ID   SPIT4_HUMAN             Reviewed;          99 AA.
AC   Q6UDR6; Q9BQN3;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Kunitz-type protease inhibitor 4;
DE   Flags: Precursor;
GN   Name=SPINT4; Synonyms=C20orf137;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Epididymis;
RA   Clauss A., Lundwall A.;
RT   "Several Kunitz-type serine proteinase inhibitors are expressed in the
RT   epididymis.";
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11780052; DOI=10.1038/414865a;
RA   Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA   Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA   Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA   Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA   Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA   Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA   Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA   Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA   Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA   Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA   Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA   Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA   Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA   Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA   Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA   Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA   Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA   Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA   Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA   Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA   Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA   Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA   Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT   "The DNA sequence and comparative analysis of human chromosome 20.";
RL   Nature 414:865-871(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
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DR   EMBL; AY372173; AAR17082.1; -; mRNA.
DR   EMBL; AY372174; AAR17083.1; -; mRNA.
DR   EMBL; AL109656; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471077; EAW75818.1; -; Genomic_DNA.
DR   CCDS; CCDS33477.1; -.
DR   RefSeq; NP_848550.1; NM_178455.2.
DR   AlphaFoldDB; Q6UDR6; -.
DR   SMR; Q6UDR6; -.
DR   STRING; 9606.ENSP00000279058; -.
DR   MEROPS; I02.975; -.
DR   GlyGen; Q6UDR6; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q6UDR6; -.
DR   PhosphoSitePlus; Q6UDR6; -.
DR   BioMuta; SPINT4; -.
DR   DMDM; 74749362; -.
DR   MassIVE; Q6UDR6; -.
DR   PaxDb; Q6UDR6; -.
DR   PeptideAtlas; Q6UDR6; -.
DR   PRIDE; Q6UDR6; -.
DR   Antibodypedia; 78024; 4 antibodies from 4 providers.
DR   DNASU; 391253; -.
DR   Ensembl; ENST00000279058.4; ENSP00000279058.3; ENSG00000149651.4.
DR   GeneID; 391253; -.
DR   KEGG; hsa:391253; -.
DR   MANE-Select; ENST00000279058.4; ENSP00000279058.3; NM_178455.3; NP_848550.1.
DR   UCSC; uc002xpe.2; human.
DR   CTD; 391253; -.
DR   DisGeNET; 391253; -.
DR   GeneCards; SPINT4; -.
DR   HGNC; HGNC:16130; SPINT4.
DR   HPA; ENSG00000149651; Tissue enriched (epididymis).
DR   MIM; 619430; gene.
DR   neXtProt; NX_Q6UDR6; -.
DR   PharmGKB; PA25679; -.
DR   VEuPathDB; HostDB:ENSG00000149651; -.
DR   eggNOG; KOG4295; Eukaryota.
DR   GeneTree; ENSGT00390000004362; -.
DR   HOGENOM; CLU_164133_0_3_1; -.
DR   InParanoid; Q6UDR6; -.
DR   OMA; FKLKIEC; -.
DR   OrthoDB; 1552121at2759; -.
DR   PhylomeDB; Q6UDR6; -.
DR   TreeFam; TF338169; -.
DR   PathwayCommons; Q6UDR6; -.
DR   BioGRID-ORCS; 391253; 13 hits in 1047 CRISPR screens.
DR   GenomeRNAi; 391253; -.
DR   Pharos; Q6UDR6; Tdark.
DR   PRO; PR:Q6UDR6; -.
DR   Proteomes; UP000005640; Chromosome 20.
DR   RNAct; Q6UDR6; protein.
DR   Bgee; ENSG00000149651; Expressed in urinary bladder and 18 other tissues.
DR   ExpressionAtlas; Q6UDR6; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR042943; SPINT4.
DR   PANTHER; PTHR47898; PTHR47898; 1.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..99
FT                   /note="Kunitz-type protease inhibitor 4"
FT                   /id="PRO_0000308327"
FT   DOMAIN          41..91
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        41..91
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        50..74
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   VARIANT         30
FT                   /note="A -> E (in dbSNP:rs16990631)"
FT                   /id="VAR_050068"
FT   VARIANT         73
FT                   /note="G -> S (in dbSNP:rs6017667)"
FT                   /id="VAR_036795"
SQ   SEQUENCE   99 AA;  11421 MW;  9963933C1C82737D CRC64;
     MKSAKLGFLL RFFIFCSLNT LLLGGVNKIA EKICGDLKDP CKLDMNFGSC YEVHFRYFYN
     RTSKRCETFV FSGCNGNLNN FKLKIEREVA CVAKYKPPR