ID   SPIST_SANSP             Reviewed;         562 AA.
AC   F6M8H6;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Probable sesquiterpene synthase;
DE            Short=SspiSTPS;
DE            EC=4.2.3.-;
GN   Name=SesquiTPS;
OS   Santalum spicatum (Australian sandalwood).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Santalales; Santalaceae; Santalum.
OX   NCBI_TaxID=453088;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=21454632; DOI=10.1074/jbc.m111.231787;
RA   Jones C.G., Moniodis J., Zulak K.G., Scaffidi A., Plummer J.A.,
RA   Ghisalberti E.L., Barbour E.L., Bohlmann J.;
RT   "Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both
RT   the terpene synthase (TPS)-a and TPS-b Subfamilies, including santalene
RT   synthases.";
RL   J. Biol. Chem. 286:17445-17454(2011).
CC   -!- FUNCTION: Sesquiterpene synthase. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC       {ECO:0000305}.
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DR   EMBL; JF746809; AEF32536.1; -; Genomic_DNA.
DR   AlphaFoldDB; F6M8H6; -.
DR   SMR; F6M8H6; -.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase; Magnesium; Manganese; Metal-binding.
FT   CHAIN           1..562
FT                   /note="Probable sesquiterpene synthase"
FT                   /id="PRO_0000419327"
FT   MOTIF           315..319
FT                   /note="DDXXD motif"
FT   BINDING         315
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         315
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         319
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         319
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         467
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   562 AA;  66140 MW;  21F4CBD1994A5F8E CRC64;
     MENQKMPISS VSNLKDLNMI SRPVANFPPS IWGDRFINYA CEDENEQAQK ERQVEELKEQ
     VRRELATAID KPLQQLNIID ATQRLGIAYH FENEIEESLK HIYLHTYVEN TCFEGSDDLY
     SVALWFRLLR QDDYRVSCDV FKKFRDSEGN FKNSLMEDAK GLLELYEATH LSVNGEEMLD
     DALEFTKTHL ELVVSHLNYP LAEQVRHALY QPQHKGLPRL EAVYFFRIYE AYDSHNEALL
     KLAKLDFNLL QSLHMKELSH MAKWWKSLDF ATKFPFARDR LVEGYFWILG VYFEPQYSLA
     RKIIIKVFTM ISTIDDIYDA YGTLDELKLF TKAIQRWDIG SLDQLPEYMK PCYKSVLDVY
     NEIEEEMDNQ GSLFRMHYAK EEMKKIVEGY MDEAKWCHEK YVPTFQEYMS VALVTAGYTF
     LTTISYLGMG EIASKEAFDW LFSRPPIIEA SESVCRLMDD MRSHEFEQER GHVASGIECY
     MKQYGVTEEE AHDKFHKRLV KAWKDINEGC LRPYPMPKPL LMRILSLTRV IDVIYKNEDW
     YTHVKKPMKD KIASLLIDPM IV