ID   SPIR_SPICI              Reviewed;         241 AA.
AC   P19215; Q53831;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   24-OCT-2003, sequence version 3.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Spiralin;
DE   Flags: Precursor;
GN   Name=spi;
OS   Spiroplasma citri.
OC   Bacteria; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC   Spiroplasma.
OX   NCBI_TaxID=2133;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27556 / NCPPB 2647 / R8A2;
RX   PubMed=2139649; DOI=10.1128/jb.172.5.2693-2703.1990;
RA   Chevalier C., Saillard C., Bove J.M.;
RT   "Organization and nucleotide sequences of the Spiroplasma citri genes for
RT   ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase,
RT   pyruvate kinase, and an unidentified protein.";
RL   J. Bacteriol. 172:2693-2703(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27556 / NCPPB 2647 / R8A2;
RX   PubMed=8631684; DOI=10.1128/jb.178.10.2934-2940.1996;
RA   Foissac X., Saillard C., Gandar J., Zreik L., Bove J.M.;
RT   "Spiralin polymorphism in strains of Spiroplasma citri is not due to
RT   differences in posttranslational palmitoylation.";
RL   J. Bacteriol. 178:2934-2940(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27556 / NCPPB 2647 / R8A2;
RX   PubMed=9732535; DOI=10.1007/s002849900377;
RA   Le Dantec L., Bove J.M., Saillard C.;
RT   "Gene organization and transcriptional analysis of the Spiroplasma citri
RT   rpsB/tsf/x operon.";
RL   Curr. Microbiol. 37:269-273(1998).
CC   -!- FUNCTION: Major membrane protein of spiroplasma.
CC   -!- SUBUNIT: Seems to occur as dimer, tetramers, and large oligomers of
CC       identical chains.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- PTM: Palmitate and stearate are the major lipid components.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the spiralin family. {ECO:0000305}.
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DR   EMBL; AF012877; AAB69997.1; -; Genomic_DNA.
DR   EMBL; U13998; AAB06630.1; -; Genomic_DNA.
DR   PIR; D35270; D35270.
DR   RefSeq; WP_071937535.1; NZ_CP047426.1.
DR   AlphaFoldDB; P19215; -.
DR   PRIDE; P19215; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007880; Spiralin.
DR   Pfam; PF05215; Spiralin; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           24..241
FT                   /note="Spiralin"
FT                   /id="PRO_0000022396"
FT   LIPID           24
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           24
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CONFLICT        200
FT                   /note="G -> E (in Ref. 2; AAB06630)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   241 AA;  25293 MW;  AF077C4064B798C6 CRC64;
     MKKLLSILAV FGVSAVGTTS VVACNKTESN NLSIVKTIAV PATVATANPK QVTNAEIKTA
     LEANVLKAVQ GVVKTATAAD FQFDVYQDNK GTSLTTINLE EGNVEVYVQI TPAKDKTVVI
     GETGYIKVTL PKIKVDISGV VIDQQIVEIK AADPKQVTKD ELNAVNTYAT LASAVLEAIK
     NKAPNAGASD FEITNNCDAG DYSAQKDVKV TVKAKDESPN ISGEFKVNAK VKATLAPPKA
     G