SPIC_SALT1
ID SPIC_SALT1 Reviewed; 133 AA.
AC D0ZWR8; P74863;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 19-JAN-2010, sequence version 1.
DT 03-AUG-2022, entry version 42.
DE RecName: Full=Salmonella pathogenicity island 2 protein C;
DE AltName: Full=Secretion system apparatus protein B;
GN Name=spiC; Synonyms=ssaB; OrderedLocusNames=STM14_1688;
OS Salmonella typhimurium (strain 14028s / SGSC 2262).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=588858;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=14028s / SGSC 2262;
RX PubMed=8755556; DOI=10.1073/pnas.93.15.7800;
RA Ochman H., Soncini F.C., Solomon F., Groisman E.A.;
RT "Identification of a pathogenicity island required for Salmonella survival
RT in host cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:7800-7804(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=14028s / SGSC 2262;
RX PubMed=19897643; DOI=10.1128/jb.01233-09;
RA Jarvik T., Smillie C., Groisman E.A., Ochman H.;
RT "Short-term signatures of evolutionary change in the Salmonella enterica
RT serovar typhimurium 14028 genome.";
RL J. Bacteriol. 192:560-567(2010).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION, SECRETION VIA SPI2 TYPE III
RP SECRETION SYSTEM, AND DISRUPTION PHENOTYPE.
RC STRAIN=14028s / SGSC 2262;
RX PubMed=10406797; DOI=10.1093/emboj/18.14.3924;
RA Uchiya K., Barbieri M.A., Funato K., Shah A.H., Stahl P.D., Groisman E.A.;
RT "A Salmonella virulence protein that inhibits cellular trafficking.";
RL EMBO J. 18:3924-3933(1999).
RN [4]
RP INTERACTION WITH NIPSNAP3A.
RC STRAIN=14028s / SGSC 2262;
RX PubMed=12427096; DOI=10.1046/j.1462-5822.2002.00225.x;
RA Lee A.H., Zareei M.P., Daefler S.;
RT "Identification of a NIPSNAP homologue as host cell target for Salmonella
RT virulence protein SpiC.";
RL Cell. Microbiol. 4:739-750(2002).
RN [5]
RP INTERACTION WITH HOST HOOK3.
RC STRAIN=14028s / SGSC 2262;
RX PubMed=12950921; DOI=10.1046/j.1365-2958.2003.03668.x;
RA Shotland Y., Kraemer H., Groisman E.A.;
RT "The Salmonella SpiC protein targets the mammalian Hook3 protein function
RT to alter cellular trafficking.";
RL Mol. Microbiol. 49:1565-1576(2003).
RN [6]
RP FUNCTION IN SPVB ACTIVITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=14028s / SGSC 2262;
RX PubMed=18248436; DOI=10.1111/j.1574-695x.2007.00364.x;
RA Browne S.H., Hasegawa P., Okamoto S., Fierer J., Guiney D.G.;
RT "Identification of Salmonella SPI-2 secretion system components required
RT for SpvB-mediated cytotoxicity in macrophages and virulence in mice.";
RL FEMS Immunol. Med. Microbiol. 52:194-201(2008).
CC -!- FUNCTION: Virulence protein that plays a central role in mammalian
CC macrophage infection, by inhibiting phagosome-lysosome fusion and
CC cellular trafficking, including trafficking of organelles that are
CC devoid of Salmonella. May act by disrupting the function of the
CC mammalian HOOK3 protein, a protein involved in the cellular traffic.
CC Also required for actin ADP-ribosylase SpvB activity.
CC {ECO:0000269|PubMed:10406797, ECO:0000269|PubMed:18248436}.
CC -!- SUBUNIT: Interacts with the mammalian NIPSNAP3A and HOOK3 proteins in
CC infected cells. {ECO:0000269|PubMed:12427096,
CC ECO:0000269|PubMed:12950921}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10406797}. Cytoplasm
CC {ECO:0000269|PubMed:10406797}. Note=Translocated into the cytosol of
CC macrophages via the Salmonella pathogenicity island 2 (SPI-2) type III
CC secretion system that functions intracellularly to translocate proteins
CC across the phagosomal membrane. Cytoplasmic in infected macrophages.
CC -!- INDUCTION: Protein levels in intracellular macrophage host increase
CC with infection time (at protein level). {ECO:0000269|PubMed:10406797}.
CC -!- DISRUPTION PHENOTYPE: Does not survive in murine macrophages, although
CC it can invade epithelial cells. Disruption restores host cell
CC phagosome-lysosome fusion and host vesicular trafficking. Also prevents
CC SpvB-induced F-actin depolymerization in human macrophages without
CC affecting intra-bacterial SpvB protein levels.
CC {ECO:0000269|PubMed:10406797, ECO:0000269|PubMed:18248436}.
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DR EMBL; U51927; AAC44300.1; -; Genomic_DNA.
DR EMBL; CP001363; ACY88166.1; -; Genomic_DNA.
DR RefSeq; WP_001738217.1; NZ_CP043402.1.
DR AlphaFoldDB; D0ZWR8; -.
DR EnsemblBacteria; ACY88166; ACY88166; STM14_1688.
DR KEGG; seo:STM14_1688; -.
DR PATRIC; fig|588858.6.peg.1623; -.
DR HOGENOM; CLU_157272_0_0_6; -.
DR OMA; HPDIHDY; -.
DR BioCyc; SENT588858:STM14_RS07820-MON; -.
DR PHI-base; PHI:2626; -.
DR PHI-base; PHI:3759; -.
DR Proteomes; UP000002695; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cytoplasm; Protein transport; Secreted; Transport; Virulence.
FT CHAIN 1..133
FT /note="Salmonella pathogenicity island 2 protein C"
FT /id="PRO_0000410496"
SQ SEQUENCE 133 AA; 14656 MW; 950693E4F8D909D0 CRC64;
MSEEGFMLAV LKGIPLIQDI RAEGNSRSWI MTIDGHPARG EIFSEAFSIS LFLNDLESLP
KPCLAYVTLL LAAHPDVHDY AIQLTADGGW LNGYYTTSSS SELIAIEIEK HLALTCILKN
VIRNHHKLYS GGV
