SPL12_ARATH
ID SPL12_ARATH Reviewed; 927 AA.
AC Q9S7P5;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Squamosa promoter-binding-like protein 12;
GN Name=SPL12; OrderedLocusNames=At3g60030; ORFNames=T2O9.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=10524240; DOI=10.1016/s0378-1119(99)00308-x;
RA Cardon G.H., Hoehmann S., Klein J., Nettesheim K., Saedler H., Huijser P.;
RT "Molecular characterization of the Arabidopsis SBP-box genes.";
RL Gene 237:91-104(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, REGION, STRUCTURE BY NMR OF 124-181, AND ZINC-BINDING SITES
RP CYS-127; CYS-132; CYS-149 AND HIS-152.
RX PubMed=16554053; DOI=10.1016/j.febslet.2006.03.014;
RA Yamasaki K., Kigawa T., Inoue M., Yamasaki T., Yabuki T., Aoki M., Seki E.,
RA Matsuda T., Tomo Y., Terada T., Shirouzu M., Tanaka A., Seki M.,
RA Shinozaki K., Yokoyama S.;
RT "An Arabidopsis SBP-domain fragment with a disrupted C-terminal zinc-
RT binding site retains its tertiary structure.";
RL FEBS Lett. 580:2109-2116(2006).
CC -!- FUNCTION: Trans-acting factor that binds specifically to the consensus
CC nucleotide sequence 5'-TNCGTACAA-3'. {ECO:0000269|PubMed:16554053}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed during plant development.
CC {ECO:0000269|PubMed:10524240}.
CC -!- DOMAIN: The SBP-type zinc finger is required for the binding to DNA.
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DR EMBL; AJ132096; CAB56768.1; -; mRNA.
DR EMBL; AJ132097; CAB56769.1; -; Genomic_DNA.
DR EMBL; AL138658; CAB75918.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE80004.1; -; Genomic_DNA.
DR EMBL; BT003817; AAO41870.1; -; mRNA.
DR PIR; T47827; T47827.
DR RefSeq; NP_191562.1; NM_115866.4.
DR PDB; 1WJ0; NMR; -; A=124-181.
DR PDBsum; 1WJ0; -.
DR AlphaFoldDB; Q9S7P5; -.
DR SMR; Q9S7P5; -.
DR STRING; 3702.AT3G60030.1; -.
DR PaxDb; Q9S7P5; -.
DR PRIDE; Q9S7P5; -.
DR ProteomicsDB; 228458; -.
DR EnsemblPlants; AT3G60030.1; AT3G60030.1; AT3G60030.
DR GeneID; 825173; -.
DR Gramene; AT3G60030.1; AT3G60030.1; AT3G60030.
DR KEGG; ath:AT3G60030; -.
DR Araport; AT3G60030; -.
DR TAIR; locus:2101402; AT3G60030.
DR eggNOG; ENOG502QS71; Eukaryota.
DR HOGENOM; CLU_006255_3_0_1; -.
DR InParanoid; Q9S7P5; -.
DR OMA; RQDEASW; -.
DR OrthoDB; 216262at2759; -.
DR PhylomeDB; Q9S7P5; -.
DR EvolutionaryTrace; Q9S7P5; -.
DR PRO; PR:Q9S7P5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9S7P5; baseline and differential.
DR Genevisible; Q9S7P5; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..927
FT /note="Squamosa promoter-binding-like protein 12"
FT /id="PRO_0000132733"
FT ZN_FING 124..201
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT REGION 124..200
FT /note="Sufficient and necessary for DNA binding"
FT REGION 191..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 343..400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 184..200
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 350..400
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 127
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 132
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 149
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 152
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 168
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 171
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 175
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 187
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT TURN 143..147
FT /evidence="ECO:0007829|PDB:1WJ0"
FT HELIX 150..153
FT /evidence="ECO:0007829|PDB:1WJ0"
FT STRAND 158..160
FT /evidence="ECO:0007829|PDB:1WJ0"
FT STRAND 163..165
FT /evidence="ECO:0007829|PDB:1WJ0"
FT STRAND 169..171
FT /evidence="ECO:0007829|PDB:1WJ0"
SQ SEQUENCE 927 AA; 104142 MW; 69354E78FA56B940 CRC64;
MEARIEGEVE GHSLEYGFSG KRSVEWDLND WKWNGDLFVA TQLNHGSSNS SSTCSDEGNV
EIMERRRIEM EKKKKRRAVT VVAMEEDNLK DDDAHRLTLN LGGNNIEGNG VKKTKLGGGI
PSRAICCQVD NCGADLSKVK DYHRRHKVCE IHSKATTALV GGIMQRFCQQ CSRFHVLEEF
DEGKRSCRRR LAGHNKRRRK ANPDTIGNGT SMSDDQTSNY MLITLLKILS NIHSNQSDQT
GDQDLLSHLL KSLVSQAGEH IGRNLVGLLQ GGGGLQASQN IGNLSALLSL EQAPREDIKH
HSVSETPWQE VYANSAQERV APDRSEKQVK VNDFDLNDIY IDSDDTTDIE RSSPPPTNPA
TSSLDYHQDS RQSSPPQTSR RNSDSASDQS PSSSSGDAQS RTDRIVFKLF GKEPNDFPVA
LRGQILNWLA HTPTDMESYI RPGCIVLTIY LRQDEASWEE LCCDLSFSLR RLLDLSDDPL
WTDGWLYLRV QNQLAFAFNG QVVLDTSLPL RSHDYSQIIT VRPLAVTKKA QFTVKGINLR
RPGTRLLCTV EGTHLVQEAT QGGMEERDDL KENNEIDFVN FSCEMPIASG RGFMEIEDQG
GLSSSFFPFI VSEDEDICSE IRRLESTLEF TGTDSAMQAM DFIHEIGWLL HRSELKSRLA
ASDHNPEDLF SLIRFKFLIE FSMDREWCCV MKKLLNILFE EGTVDPSPDA ALSELCLLHR
AVRKNSKPMV EMLLRFSPKK KNQTLAGLFR PDAAGPGGLT PLHIAAGKDG SEDVLDALTE
DPGMTGIQAW KNSRDNTGFT PEDYARLRGH FSYIHLVQRK LSRKPIAKEH VVVNIPESFN
IEHKQEKRSP MDSSSLEITQ INQCKLCDHK RVFVTTHHKS VAYRPAMLSM VAIAAVCVCV
ALLFKSCPEV LYVFQPFRWE LLEYGTS
