQSLTPSS MEERIAELNR QSMEARSKLL QLIEQQKLVG LNLSSSPVSP VESPLRAWAE
EGKRTIEVSV PGMEASESSK CNTVSPVSGN SSRRSSGAIS NSCSPLNATS GSGKFTPVNP
RTKTEKKNEE GWFALSAHIP
