SPL16_ARATH
ID SPL16_ARATH Reviewed; 988 AA.
AC Q700C2; Q8W4C0; Q9C9K0; Q9SMW9;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Squamosa promoter-binding-like protein 16;
DE AltName: Full=SPL1-related protein 3;
GN Name=SPL16; Synonyms=SPL1R3; OrderedLocusNames=At1g76580;
GN ORFNames=F14G6.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 180-682 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=15208423; DOI=10.1104/pp.104.042176;
RA Gong W., Shen Y.-P., Ma L.-G., Pan Y., Du Y.-L., Wang D.-H., Yang J.-Y.,
RA Hu L.-D., Liu X.-F., Dong C.-X., Ma L., Chen Y.-H., Yang X.-Y., Gao Y.,
RA Zhu D., Tan X., Mu J.-Y., Zhang D.-B., Liu Y.-L., Dinesh-Kumar S.P., Li Y.,
RA Wang X.-P., Gu H.-Y., Qu L.-J., Bai S.-N., Lu Y.-T., Li J.-Y., Zhao J.-D.,
RA Zuo J., Huang H., Deng X.-W., Zhu Y.-X.;
RT "Genome-wide ORFeome cloning and analysis of Arabidopsis transcription
RT factor genes.";
RL Plant Physiol. 135:773-782(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 342-988 (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=10524240; DOI=10.1016/s0378-1119(99)00308-x;
RA Cardon G.H., Hoehmann S., Klein J., Nettesheim K., Saedler H., Huijser P.;
RT "Molecular characterization of the Arabidopsis SBP-box genes.";
RL Gene 237:91-104(1999).
CC -!- FUNCTION: Trans-acting factor that binds specifically to the consensus
CC nucleotide sequence 5'-TNCGTACAA-3'. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q700C2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q700C2-2; Sequence=VSP_013986, VSP_013987, VSP_013988;
CC -!- MISCELLANEOUS: [Isoform 1]: May be due to a competing acceptor splice
CC site.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51947.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC015450; AAG51947.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AY062670; AAL32748.1; -; mRNA.
DR EMBL; BT006626; AAP31970.1; -; mRNA.
DR EMBL; AJ630503; CAG25876.1; -; mRNA.
DR EMBL; AY568676; AAS79566.1; -; mRNA.
DR EMBL; AJ242958; CAB56771.1; -; mRNA.
DR PIR; H96793; H96793.
DR PIR; T52568; T52568.
DR AlphaFoldDB; Q700C2; -.
DR STRING; 3702.AT1G76580.1; -.
DR iPTMnet; Q700C2; -.
DR PaxDb; Q700C2; -.
DR PRIDE; Q700C2; -.
DR Araport; AT1G76580; -.
DR TAIR; locus:2011706; AT1G76580.
DR eggNOG; ENOG502QS71; Eukaryota.
DR HOGENOM; CLU_006255_3_0_1; -.
DR InParanoid; Q700C2; -.
DR PhylomeDB; Q700C2; -.
DR PRO; PR:Q700C2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q700C2; baseline and differential.
DR Genevisible; Q700C2; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..988
FT /note="Squamosa promoter-binding-like protein 16"
FT /id="PRO_0000132737"
FT ZN_FING 79..156
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT REGION 52..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 240..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 289..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 139..155
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 245..262
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..316
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..396
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 82
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 87
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 104
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 107
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 123
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 126
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 130
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 142
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT VAR_SEQ 1..179
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_013986"
FT VAR_SEQ 660..667
FT /note="LENGLRGD -> PMPRFAKS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_013987"
FT VAR_SEQ 668..988
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_013988"
SQ SEQUENCE 988 AA; 109849 MW; 5126C13009213A78 CRC64;
MGELPKDDWQ MNRWKWDGQR FEAIELQGES LQLSNKKGLD LNLPCGFNDV EGTPVDLTRP
SKKVRSGSPG SGGGGGGNYP KCQVDNCKED LSIAKDYHRR HKVCEVHSKA TKALVGKQMQ
RFCQQCSRFH LLSEFDEGKR SCRRRLDGHN RRRRKTQPDA ITSQVVALEN RDNTSNNTNM
DVMALLTALV CAQGRNEATT NGSPGVPQRE QLLQILNKIK ALPLPMNLTS KLNNIGILAR
KNPEQPSPMN PQNSMNGASS PSTMDLLAAL SASLGSSAPE AIAFLSQGGF GNKESNDRTK
LTSSDHSATT SLEKKTLEFP SFGGGERTSS TNHSPSQYSD SRGQDTRSSL SLQLFTSSPE
EESRPKVASS TKYYSSASSN PVEDRSPSSS PVMQELFPLH TSPETRRYNN YKDTSTSPRT
SCLPLELFGA SNRGATANPN YNVLRHQSGY ASSGSDYSPP SLNSNAQERT GKISFKLFEK
DPSQLPNTLR TEIFRWLSSF PSDMESFIRP GCVILSVYVA MSASAWEQLE ENLLQRVRSL
VQDSEFWSNS RFLVNAGRQL ASHKHGRIRL SKSWRTLNLP ELITVSPLAV VAGEETALIV
RGRNLTNDGM RLRCAHMGNY ASMEVTGREH RLTKVDELNV SSFQVQSASS VSLGRCFIEL
ENGLRGDNFP LIIANATICK ELNRLEEEFH PKDVIEEQIQ NLDRPRSREE VLCFLNELGW
LFQRKWTSDI HGEPDFSLPR FKFLLVCSVE RDYCSLIRTV LDMMVERNLG KDGLLNKESL
DMLADIQLLN RAIKRRNTKM AETLIHYSVN PSTRNFIFLP SIAGPGDITP LHLAASTSSS
DDMIDALTND PQEIGLSCWN TLVDATGQTP FSYAAMRDNH SYNTLVARKL ADKRNGQISL
NIENGIDQIG LSKRLSSELK RSCNTCASVA LKYQRKVSGS RRLFPTPIIH SMLAVATVCV
CVCVFMHAFP MVRQGSHFSW GGLDYGSI
