ID   SPIC_SALTY              Reviewed;         133 AA.
AC   P0CZ04; P74863;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=Salmonella pathogenicity island 2 protein C;
DE   AltName: Full=Secretion system apparatus protein B;
GN   Name=spiC; Synonyms=ssaB; OrderedLocusNames=STM1393;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 14028 / SGSC 2980 / CDC 6516-60 / NCTC 12023;
RX   PubMed=12174087; DOI=10.1046/j.1462-5822.2002.00211.x;
RA   Yu X.-J., Ruiz-Albert J., Unsworth K.E., Garvis S., Liu M., Holden D.W.;
RT   "SpiC is required for secretion of Salmonella pathogenicity island 2 type
RT   III secretion system proteins.";
RL   Cell. Microbiol. 4:531-540(2002).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 14028 / SGSC 2980 / CDC 6516-60 / NCTC 12023;
RX   PubMed=12193612; DOI=10.1128/jb.184.18.4971-4980.2002;
RA   Freeman J.A., Rappl C., Kuhle V., Hensel M., Miller S.I.;
RT   "SpiC is required for translocation of Salmonella pathogenicity island 2
RT   effectors and secretion of translocon proteins SseB and SseC.";
RL   J. Bacteriol. 184:4971-4980(2002).
CC   -!- FUNCTION: Virulence protein that plays a central role in mammalian
CC       macrophage infection, by inhibiting phagosome-lysosome fusion and
CC       cellular trafficking. May act by disrupting the function of the
CC       mammalian HOOK3 protein, a protein involved in the cellular traffic.
CC       {ECO:0000269|PubMed:12174087, ECO:0000269|PubMed:12193612}.
CC   -!- SUBUNIT: Interacts with the mammalian NIPSNAP3A and HOOK3 proteins in
CC       infected cells. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted. Cytoplasm. Note=Translocated into the
CC       cytosol of macrophages via the Salmonella pathogenicity island 2 (SPI-
CC       2) type III secretion system that functions intracellularly to
CC       translocate proteins across the phagosomal membrane. Cytoplasmic in
CC       infected macrophages.
CC   -!- CAUTION: PubMed:12174087 and PubMed:12193612 report that SpiC is not
CC       translocated into the infected cell, and is localized in the bacterial
CC       cytoplasm. They show it is required for secretion of SseB and SseC
CC       virulence proteins into infected cells. {ECO:0000305}.
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DR   EMBL; AE006468; AAL20317.1; -; Genomic_DNA.
DR   RefSeq; NP_460358.1; NC_003197.2.
DR   RefSeq; WP_001738217.1; NC_003197.2.
DR   AlphaFoldDB; P0CZ04; -.
DR   STRING; 99287.STM1393; -.
DR   PaxDb; P0CZ04; -.
DR   EnsemblBacteria; AAL20317; AAL20317; STM1393.
DR   GeneID; 1252911; -.
DR   KEGG; stm:STM1393; -.
DR   PATRIC; fig|99287.12.peg.1477; -.
DR   HOGENOM; CLU_157272_0_0_6; -.
DR   OMA; HPDIHDY; -.
DR   BioCyc; SENT99287:STM1393-MON; -.
DR   PHI-base; PHI:555; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Cytoplasm; Protein transport; Reference proteome; Secreted; Transport;
KW   Virulence.
FT   CHAIN           1..133
FT                   /note="Salmonella pathogenicity island 2 protein C"
FT                   /id="PRO_0000072126"
SQ   SEQUENCE   133 AA;  14656 MW;  950693E4F8D909D0 CRC64;
     MSEEGFMLAV LKGIPLIQDI RAEGNSRSWI MTIDGHPARG EIFSEAFSIS LFLNDLESLP
     KPCLAYVTLL LAAHPDVHDY AIQLTADGGW LNGYYTTSSS SELIAIEIEK HLALTCILKN
     VIRNHHKLYS GGV