ID   SPIKE_ADEP3             Reviewed;         448 AA.
AC   Q83457; Q9YTR2;
DT   11-JUL-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Fiber protein;
DE            Short=SPIKE;
DE   AltName: Full=Protein IV;
GN   ORFNames=L5;
OS   Porcine adenovirus A serotype 3 (PAdV-3) (Porcine adenovirus 3).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Rowavirales; Adenoviridae; Mastadenovirus; Porcine mastadenovirus A.
OX   NCBI_TaxID=35265;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=6618;
RX   PubMed=7625129; DOI=10.1016/0168-1702(94)00105-l;
RA   Reddy P.S., Nagy E., Derbyshire J.B.;
RT   "Sequence analysis of putative pVIII, E3 and fibre regions of porcine
RT   adenovirus type 3.";
RL   Virus Res. 36:97-106(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=6618;
RX   PubMed=9837805; DOI=10.1006/viro.1998.9418;
RA   Reddy P.S., Idamakanti N., Song J.Y., Lee J.B., Hyun B.H., Park J.H.,
RA   Cha S.H., Bae Y.T., Tikoo S.K., Babiuk L.A.;
RT   "Nucleotide sequence and transcription map of porcine adenovirus type 3.";
RL   Virology 251:414-426(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=6618 / IAF;
RA   Larocque D., Malenfant F., Massie B., Dea S.;
RT   "Porcine adenovirus serotype 3, complete genome.";
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Forms spikes that protrude from each vertex of the
CC       icosahedral capsid. Interacts with host receptor to provide virion
CC       initial attachment to target cell. Fiber proteins are shed during virus
CC       entry, when virus is still at the cell surface (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homotrimer. Interacts (via N-terminal tail region) with
CC       pentons (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host nucleus {ECO:0000250}.
CC       Note=Anchored to the pentons, protrudes from the virion surface.
CC       {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- DOMAIN: The tail region anchors the fiber to penton base capsomers,
CC       whereas the shaft, built from several repeated motifs, allows the knob
CC       to protrude from the virion. {ECO:0000250}.
CC   -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC       are produced by alternative splicing and alternative polyadenylation of
CC       the same gene giving rise to non-overlapping ORFs. A leader sequence is
CC       present in the N-terminus of all these mRNAs and is recognized by the
CC       viral shutoff protein to provide expression although conventional
CC       translation via ribosome scanning from the cap has been shut off in the
CC       host cell (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the adenoviridae fiber family. {ECO:0000305}.
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DR   EMBL; AF083132; AAC99449.1; -; Genomic_DNA.
DR   EMBL; AJ237815; CAB41035.1; -; Genomic_DNA.
DR   EMBL; AB026117; BAA76973.1; -; Genomic_DNA.
DR   RefSeq; YP_009220.1; AC_000189.1.
DR   SMR; Q83457; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0098671; P:adhesion receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.90.10; -; 1.
DR   InterPro; IPR000931; Adeno_fibre.
DR   InterPro; IPR000939; Adenobir_fibre_prot_rpt/shaft.
DR   InterPro; IPR008982; Adenovirus_pIV-like_att.
DR   InterPro; IPR009013; Attachment_protein_shaft_sf.
DR   Pfam; PF00608; Adeno_shaft; 5.
DR   PRINTS; PR00307; ADENOVSFIBRE.
DR   SUPFAM; SSF49835; SSF49835; 1.
DR   SUPFAM; SSF51225; SSF51225; 2.
PE   2: Evidence at transcript level;
KW   Capsid protein; Host nucleus; Host-virus interaction; Late protein;
KW   Viral attachment to host adhesion receptor; Viral attachment to host cell;
KW   Virion; Virus entry into host cell.
FT   CHAIN           1..448
FT                   /note="Fiber protein"
FT                   /id="PRO_0000221811"
SQ   SEQUENCE   448 AA;  47846 MW;  8B929F41D16A0FA3 CRC64;
     MGPKKQKREL PEDFDPVYPY DVPQLQINPP FVSGDGFNQS VDGVLSLHIA PPLVFDNTRA
     LTLAFGGGLQ LSGKQLVVAT EGSGLTTNPD GKLVLKVKSP ITLTAEGISL SLGPGLSNSE
     TGLSLQVTAP LQFQGNALTL PLAAGLQNTD GGMGVKLGSG LTTDNSQAVT VQVGNGLQLN
     GEGQLTVPAT APLVSGSAGI SFNYSSNDFV LDNDSLSLRP KAISVTPPLQ STEDTISLNY
     SNDFSVDNGA LTLAPTFKPY TLWTGASPTA NVILTNTTTP NGTFFLCLTR VGGLVLGSFA
     LKSSIDLTSM TKKVNFIFDG AGRLQSDSTY KGRFGFRSND SVIEPTAAGL SPAWLMPSTF
     IYPRNTSGSS LTSFVYINQT YVHVDIKVNT LSTNGYSLEF NFQNMSFSAP FSTSYGTFCY
     VPRRTTHRPR HGPFSLRERR HLFQLLQQ