SPIC_MOUSE
ID SPIC_MOUSE Reviewed; 242 AA.
AC Q6P3D7; Q3U064; Q3U0G6; Q3U1E9; Q9Z0Y0;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Transcription factor Spi-C;
DE AltName: Full=Pu.1-related factor;
DE Short=Prf;
GN Name=Spic;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DNA-BINDING, AND TISSUE
RP SPECIFICITY.
RX PubMed=10187812; DOI=10.1074/jbc.274.15.10259;
RA Bemark M., Martensson A., Liberg D., Leanderson T.;
RT "Spi-C, a novel Ets protein that is temporally regulated during B
RT lymphocyte development.";
RL J. Biol. Chem. 274:10259-10267(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Bone, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP DNA-BINDING, AND TISSUE SPECIFICITY.
RX PubMed=10464163; DOI=10.1093/intimm/11.9.1423;
RA Hashimoto S., Nishizumi H., Hayashi R., Tsuboi A., Nagawa F., Takemori T.,
RA Sakano H.;
RT "Prf, a novel Ets family protein that binds to the PU.1 binding motif, is
RT specifically expressed in restricted stages of B cell development.";
RL Int. Immunol. 11:1423-1429(1999).
RN [6]
RP DISRUPTION PHENOTYPE, FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19037245; DOI=10.1038/nature07472;
RA Kohyama M., Ise W., Edelson B.T., Wilker P.R., Hildner K., Mejia C.,
RA Frazier W.A., Murphy T.L., Murphy K.M.;
RT "Role for Spi-C in the development of red pulp macrophages and splenic iron
RT homeostasis.";
RL Nature 457:318-321(2009).
CC -!- FUNCTION: Controls the development of red pulp macrophages required for
CC red blood cells recycling and iron homeostasis. Transcription factor
CC that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3')
CC that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene
CC expression. {ECO:0000269|PubMed:19037245}.
CC -!- SUBUNIT: Binds DNA as a monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6P3D7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P3D7-2; Sequence=VSP_037734, VSP_037735;
CC -!- TISSUE SPECIFICITY: Expressed in lymphoid tissues, including spleen,
CC bone marrow and thymus. According to PubMed:19037245, highly expressed
CC in red pulp macrophages and, at lower, levels in B-cells, but not in
CC other cells, including, monocytes, dendritic cells and other tissue
CC macrophages. According to PubMed:10464163 expressed in pre- and mature
CC B-cells but not in immature B-cells; according to PubMed:10187812 not
CC expressed in pre- but predominantly in mature B-cells and at lower
CC levels in macrophages. {ECO:0000269|PubMed:10187812,
CC ECO:0000269|PubMed:10464163, ECO:0000269|PubMed:19037245}.
CC -!- DISRUPTION PHENOTYPE: Cell-autonomous defect in the development of red
CC pulp macrophages, but normal monocyte and other macrophage subsets.
CC Mice show normal trapping of red blood cells in the spleen, but fail to
CC phagocytose these cells efficiently and develop an iron overload
CC localized selectively to splenic red pulp.
CC {ECO:0000269|PubMed:19037245}.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; AF098863; AAD16228.2; -; mRNA.
DR EMBL; AK137522; BAE23394.1; -; mRNA.
DR EMBL; AK156024; BAE33549.1; -; mRNA.
DR EMBL; AK156892; BAE33887.1; -; mRNA.
DR EMBL; AK157188; BAE33991.1; -; mRNA.
DR EMBL; CH466539; EDL21490.1; -; Genomic_DNA.
DR EMBL; BC064038; AAH64038.1; -; mRNA.
DR CCDS; CCDS36023.1; -. [Q6P3D7-1]
DR RefSeq; NP_035591.3; NM_011461.3. [Q6P3D7-1]
DR AlphaFoldDB; Q6P3D7; -.
DR SMR; Q6P3D7; -.
DR STRING; 10090.ENSMUSP00000004473; -.
DR iPTMnet; Q6P3D7; -.
DR PhosphoSitePlus; Q6P3D7; -.
DR PaxDb; Q6P3D7; -.
DR PRIDE; Q6P3D7; -.
DR ProteomicsDB; 257351; -. [Q6P3D7-1]
DR ProteomicsDB; 257352; -. [Q6P3D7-2]
DR Antibodypedia; 30388; 143 antibodies from 23 providers.
DR DNASU; 20728; -.
DR Ensembl; ENSMUST00000004473; ENSMUSP00000004473; ENSMUSG00000004359. [Q6P3D7-1]
DR GeneID; 20728; -.
DR KEGG; mmu:20728; -.
DR UCSC; uc007grv.2; mouse. [Q6P3D7-1]
DR CTD; 121599; -.
DR MGI; MGI:1341168; Spic.
DR VEuPathDB; HostDB:ENSMUSG00000004359; -.
DR eggNOG; KOG3805; Eukaryota.
DR GeneTree; ENSGT00940000155067; -.
DR HOGENOM; CLU_098156_0_0_1; -.
DR InParanoid; Q6P3D7; -.
DR OMA; EIFYSPY; -.
DR OrthoDB; 1272250at2759; -.
DR PhylomeDB; Q6P3D7; -.
DR TreeFam; TF318679; -.
DR BioGRID-ORCS; 20728; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q6P3D7; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q6P3D7; protein.
DR Bgee; ENSMUSG00000004359; Expressed in morula and 83 other tissues.
DR ExpressionAtlas; Q6P3D7; baseline and differential.
DR Genevisible; Q6P3D7; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IMP:NTNU_SB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IMP:NTNU_SB.
DR GO; GO:0001824; P:blastocyst development; IMP:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 1.
DR Pfam; PF00178; Ets; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..242
FT /note="Transcription factor Spi-C"
FT /id="PRO_0000204141"
FT DNA_BIND 112..195
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT VAR_SEQ 1..101
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_037734"
FT VAR_SEQ 102..107
FT /note="QQKGGR -> MLLMNN (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_037735"
FT CONFLICT 80
FT /note="E -> G (in Ref. 1; AAD16228)"
FT /evidence="ECO:0000305"
FT CONFLICT 96
FT /note="V -> A (in Ref. 2; BAE33549)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 242 AA; 27942 MW; F4F77B59CC0F7F3C CRC64;
MTCCIDQDSL GQTFQDAIDI LIQQSAGESQ YSSENRNYMA IINPYPHVRG NANYYGMSPT
ENPLYDWRGV TNGSADLYLE GGFHQSVQNI AESQLVQPPF FQQKGGRGRR KLRLFEYLFE
SLCNSEMVSC IQWVDKARGI FQFISKNKET LAELWGQRKG NRKPMTYQKM ARALRNYART
GEIIKIRRKL TYQFSEAVLQ RLAPANYLGK DLFYPQYGQP DQGYLSLNHW NANHYAHGSY
QS
