SPIKE_BRV1
ID SPIKE_BRV1 Reviewed; 1583 AA.
AC O90304; Q3T8J0;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Spike glycoprotein;
DE Short=S glycoprotein;
DE AltName: Full=E2;
DE AltName: Full=Peplomer protein;
DE Flags: Precursor;
GN Name=S; Synonyms=P;
OS Breda virus 1 (BRV-1).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Tornidovirineae; Tobaniviridae; Torovirinae; Torovirus;
OC Renitovirus.
OX NCBI_TaxID=360393;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=9879765; DOI=10.1016/s0168-1702(98)00104-x;
RA Duckmanton L.M., Tellier R., Liu P., Petric M.;
RT "Bovine torovirus: sequencing of the structural genes and expression of the
RT nucleocapsid protein of Breda virus.";
RL Virus Res. 58:83-96(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=16137782; DOI=10.1016/j.virusres.2005.07.005;
RA Draker R., Roper R.L., Petric M., Tellier R.;
RT "The complete sequence of the bovine torovirus genome.";
RL Virus Res. 115:56-68(2006).
CC -!- FUNCTION: Mediates the binding of virions to the host cell receptor and
CC is involved in membrane fusion. {ECO:0000250}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the torovirinae spike protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAS17959.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF076621; AAD03840.1; -; Genomic_RNA.
DR EMBL; AY427798; AAS17959.1; ALT_FRAME; Genomic_RNA.
DR RefSeq; YP_337907.1; NC_007447.1.
DR GeneID; 3707766; -.
DR KEGG; vg:3707766; -.
DR Proteomes; UP000000355; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039663; P:membrane fusion involved in viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR InterPro; IPR031412; S_torovirinae.
DR Pfam; PF17072; Spike_torovirin; 1.
PE 3: Inferred from homology;
KW Fusion of virus membrane with host membrane; Glycoprotein;
KW Host-virus interaction; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix; Viral attachment to host cell;
KW Viral envelope protein; Viral penetration into host cytoplasm; Virion;
KW Virulence; Virus entry into host cell.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..1583
FT /note="Spike glycoprotein"
FT /id="PRO_0000283903"
FT TOPO_DOM 21..1553
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT TRANSMEM 1554..1574
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1575..1583
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 82
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 252
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 384
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 494
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 573
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 842
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 936
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 970
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1268
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1299
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1387
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1391
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1430
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1433
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1440
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1485
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1489
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1497
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 1517
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT VARIANT 9
FT /note="P -> A"
FT VARIANT 27
FT /note="S -> T"
FT VARIANT 59..60
FT /note="TT -> ID"
FT VARIANT 72
FT /note="S -> N"
FT VARIANT 111..113
FT /note="VST -> QSF"
FT VARIANT 136
FT /note="A -> V"
FT VARIANT 141..143
FT /note="ESR -> DSW"
FT VARIANT 158
FT /note="H -> Y"
FT VARIANT 169
FT /note="H -> R"
FT VARIANT 177
FT /note="R -> C"
FT VARIANT 182
FT /note="L -> S"
FT VARIANT 212
FT /note="V -> L"
FT VARIANT 219
FT /note="S -> P"
FT VARIANT 250..252
FT /note="SCN -> GCK"
FT VARIANT 267..294
FT /note="ASAIILRSQLIVALAQKLSRTVGVNKAV -> ITAVTLPPDLKVPVVQKVTK
FT RLGVTSPD"
FT VARIANT 298..299
FT /note="FL -> LI"
FT VARIANT 302..304
FT /note="PYH -> AYQ"
FT VARIANT 308..311
FT /note="LVNF -> QAAI"
FT VARIANT 320..326
FT /note="PLCKSLR -> ALSNSLY"
FT VARIANT 330..336
FT /note="ATYSALS -> LVLTDLC"
FT VARIANT 357..360
FT /note="CCLY -> AVFT"
FT VARIANT 368
FT /note="S -> N"
FT VARIANT 378
FT /note="N -> K"
FT VARIANT 383
FT /note="A -> T"
FT VARIANT 457
FT /note="G -> GD"
FT VARIANT 464..467
FT /note="NGVI -> KDVT"
FT VARIANT 475
FT /note="S -> P"
FT VARIANT 507..510
FT /note="SFVR -> QFGS"
FT VARIANT 530
FT /note="Q -> H"
FT VARIANT 536
FT /note="V -> L"
FT VARIANT 540
FT /note="G -> A"
FT VARIANT 550
FT /note="I -> V"
FT VARIANT 558..561
FT /note="AQSS -> TQTQD"
FT VARIANT 617
FT /note="I -> V"
FT VARIANT 643
FT /note="I -> S"
FT VARIANT 654
FT /note="I -> S"
FT VARIANT 683..684
FT /note="TT -> LA"
FT VARIANT 690
FT /note="A -> P"
FT VARIANT 713
FT /note="D -> G"
FT VARIANT 718..725
FT /note="LSTMVPSI -> TTMVAPTL"
FT VARIANT 753..754
FT /note="RN -> QT"
FT VARIANT 778
FT /note="D -> S"
FT VARIANT 787
FT /note="F -> S"
FT VARIANT 813
FT /note="S -> G"
FT VARIANT 849
FT /note="Y -> H"
FT VARIANT 853
FT /note="T -> I"
FT VARIANT 856
FT /note="T -> M"
FT VARIANT 861
FT /note="E -> D"
FT VARIANT 978
FT /note="T -> I"
FT VARIANT 1060..1061
FT /note="SA -> RT"
FT VARIANT 1067
FT /note="I -> T"
FT VARIANT 1071
FT /note="H -> R"
FT VARIANT 1135
FT /note="I -> T"
FT VARIANT 1147
FT /note="D -> N"
FT VARIANT 1153..1154
FT /note="AG -> TD"
FT VARIANT 1157
FT /note="T -> E"
FT VARIANT 1161
FT /note="E -> K"
FT VARIANT 1180..1182
FT /note="TSA -> QVT"
FT VARIANT 1192
FT /note="F -> L"
FT VARIANT 1257..1260
FT /note="FQIC -> SQMF"
FT VARIANT 1270
FT /note="Missing"
FT VARIANT 1278
FT /note="L -> F"
FT VARIANT 1378
FT /note="A -> S"
FT VARIANT 1495..1496
FT /note="EL -> GV"
SQ SEQUENCE 1583 AA; 177786 MW; 0529C1293B38849C CRC64;
MFLCFCTAPI LCLWINSGGA VVVSNESLVV CEPVSYPYSL QVLRSFSQRV NLRTKRAVTT
DAWSFAYQIS TSSLNVNGWY VNFTSPLGWS YPNGKLFGIV LGSDAMMRAS VSTFTYDVIS
YVGQRPNLDC QINDLANGGL ESRYSTVRVD NCGNYPCHGG GKPGCSIGHP YMANGVRTRV
LLTTQSPGIQ YEIYSGQDYA VYQITPYTQY TVTMPSGTSG YCQQTPLYVE CGSWTPYRVH
AYGCDKATQS CNYTISSDWV VAFKSKASAI ILRSQLIVAL AQKLSRTVGV NKAVYFWFLK
QPYHYLSLVN FSPNYALFSP LCKSLRQQSA TYSALSYGSP FFVAQECYNN ALYLPDCCLY
TLFSILFSWD YQVNYPVNNV LQANETFLQL PTTGYLGQTV SQGRMLNLFK DAIVFLDFYD
TKFYRTNDGP GGDIFAVVVK QVPVIAYSAF RIEQQTGYLA VKCNGVIQAT LAPHSSRVVL
LARHMSMWSI AAANSTTIYC PIYTLTSFVR LDISTSWYFH TLAQPSGPIQ QVSMPVLSTG
AAGVYMHPMI EHWVTLLAQS SVYQPSMFNM GVNKSVTLTT QLQAYAQVYT AWFLSILYTR
LPESRRLTLG AQLTPFIQAL LSFKQADIDA TDVDTVARYN VLILMWGRKY AAVIYNQLPE
WSYPLFKGGV GDSMWFRKKF LVTTKIHQTA SHFPFIAGYL DFLDYKYIPK YKDVACPLST
MVPSILQVYE TPQLFVIIVQ CVSTTYSWYP GLRNPHTIYR SYKLGTICVL VPYSSPTDVY
SSFGFFFQSA LTIPTVQTTD DILPGCVGFV QDSVFTPCHP SGCPVRNSYD NYIICPGSSA
SNYTLRNYYR TTTPVTNVPI EEVPLQLEIP TVSLTSYELK QSESVLLQDI EGGIVVDHNT
GSIWYPGGQA YDVSFYVSVI IRYAPPKLEL PSTLANFTSC LDYICFGNQQ CRGEAQTFCT
SMDYFEQVFN KSLTSLITAL QDLHYVLKLV LPETTLELTE DTRRRRRAVD EFSDTISLLS
ESFERFMSPA SQAYMANMMW WDEAFDGISL PQRTGSILSS APSLSSISSW HSYSSRTPLI
SNVKTPKTTF NVKLSMPKLP KASTLSTIGS VLSSGLSIAS LGLSIFSIIE DRRVIELTQQ
QIMALEDQIT ILAGYTTKNF EEIQSSLNTL GQQVQDFSQT SALSLQQLSN GFEQITQQLD
KSIYYVMAVQ QYATYMSSLV NQLNELSQAV YKTQDMYITC IHSLQSGVLS PNCITPFQIC
HLYQVAKNLS SGECQPILSE REISRFYSLP LVTDAMVHND TYWFSWSIPI TCSNILGSVY
KVQPGYIVNP HHPTSLQYDV PTHVVTSNAG ALIFDEHYCD RYNQVYLCTK SAFDLAEASY
LTMLYSNQTD NSSLTFHPAP RPDPCVYLSA SALYCYYSDE CHQCVIAVGN CTNRTVTYEN
YTYPIMDPQC RGFDQITISS PIAIGADFTA LPSRPPLPLH LSYVNVTFNV TLPNELNWTD
LVLDYSFKDK VYEISKNITQ LHEQILQVSN WASGWFQRLR DFLYGLIPAW ITWLTLGFSL
FSILISGVNI ILFFEMNGKV KKS
