SPIT4_MOUSE
ID SPIT4_MOUSE Reviewed; 159 AA.
AC Q9D263;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Kunitz-type protease inhibitor 4;
DE AltName: Full=Epididymal trypsin inhibitor Spint4;
DE Flags: Precursor;
GN Name=Spint4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=12920233; DOI=10.1210/me.2003-0008;
RA Penttinen J., Pujianto D.A., Sipilae P., Huhtaniemi I., Poutanen M.;
RT "Discovery in silico and characterization in vitro of novel genes
RT exclusively expressed in the mouse epididymis.";
RL Mol. Endocrinol. 17:2138-2151(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Epididymis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the epididymis, in the
CC epithelial cells of the distal caput and early corpus.
CC {ECO:0000269|PubMed:12920233}.
CC -!- DEVELOPMENTAL STAGE: Expression starts at the time of epididymal
CC maturation. {ECO:0000269|PubMed:12920233}.
CC -!- INDUCTION: By androgens. Expression diminishes after 1-3 days and
CC disappears 7 days postgonadectomy. {ECO:0000269|PubMed:12920233}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY226988; AAP43945.1; -; mRNA.
DR EMBL; AK020315; BAB32069.1; -; mRNA.
DR EMBL; AL591127; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC119299; AAI19300.1; -; mRNA.
DR EMBL; BC119301; AAI19302.1; -; mRNA.
DR CCDS; CCDS17051.1; -.
DR RefSeq; NP_084334.1; NM_030058.2.
DR AlphaFoldDB; Q9D263; -.
DR SMR; Q9D263; -.
DR STRING; 10090.ENSMUSP00000017454; -.
DR MEROPS; I02.975; -.
DR PhosphoSitePlus; Q9D263; -.
DR PaxDb; Q9D263; -.
DR PRIDE; Q9D263; -.
DR ProteomicsDB; 261623; -.
DR Antibodypedia; 78024; 4 antibodies from 4 providers.
DR DNASU; 78239; -.
DR Ensembl; ENSMUST00000017454; ENSMUSP00000017454; ENSMUSG00000017310.
DR GeneID; 78239; -.
DR KEGG; mmu:78239; -.
DR UCSC; uc008nvr.1; mouse.
DR CTD; 391253; -.
DR MGI; MGI:1925489; Spint4.
DR VEuPathDB; HostDB:ENSMUSG00000017310; -.
DR eggNOG; KOG4295; Eukaryota.
DR GeneTree; ENSGT00390000004362; -.
DR HOGENOM; CLU_1660141_0_0_1; -.
DR InParanoid; Q9D263; -.
DR OMA; FKLKIEC; -.
DR OrthoDB; 1445655at2759; -.
DR PhylomeDB; Q9D263; -.
DR TreeFam; TF338169; -.
DR BioGRID-ORCS; 78239; 2 hits in 70 CRISPR screens.
DR PRO; PR:Q9D263; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9D263; protein.
DR Bgee; ENSMUSG00000017310; Expressed in morula and 7 other tissues.
DR Genevisible; Q9D263; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00109; KU; 1.
DR Gene3D; 4.10.410.10; -; 1.
DR InterPro; IPR002223; Kunitz_BPTI.
DR InterPro; IPR036880; Kunitz_BPTI_sf.
DR InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR InterPro; IPR042943; SPINT4.
DR PANTHER; PTHR47898; PTHR47898; 1.
DR Pfam; PF00014; Kunitz_BPTI; 1.
DR PRINTS; PR00759; BASICPTASE.
DR SMART; SM00131; KU; 1.
DR SUPFAM; SSF57362; SSF57362; 1.
DR PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..159
FT /note="Kunitz-type protease inhibitor 4"
FT /id="PRO_0000308328"
FT DOMAIN 41..91
FT /note="BPTI/Kunitz inhibitor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DISULFID 41..91
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DISULFID 50..74
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DISULFID 66..87
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ SEQUENCE 159 AA; 18327 MW; 231D1326B666B1BE CRC64;
MKPTKLGFLL GLSLLCSLSP PVLSGVERLA NYLCKDYNDP CLLDVEPGSC YEVHFRFFYN
QTAKQCQIFL FTGCNGNLNN FKLKIDCDVT CHETYKNPPL PSGDKRKRSL RALATKNLAT
DWRLTTLQPG RLRQTERKET LLQRARRESR RQPLARHHT
