SPIT3_HUMAN
ID SPIT3_HUMAN Reviewed; 89 AA.
AC P49223; A6NCQ6; Q6UDR8; Q96KK2;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 3.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Kunitz-type protease inhibitor 3;
DE AltName: Full=HKIB9;
DE Flags: Precursor;
GN Name=SPINT3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Epididymis;
RA Clauss A., Lundwall A.;
RT "Several Kunitz-type serine proteinase inhibitors are expressed in the
RT epididymis.";
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-89.
RC TISSUE=Blood, and Placenta;
RA Norris F., Norris K., Bjoern S.E., Petersen L.C., Foster D.C.,
RA Sprecher C.A.;
RT "A novel human Kunitz-type protease inhibitor and variants thereof.";
RL Patent number WO9314123, 22-JUL-1993.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
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DR EMBL; AY372172; AAR17081.2; -; mRNA.
DR EMBL; AL031663; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; X77166; CAA54410.1; -; Genomic_DNA.
DR CCDS; CCDS46608.1; -.
DR PIR; S41399; S41399.
DR RefSeq; NP_006643.1; NM_006652.1.
DR AlphaFoldDB; P49223; -.
DR SMR; P49223; -.
DR BioGRID; 116029; 1.
DR STRING; 9606.ENSP00000217428; -.
DR MEROPS; I02.969; -.
DR BioMuta; SPINT3; -.
DR DMDM; 152031689; -.
DR MassIVE; P49223; -.
DR PaxDb; P49223; -.
DR PeptideAtlas; P49223; -.
DR PRIDE; P49223; -.
DR ProteomicsDB; 55972; -.
DR Antibodypedia; 72138; 7 antibodies from 4 providers.
DR DNASU; 10816; -.
DR Ensembl; ENST00000217428.7; ENSP00000217428.6; ENSG00000101446.8.
DR GeneID; 10816; -.
DR KEGG; hsa:10816; -.
DR MANE-Select; ENST00000217428.7; ENSP00000217428.6; NM_006652.2; NP_006643.1.
DR UCSC; uc010ghg.1; human.
DR CTD; 10816; -.
DR DisGeNET; 10816; -.
DR GeneCards; SPINT3; -.
DR HGNC; HGNC:11248; SPINT3.
DR HPA; ENSG00000101446; Group enriched (epididymis, seminal vesicle).
DR MIM; 613941; gene.
DR neXtProt; NX_P49223; -.
DR OpenTargets; ENSG00000101446; -.
DR PharmGKB; PA36078; -.
DR VEuPathDB; HostDB:ENSG00000101446; -.
DR eggNOG; KOG4295; Eukaryota.
DR GeneTree; ENSGT00940000163688; -.
DR HOGENOM; CLU_164133_0_3_1; -.
DR InParanoid; P49223; -.
DR OMA; VCAFPME; -.
DR OrthoDB; 1564520at2759; -.
DR PhylomeDB; P49223; -.
DR TreeFam; TF327278; -.
DR PathwayCommons; P49223; -.
DR BioGRID-ORCS; 10816; 10 hits in 1055 CRISPR screens.
DR ChiTaRS; SPINT3; human.
DR GenomeRNAi; 10816; -.
DR Pharos; P49223; Tdark.
DR PRO; PR:P49223; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; P49223; protein.
DR Bgee; ENSG00000101446; Expressed in cauda epididymis and 26 other tissues.
DR Genevisible; P49223; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0048019; F:receptor antagonist activity; IBA:GO_Central.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0050431; F:transforming growth factor beta binding; IBA:GO_Central.
DR GO; GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IBA:GO_Central.
DR CDD; cd00109; KU; 1.
DR Gene3D; 4.10.410.10; -; 1.
DR InterPro; IPR002223; Kunitz_BPTI.
DR InterPro; IPR036880; Kunitz_BPTI_sf.
DR InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR Pfam; PF00014; Kunitz_BPTI; 1.
DR PRINTS; PR00759; BASICPTASE.
DR SMART; SM00131; KU; 1.
DR SUPFAM; SSF57362; SSF57362; 1.
DR PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..89
FT /note="Kunitz-type protease inhibitor 3"
FT /id="PRO_0000016887"
FT DOMAIN 36..86
FT /note="BPTI/Kunitz inhibitor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DISULFID 36..86
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DISULFID 45..69
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DISULFID 61..82
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT VARIANT 77
FT /note="L -> S (in dbSNP:rs6032259)"
FT /id="VAR_059443"
SQ SEQUENCE 89 AA; 10252 MW; C9C471371CD77A9F CRC64;
MQLQASLSFL LILTLCLELR SELARDTIKD LLPNVCAFPM EKGPCQTYMT RWFFNFETGE
CELFAYGGCG GNSNNFLRKE KCEKFCKFT
