ID   SPIKE_ADEB3             Reviewed;         976 AA.
AC   Q03553;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Fiber protein;
DE            Short=SPIKE;
DE   AltName: Full=Protein IV;
GN   ORFNames=L5;
OS   Bovine adenovirus B serotype 3 (BAdV-3) (Mastadenovirus bos3).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Rowavirales; Adenoviridae; Mastadenovirus; Bovine mastadenovirus B.
OX   NCBI_TaxID=10510;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1469367; DOI=10.1099/0022-1317-73-12-3295;
RA   Mittal S.K., Prevec L., Babiuk L.A., Graham F.L.;
RT   "Sequence analysis of bovine adenovirus type 3 early region 3 and fibre
RT   protein genes.";
RL   J. Gen. Virol. 73:3295-3300(1992).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=8277294; DOI=10.1099/0022-1317-74-12-2825;
RA   Mittal S.K., Prevec L., Babiuk L.A., Graham F.L.;
RT   "Sequence analysis of bovine adenovirus type 3 early region 3 and fibre
RT   protein genes.";
RL   J. Gen. Virol. 74:2825-2825(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WBR-1;
RX   PubMed=9445040; DOI=10.1128/jvi.72.2.1394-1402.1998;
RA   Reddy P.S., Idamakanti N., Zakhartchouk A.N., Baxi M.K., Lee J.B., Pyne C.,
RA   Babiuk L.A., Tikoo S.K.;
RT   "Nucleotide sequence, genome organization, and transcription map of bovine
RT   adenovirus type 3.";
RL   J. Virol. 72:1394-1402(1998).
CC   -!- FUNCTION: Forms spikes that protrude from each vertex of the
CC       icosahedral capsid. Interacts with host receptor to provide virion
CC       initial attachment to target cell. Fiber proteins are shed during virus
CC       entry, when virus is still at the cell surface (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homotrimer. Interacts (via N-terminal tail region) with
CC       pentons (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host nucleus {ECO:0000250}.
CC       Note=Anchored to the pentons, protrudes from the virion surface.
CC       {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- DOMAIN: The tail region anchors the fiber to penton base capsomers,
CC       whereas the shaft, built from several repeated motifs, allows the knob
CC       to protrude from the virion. {ECO:0000250}.
CC   -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC       are produced by alternative splicing and alternative polyadenylation of
CC       the same gene giving rise to non-overlapping ORFs. A leader sequence is
CC       present in the N-terminus of all these mRNAs and is recognized by the
CC       viral shutoff protein to provide expression although conventional
CC       translation via ribosome scanning from the cap has been shut off in the
CC       host cell (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the adenoviridae fiber family. {ECO:0000305}.
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DR   EMBL; D16839; BAA04115.1; -; Genomic_DNA.
DR   EMBL; AF030154; AAD09736.1; -; Genomic_DNA.
DR   RefSeq; NP_046331.1; NC_001876.1.
DR   SMR; Q03553; -.
DR   GeneID; 2652975; -.
DR   KEGG; vg:2652975; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0098671; P:adhesion receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.90.10; -; 1.
DR   InterPro; IPR000931; Adeno_fibre.
DR   InterPro; IPR000939; Adenobir_fibre_prot_rpt/shaft.
DR   InterPro; IPR008982; Adenovirus_pIV-like_att.
DR   InterPro; IPR009013; Attachment_protein_shaft_sf.
DR   InterPro; IPR006626; PbH1.
DR   Pfam; PF00608; Adeno_shaft; 12.
DR   PRINTS; PR00307; ADENOVSFIBRE.
DR   SMART; SM00710; PbH1; 6.
DR   SUPFAM; SSF49835; SSF49835; 1.
DR   SUPFAM; SSF51225; SSF51225; 7.
PE   2: Evidence at transcript level;
KW   Capsid protein; Host nucleus; Host-virus interaction; Late protein;
KW   Viral attachment to host adhesion receptor; Viral attachment to host cell;
KW   Virion; Virus entry into host cell.
FT   CHAIN           1..976
FT                   /note="Fiber protein"
FT                   /id="PRO_0000221803"
SQ   SEQUENCE   976 AA;  102324 MW;  9D0EC36052F02896 CRC64;
     MKRSVPQDFN LVYPYKAKRP NIMPPFFDRN GFVENQEATL AMLVEKPLTF DKEGALTLGV
     GRGIRINPAG LLETNDLASA VFPPLASDEA GNVTLNMSDG LYTKDNKLAV KVGPGLSLDS
     NNALQVHTGD GLTVTDDKVS LNTQAPLSTT SAGLSLLLGP SLHLGEEERL TVNTGAGLQI
     SNNALAVKVG SGITVDAQNQ LAASLGDGLE SRDNKTVVKA GPGLTITNQA LTVATGNGLQ
     VNPEGQLQLN ITAGQGLNFA NNSLAVELGS GLHFPPGQNQ VSLYPGDGID IRDNRVTVPA
     GPGLRMLNHQ LAVASGDGLE VHSDTLRLKL SHGLTFENGA VRAKLGPGLG TDDSGRSVVR
     TGRGLRVANG QVQIFSGRGT AIGTDSSLTL NIRAPLQFSG PALTASLQGS GPITYNSNNG
     TFGLSIGPGM WVDQNRLQVN PGAGLVFQGN NLVPNLADPL AISDSKISLS LGPGLTQASN
     ALTLSLGNGL EFSNQAVAIK AGRGLRFESS SQALESSLTV GNGLTLTDTV IRPNLGDGLE
     VRDNKIIVKL GANLRFENGA VTAGTVNPSA PEAPPTLTAE PPLRASNSHL QLSLSEGLVV
     HNNALALQLG DGMEVNQHGL TLRVGSGLQM RDGILTVTPS GTPIEPRLTA PLTQTENGIG
     LALGAGLELD ESALQVKVGP GMRLNPVEKY VTLLLGPGLS FGQPANRTNY DVRVSVEPPM
     VFGQRGQLTF LVGHGLHIQN SKLQLNLGQG LRTDPVTNQL EVPLGQGLEI ADESQVRVKL
     GDGLQFDSQA RITTAPNMVT ETLWTGTGSN ANVTWRGYTA PGSKLFLSLT RFSTGLVLGN
     MTIDSNASFG QYINAGHEQI ECFILLDNQG NLKEGSNLQG TWEVKNNPSA SKAAFLPSTA
     LYPILNESRG SLPGKNLVGM QAILGGGGTC TVIATLNGRR SNNYPAGQSI IFVWQEFNTI
     ARQPLNHSTL TFSYWT