SPIN4_HUMAN
ID SPIN4_HUMAN Reviewed; 249 AA.
AC Q56A73; B3KX90; Q5JUL2;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Spindlin-4;
GN Name=SPIN4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND INTERACTION WITH C11ORF84/SPINDOC.
RX PubMed=29061846; DOI=10.1074/jbc.m117.814913;
RA Bae N., Gao M., Li X., Premkumar T., Sbardella G., Chen J., Bedford M.T.;
RT "A transcriptional coregulator, SPIN-DOC, attenuates the coactivator
RT activity of Spindlin1.";
RL J. Biol. Chem. 292:20808-20817(2017).
CC -!- FUNCTION: Exhibits H3K4me3-binding activity.
CC {ECO:0000269|PubMed:29061846}.
CC -!- SUBUNIT: Interacts with C11orf84/SPINDOC (PubMed:29061846).
CC {ECO:0000269|PubMed:29061846}.
CC -!- SIMILARITY: Belongs to the SPIN/STSY family. {ECO:0000305}.
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DR EMBL; AK126931; BAG54402.1; -; mRNA.
DR EMBL; AL158203; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC043412; AAH43412.1; -; mRNA.
DR EMBL; BC112360; AAI12361.1; -; mRNA.
DR CCDS; CCDS43964.1; -.
DR RefSeq; NP_001012986.2; NM_001012968.2.
DR PDB; 4UY4; X-ray; 1.86 A; A/B=36-249.
DR PDBsum; 4UY4; -.
DR AlphaFoldDB; Q56A73; -.
DR SMR; Q56A73; -.
DR BioGRID; 126590; 13.
DR IntAct; Q56A73; 1.
DR STRING; 9606.ENSP00000334163; -.
DR BindingDB; Q56A73; -.
DR ChEMBL; CHEMBL4523321; -.
DR GlyGen; Q56A73; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q56A73; -.
DR PhosphoSitePlus; Q56A73; -.
DR BioMuta; SPIN4; -.
DR DMDM; 74735974; -.
DR EPD; Q56A73; -.
DR MassIVE; Q56A73; -.
DR PaxDb; Q56A73; -.
DR PeptideAtlas; Q56A73; -.
DR PRIDE; Q56A73; -.
DR ProteomicsDB; 62580; -.
DR Antibodypedia; 52266; 79 antibodies from 13 providers.
DR DNASU; 139886; -.
DR Ensembl; ENST00000374884.3; ENSP00000364018.3; ENSG00000186767.7.
DR GeneID; 139886; -.
DR KEGG; hsa:139886; -.
DR MANE-Select; ENST00000374884.3; ENSP00000364018.3; NM_001012968.3; NP_001012986.2.
DR UCSC; uc004dvf.3; human.
DR CTD; 139886; -.
DR GeneCards; SPIN4; -.
DR HGNC; HGNC:27040; SPIN4.
DR HPA; ENSG00000186767; Low tissue specificity.
DR neXtProt; NX_Q56A73; -.
DR OpenTargets; ENSG00000186767; -.
DR PharmGKB; PA162404539; -.
DR VEuPathDB; HostDB:ENSG00000186767; -.
DR eggNOG; ENOG502QRYD; Eukaryota.
DR GeneTree; ENSGT00950000182925; -.
DR InParanoid; Q56A73; -.
DR OMA; HGRKDEW; -.
DR OrthoDB; 1027563at2759; -.
DR PhylomeDB; Q56A73; -.
DR TreeFam; TF332665; -.
DR PathwayCommons; Q56A73; -.
DR SignaLink; Q56A73; -.
DR BioGRID-ORCS; 139886; 13 hits in 675 CRISPR screens.
DR ChiTaRS; SPIN4; human.
DR GenomeRNAi; 139886; -.
DR Pharos; Q56A73; Tchem.
DR PRO; PR:Q56A73; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q56A73; protein.
DR Bgee; ENSG00000186767; Expressed in endothelial cell and 170 other tissues.
DR Genevisible; Q56A73; HS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0035064; F:methylated histone binding; IDA:UniProtKB.
DR GO; GO:0007276; P:gamete generation; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 2.80.10.70; -; 1.
DR InterPro; IPR003671; SPIN/Ssty.
DR InterPro; IPR042567; SPIN/Ssty_sf.
DR InterPro; IPR029578; Spindlin-4.
DR PANTHER; PTHR10405; PTHR10405; 1.
DR PANTHER; PTHR10405:SF9; PTHR10405:SF9; 1.
DR Pfam; PF02513; Spin-Ssty; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..249
FT /note="Spindlin-4"
FT /id="PRO_0000313794"
FT REGION 41..90
FT /note="Tudor-like domain 1"
FT /evidence="ECO:0000255"
FT REGION 119..168
FT /note="Tudor-like domain 2"
FT /evidence="ECO:0000255"
FT REGION 128
FT /note="Histone H3K4me3 and H3R8me2a binding"
FT /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT REGION 201..246
FT /note="Tudor-like domain 3"
FT /evidence="ECO:0000255"
FT REGION 237..239
FT /note="Histone H3K4me3 and H3R8me2a binding"
FT /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT SITE 159
FT /note="Histone H3K4me3 and H3R8me2a binding"
FT /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT SITE 170
FT /note="Histone H3K4me3 and H3R8me2a binding"
FT /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT CONFLICT 227
FT /note="P -> S (in Ref. 1; BAG54402)"
FT /evidence="ECO:0000305"
FT STRAND 44..49
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 57..66
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 74..78
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:4UY4"
FT TURN 89..91
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 95..101
FT /evidence="ECO:0007829|PDB:4UY4"
FT HELIX 112..118
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 122..127
FT /evidence="ECO:0007829|PDB:4UY4"
FT TURN 130..132
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 135..144
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 146..148
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 151..156
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 159..165
FT /evidence="ECO:0007829|PDB:4UY4"
FT HELIX 168..172
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 176..181
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 204..208
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 214..222
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 224..226
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 229..234
FT /evidence="ECO:0007829|PDB:4UY4"
FT STRAND 241..245
FT /evidence="ECO:0007829|PDB:4UY4"
SQ SEQUENCE 249 AA; 28660 MW; FF0CB0229107D8D4 CRC64;
MSPPTVPPMG VDGVSAYLMK KRHTHRKQRR KPTFLTRRNI VGCRIQHGWK EGNEPVEQWK
GTVLEQVSVK PTLYIIKYDG KDSVYGLELH RDKRVLALEI LPERVPTPRI DSRLADSLIG
KAVEHVFEGE HGTKDEWKGM VLARAPVMDT WFYITYEKDP VLYMYTLLDD YKDGDLRIIP
DSNYYFPTAE QEPGEVVDSL VGKQVEHAKD DGSKRTGIFI HQVVAKPSVY FIKFDDDIHI
YVYGLVKTP
