ID   SPIR_SPIME              Reviewed;         242 AA.
AC   P21625;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   24-OCT-2003, sequence version 2.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Spiralin;
DE   Flags: Precursor;
GN   Name=spi;
OS   Spiroplasma melliferum.
OC   Bacteria; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC   Spiroplasma.
OX   NCBI_TaxID=2134;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33219 / BC3;
RX   PubMed=2211527; DOI=10.1128/jb.172.10.6090-6097.1990;
RA   Chevalier C., Saillard C., Bove J.M.;
RT   "Spiralins of Spiroplasma citri and Spiroplasma melliferum: amino acid
RT   sequences and putative organization in the cell membrane.";
RL   J. Bacteriol. 172:6090-6097(1990).
RN   [2]
RP   PROTEOLYTIC PROCESSING OF N-TERMINUS, DIACYLGLYCEROL AT CYS-24, AND
RP   PALMITOYLATION AT CYS-24.
RX   PubMed=10896212; DOI=10.1007/s002030000145;
RA   Le Henaff M., Fontenelle C.;
RT   "Chemical analysis of processing of spiralin, the major lipoprotein of
RT   Spiroplasma melliferum.";
RL   Arch. Microbiol. 173:339-345(2000).
CC   -!- FUNCTION: Major membrane protein of spiroplasma.
CC   -!- SUBUNIT: Seems to occur as dimer, tetramers, and large oligomers of
CC       identical chains.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor.
CC   -!- PTM: Palmitate and stearate are the major lipid components.
CC       {ECO:0000269|PubMed:10896212}.
CC   -!- SIMILARITY: Belongs to the spiralin family. {ECO:0000305}.
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DR   EMBL; M59366; AAA26588.1; -; Genomic_DNA.
DR   PIR; A36149; A36149.
DR   AlphaFoldDB; P21625; -.
DR   STRING; 1129368.SMIPMB4A_v3c0060; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007880; Spiralin.
DR   Pfam; PF05215; Spiralin; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Signal.
FT   SIGNAL          1..23
FT   CHAIN           24..242
FT                   /note="Spiralin"
FT                   /id="PRO_0000022397"
FT   LIPID           24
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT                   ECO:0000269|PubMed:10896212"
FT   LIPID           24
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT                   ECO:0000269|PubMed:10896212"
SQ   SEQUENCE   242 AA;  25441 MW;  C10EC34ECCB5B93C CRC64;
     MKKLLSILAV FGVSAVGTTS VVACNKTESN NLSRVKTIAA PATVAASTPK AVTKPEIKTA
     LEANVLKAVQ GVVKTATAAD FQFEVYKNSK GTALETIDLE AGKVEVYLQI TPAKDKTVVI
     GETRYIKVTL PKHGEVTKVD IKDVTVTEQT VGIKASTPKA VKKDELNAVN TYATLAKAVL
     DAIQNIAPNA GASDFEITNN GAEGDYEAAK EVEVTVKAKN DSANISGQFK FKAKVTATAP
     TE