SPL15_ARAAL
ID SPL15_ARAAL Reviewed; 355 AA.
AC S4WUL1;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 1.
DT 03-AUG-2022, entry version 31.
DE RecName: Full=Squamosa promoter-binding protein-like 15 {ECO:0000303|PubMed:30679374};
DE Short=AaSPL15 {ECO:0000303|PubMed:30679374};
GN Name=SPL15 {ECO:0000303|PubMed:23723236};
GN OrderedLocusNames=AALP_Aa5g233200 {ECO:0000312|EMBL:KFK35084.1};
OS Arabis alpina (Alpine rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis.
OX NCBI_TaxID=50452;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=23723236; DOI=10.1126/science.1234116;
RA Bergonzi S., Albani M.C., Ver Loren van Themaat E., Nordstrom K.J.,
RA Wang R., Schneeberger K., Moerland P.D., Coupland G.;
RT "Mechanisms of age-dependent response to winter temperature in perennial
RT flowering of Arabis alpina.";
RL Science 340:1094-1097(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pajares;
RX PubMed=27246759; DOI=10.1038/nplants.2014.23;
RA Willing E.M., Rawat V., Mandakova T., Maumus F., James G.V.,
RA Nordstroem K.J., Becker C., Warthmann N., Chica C., Szarzynska B.,
RA Zytnicki M., Albani M.C., Kiefer C., Bergonzi S., Castaings L.,
RA Mateos J.L., Berns M.C., Bujdoso N., Piofczyk T., de Lorenzo L.,
RA Barrero-Sicilia C., Mateos I., Piednoel M., Hagmann J., Chen-Min-Tao R.,
RA Iglesias-Fernandez R., Schuster S.C., Alonso-Blanco C., Roudier F.,
RA Carbonero P., Paz-Ares J., Davis S.J., Pecinka A., Quesneville H.,
RA Colot V., Lysak M.A., Weigel D., Coupland G., Schneeberger K.;
RT "Genome expansion of Arabis alpina linked with retrotransposition and
RT reduced symmetric DNA methylation.";
RL Nat. Plants 1:14023-14023(2015).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=30679374; DOI=10.1126/science.aau8197;
RA Hyun Y., Vincent C., Tilmes V., Bergonzi S., Kiefer C., Richter R.,
RA Martinez-Gallegos R., Severing E., Coupland G.;
RT "A regulatory circuit conferring varied flowering response to cold in
RT annual and perennial plants.";
RL Science 363:409-412(2019).
CC -!- FUNCTION: Probable transcription factor required for the flowering
CC response to vernalization in the shoot apical meristem (SAM)
CC (PubMed:30679374). Defines the competence of shoot meristems to flower
CC in response to vernalization in perennials (PubMed:30679374).
CC {ECO:0000269|PubMed:30679374}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Induced in the shoot apical meristem (SAM) during
CC vernalization. {ECO:0000269|PubMed:30679374}.
CC -!- DOMAIN: The SBP-type zinc finger is required for the binding to DNA.
CC {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Spl15 mutant plants fail to flower after
CC vernalization. {ECO:0000269|PubMed:30679374}.
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DR EMBL; KC815962; AGP03038.1; -; Genomic_DNA.
DR EMBL; CM002873; KFK35084.1; -; Genomic_DNA.
DR AlphaFoldDB; S4WUL1; -.
DR SMR; S4WUL1; -.
DR STRING; 50452.S4WUL1; -.
DR EnsemblPlants; KFK35084; KFK35084; AALP_AA5G233200.
DR Gramene; KFK35084; KFK35084; AALP_AA5G233200.
DR eggNOG; ENOG502QPVZ; Eukaryota.
DR OMA; MEPENTR; -.
DR OrthoDB; 910635at2759; -.
DR Proteomes; UP000029120; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0048653; P:anther development; IEA:EnsemblPlants.
DR GO; GO:0010220; P:positive regulation of vernalization response; IMP:UniProtKB.
DR GO; GO:0042127; P:regulation of cell population proliferation; IEA:EnsemblPlants.
DR GO; GO:0008361; P:regulation of cell size; IEA:EnsemblPlants.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Flowering; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..355
FT /note="Squamosa promoter-binding protein-like 15"
FT /id="PRO_0000447442"
FT ZN_FING 61..138
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 121..137
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 7..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 64
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 69
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 86
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 89
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 105
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 108
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 112
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 124
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
SQ SEQUENCE 355 AA; 39410 MW; A0AC6E7CE25E9771 CRC64;
MELLKGSGLN QTESGGSSST ESSSLSGGLR FGQKIYFEDG SGSGSGSSKN RVHNTGRKSL
TARCQVEGCR MDLTNAKSYY SRHKVCCIHS KSSKVIVSGL PQRFCQQCSR FHLLSEFDLE
KRSCRRRLAC HNERRRKPQA TTSLLSSRYA RTAPSLYGNA NSAMIRSVLG DPTAWATARS
AMRWSGPERE SHQVMNVFSS HGSSSFTTTC PEMMMNNNGT DSSCALSLLS NTNPNQQLQQ
HQLQTPTNVW RPSSGFNPVI ADRVTMAQPP PVSIHNQYLN NQTWEFTTGE KSNLQYMSPV
LGPSQISQPA DFQISNGSTM GGFELSHHQQ VLRQYMEPEN TRAYDSSPQH FNWSL
