ID   SPISS_SANSP             Reviewed;         562 AA.
AC   E3W208;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=Sesquiterpene synthase;
DE            Short=SspiSesquiTPS;
DE            EC=4.2.3.-;
OS   Santalum spicatum (Australian sandalwood).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Santalales; Santalaceae; Santalum.
OX   NCBI_TaxID=453088;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   PubMed=21454632; DOI=10.1074/jbc.m111.231787;
RA   Jones C.G., Moniodis J., Zulak K.G., Scaffidi A., Plummer J.A.,
RA   Ghisalberti E.L., Barbour E.L., Bohlmann J.;
RT   "Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both
RT   the terpene synthase (TPS)-a and TPS-b Subfamilies, including santalene
RT   synthases.";
RL   J. Biol. Chem. 286:17445-17454(2011).
CC   -!- FUNCTION: Catalyzes the formation of beta-elemol, guaiol and bulnesol.
CC       {ECO:0000269|PubMed:21454632}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC       {ECO:0000305}.
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DR   EMBL; HQ343282; ADO87006.1; -; mRNA.
DR   AlphaFoldDB; E3W208; -.
DR   SMR; E3W208; -.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Magnesium; Manganese; Metal-binding.
FT   CHAIN           1..562
FT                   /note="Sesquiterpene synthase"
FT                   /id="PRO_0000418948"
FT   MOTIF           315..319
FT                   /note="DDXXD motif"
FT   BINDING         315
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         315
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         319
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         319
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         467
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   562 AA;  65873 MW;  8F7F7C4E0B4AFEBD CRC64;
     MENQKVPISS VPNLKDLDMI SRPIANFPPS VWGDRFINYA CEDENEQAQK ERQVEELKEQ
     VRRELAAAID KPLQQLNIID ATQRLGIAYH FENEIEESLK HIYLHTYVEN TCFEGSDDLC
     SVALWFRLLR QDGYRVSCDV FKKFRDSEGN FKNSLMEDAK GLLELYEATH LSVNGEEMLD
     DALEFTKTHL ELVVSHLNYP LAEQVRHALY QPQHKGLPRL EAVYFFRIYE AYDSHNEALL
     KLAKLDFNLL QSLHMKELSH MAKWWKSLDF ATKFPFARDR LVEGYFWILG VYFEPQYSLA
     RKIIIKVFTM ISTIDDIYDA YGTLDELKLF TKAIQRWDIG SLDQLPEYMK PCYKSVLDVY
     NEIEEEMDNQ GSLFRMHYAK EEMKKIVEGY MDEAKWCHEK YVPTFQEYMS VALVTAGYTF
     LTTISYLGMG EIASKEAFDW LFSHPPIIEA SESVGRLMDD MRSHEFEQER GHVASGIECY
     MKQYGVTEEE AHDKFHKRLV KAWKDINEGC LRPYPVPKPL LMRILSLTRV IDVIYKNEDW
     YTHVKKPMKD KIASLLIDPM IV