ID   SPING_PSEUS             Reviewed;          25 AA.
AC   P82357;
DT   04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT   04-MAY-2001, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Spinigerin;
DE   Contains:
DE     RecName: Full=Spinigerin N-3;
DE   Contains:
DE     RecName: Full=Spinigerin C-4;
OS   Pseudacanthotermes spiniger.
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Polyneoptera; Dictyoptera; Blattodea; Blattoidea; Termitoidae;
OC   Termitidae; Macrotermitinae; Pseudacanthotermes.
OX   NCBI_TaxID=115113;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND FUNCTION.
RC   TISSUE=Blood, and Salivary gland;
RX   PubMed=11053427; DOI=10.1074/jbc.m002998200;
RA   Lamberty M., Zachary D., Lanot R., Bordereau C., Robert A., Hoffmann J.A.,
RA   Bulet P.;
RT   "Insect immunity. Constitutive expression of a cysteine-rich antifungal and
RT   a linear antibacterial peptide in a termite insect.";
RL   J. Biol. Chem. 276:4085-4092(2001).
RN   [2]
RP   STRUCTURE BY NMR.
RX   PubMed=16170803; DOI=10.1002/bip.20370;
RA   Landon C., Meudal H., Boulanger N., Bulet P., Vovelle F.;
RT   "Solution structures of stomoxyn and spinigerin, two insect antimicrobial
RT   peptides with an alpha-helical conformation.";
RL   Biopolymers 81:92-103(2006).
CC   -!- FUNCTION: Active against Gram-positive bacteria B.megaterium and
CC       M.luteus, Gram-negative bacteria E.coli SBS363 and D22, K.pneumoniae,
CC       S.typhimurium and P.aeruginosa, yeast C.albicans and filamentous fungi
CC       F.culmorum, N.crassa, N.hematococca and T.viridae. Inactive against
CC       Gram-positive bacteria B.subtilis, S.pyogenes, B.thuringiensis and
CC       S.aureus, Gram-negative bacteria E.cloacae and E.carotovora and
CC       filamentous fungus B.bassiana. {ECO:0000269|PubMed:11053427}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- INDUCTION: By bacterial infection.
CC   -!- MASS SPECTROMETRY: [Spinigerin]: Mass=3001.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11053427};
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DR   PDB; 1ZRV; NMR; -; A=1-25.
DR   PDB; 1ZRW; NMR; -; A=1-25.
DR   PDBsum; 1ZRV; -.
DR   PDBsum; 1ZRW; -.
DR   AlphaFoldDB; P82357; -.
DR   SMR; P82357; -.
DR   EvolutionaryTrace; P82357; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Fungicide; Secreted.
FT   PEPTIDE         1..25
FT                   /note="Spinigerin"
FT                   /id="PRO_0000004947"
FT   PEPTIDE         1..21
FT                   /note="Spinigerin C-4"
FT                   /id="PRO_0000004948"
FT   PEPTIDE         4..25
FT                   /note="Spinigerin N-3"
FT                   /id="PRO_0000004949"
FT   HELIX           4..23
FT                   /evidence="ECO:0007829|PDB:1ZRV"
SQ   SEQUENCE   25 AA;  3001 MW;  AA79370264262F60 CRC64;
     HVDKKVADKV LLLKQLRIMR LLTRL