SPIKL_MOUSE
ID SPIKL_MOUSE Reviewed; 90 AA.
AC Q8CEK3;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Serine protease inhibitor kazal-like protein, minor form {ECO:0000303|PubMed:18715980};
DE Short=SPINKL, minor form {ECO:0000303|PubMed:18715980};
DE Contains:
DE RecName: Full=Serine protease inhibitor kazal-like protein, major form {ECO:0000303|PubMed:18715980};
DE Short=SPINKL, major form {ECO:0000303|PubMed:18715980};
DE Flags: Precursor;
GN Name=Spinkl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:BAC25634.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAC25634.1};
RC TISSUE=Urinary bladder {ECO:0000312|EMBL:BAC25634.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 24-42 AND 46-82, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, AND GLYCOSYLATION AT ASN-38.
RC STRAIN=ICR {ECO:0000269|PubMed:18715980};
RC TISSUE=Seminal vesicle {ECO:0000269|PubMed:18715980};
RX PubMed=18715980; DOI=10.1530/rep-07-0375;
RA Lin M.-H., Lee R.K.-K., Hwu Y.-M., Lu C.-H., Chu S.-L., Chen Y.-J.,
RA Chang W.-C., Li S.-H.;
RT "SPINKL, a Kazal-type serine protease inhibitor-like protein purified from
RT mouse seminal vesicle fluid, is able to inhibit sperm capacitation.";
RL Reproduction 136:559-571(2008).
CC -!- FUNCTION: Does not function as an inhibitor of trypsin, chymotrypsin,
CC subtilisin or elastase. Binds sperm and enhances sperm motility. May
CC act as a decapacitation factor, suppresses BSA-stimulated sperm
CC capacitation and blocks sperm-oocyte interactions in vitro.
CC {ECO:0000269|PubMed:18715980}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18715980}.
CC -!- TISSUE SPECIFICITY: Luminal fluid and mucosal folds of the seminal
CC vesicles (at protein level). Not detected in brain, heart, lung, liver,
CC kidney, stomach, small intestine, muscle, skin, thymus, placenta or
CC bladder. {ECO:0000269|PubMed:18715980}.
CC -!- DEVELOPMENTAL STAGE: First appears at low levels in 3 week old mice.
CC Levels increase rapidly after 4 weeks, highest levels are reached in 8
CC week old mice. {ECO:0000269|PubMed:18715980}.
CC -!- INDUCTION: By testosterone. {ECO:0000269|PubMed:18715980}.
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DR EMBL; AK020535; BAC25634.1; -; mRNA.
DR CCDS; CCDS50273.1; -.
DR RefSeq; NP_898946.1; NM_183123.2.
DR RefSeq; XP_006526415.1; XM_006526352.3.
DR AlphaFoldDB; Q8CEK3; -.
DR SMR; Q8CEK3; -.
DR STRING; 10090.ENSMUSP00000063376; -.
DR GlyGen; Q8CEK3; 1 site.
DR iPTMnet; Q8CEK3; -.
DR PaxDb; Q8CEK3; -.
DR PeptideAtlas; Q8CEK3; -.
DR PRIDE; Q8CEK3; -.
DR ProteomicsDB; 261617; -.
DR DNASU; 77424; -.
DR Ensembl; ENSMUST00000066328; ENSMUSP00000063376; ENSMUSG00000053729.
DR GeneID; 77424; -.
DR KEGG; mmu:77424; -.
DR UCSC; uc008eut.1; mouse.
DR CTD; 77424; -.
DR MGI; MGI:1924674; Spinkl.
DR VEuPathDB; HostDB:ENSMUSG00000053729; -.
DR GeneTree; ENSGT00940000164814; -.
DR HOGENOM; CLU_169765_0_0_1; -.
DR InParanoid; Q8CEK3; -.
DR OrthoDB; 1636538at2759; -.
DR PhylomeDB; Q8CEK3; -.
DR BioGRID-ORCS; 77424; 1 hit in 32 CRISPR screens.
DR ChiTaRS; Spinkl; mouse.
DR PRO; PR:Q8CEK3; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q8CEK3; protein.
DR Bgee; ENSMUSG00000053729; Expressed in seminal vesicle and 17 other tissues.
DR Genevisible; Q8CEK3; MM.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:1902491; P:negative regulation of sperm capacitation; IDA:MGI.
DR GO; GO:1902093; P:positive regulation of flagellated sperm motility; IDA:MGI.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR Pfam; PF00050; Kazal_1; 1.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:18715980"
FT CHAIN 24..90
FT /note="Serine protease inhibitor kazal-like protein, minor
FT form"
FT /evidence="ECO:0000269|PubMed:18715980"
FT /id="PRO_0000368186"
FT CHAIN 28..90
FT /note="Serine protease inhibitor kazal-like protein, major
FT form"
FT /evidence="ECO:0000269|PubMed:18715980"
FT /id="PRO_0000271263"
FT DOMAIN 33..89
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:18715980"
SQ SEQUENCE 90 AA; 10542 MW; 3A507D717D2C78D5 CRC64;
MSSTWIKFLF ILTLVLLPYS VFSVNIFAGP ENVIKEPNCT MYKSKSECSN IAENPVCADD
RNTYYNECYF CIEKVVEKLK YRYHGICIYK
