SPL2_YEAST
ID SPL2_YEAST Reviewed; 148 AA.
AC P38839; D3DL85; P87290;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Putative cyclin-dependent kinase inhibitor SPL2;
DE AltName: Full=Suppressor of PLC1 deletion protein 2;
GN Name=SPL2; OrderedLocusNames=YHR136C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sigma 1278B;
RX PubMed=9491083; DOI=10.1007/s004380050644;
RA Iraqui I., Vissers S., Cartiaux M., Urrestarazu A.;
RT "Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding
RT aromatic aminotransferases I and II reveals a new aminotransferase
RT subfamily.";
RL Mol. Gen. Genet. 257:238-248(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION, AND INDUCTION.
RX PubMed=9475719; DOI=10.1093/genetics/148.1.33;
RA Flick J.S., Thorner J.;
RT "An essential function of a phosphoinositide-specific phospholipase C is
RT relieved by inhibition of a cyclin-dependent protein kinase in the yeast
RT Saccharomyces cerevisiae.";
RL Genetics 148:33-47(1998).
RN [6]
RP INDUCTION.
RX PubMed=11102525; DOI=10.1091/mbc.11.12.4309;
RA Ogawa N., DeRisi J.L., Brown P.O.;
RT "New components of a system for phosphate accumulation and polyphosphate
RT metabolism in Saccharomyces cerevisiae revealed by genomic expression
RT analysis.";
RL Mol. Biol. Cell 11:4309-4321(2000).
RN [7]
RP INDUCTION.
RX PubMed=12414795; DOI=10.1074/jbc.m209759200;
RA Boer V.M., de Winde J.H., Pronk J.T., Piper M.D.W.;
RT "The genome-wide transcriptional responses of Saccharomyces cerevisiae
RT grown on glucose in aerobic chemostat cultures limited for carbon,
RT nitrogen, phosphorus, or sulfur.";
RL J. Biol. Chem. 278:3265-3274(2003).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59 AND SER-86, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [11]
RP FUNCTION.
RX PubMed=17889672; DOI=10.1016/j.molcel.2007.07.022;
RA Wykoff D.D., Rizvi A.H., Raser J.M., Margolin B., O'Shea E.K.;
RT "Positive feedback regulates switching of phosphate transporters in S.
RT cerevisiae.";
RL Mol. Cell 27:1005-1013(2007).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Putative cyclin-dependent kinase (CDK) inhibitor necessary
CC and sufficient for PHO pathway-dependent down-regulation of low-
CC affinity phosphate transport. {ECO:0000269|PubMed:17889672,
CC ECO:0000269|PubMed:9475719}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic granule
CC {ECO:0000269|PubMed:14562095}. Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- INDUCTION: Regulated by phosphate, probably through its PHO4-binding
CC elements in the promoter. {ECO:0000269|PubMed:11102525,
CC ECO:0000269|PubMed:12414795, ECO:0000269|PubMed:9475719}.
CC -!- MISCELLANEOUS: Present with 2180 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Y13625; CAA73949.1; -; Genomic_DNA.
DR EMBL; U10398; AAB68421.1; -; Genomic_DNA.
DR EMBL; AY693121; AAT93140.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06829.1; -; Genomic_DNA.
DR PIR; S48980; S48980.
DR RefSeq; NP_012004.1; NM_001179266.1.
DR AlphaFoldDB; P38839; -.
DR BioGRID; 36569; 53.
DR DIP; DIP-4089N; -.
DR IntAct; P38839; 2.
DR MINT; P38839; -.
DR STRING; 4932.YHR136C; -.
DR iPTMnet; P38839; -.
DR MaxQB; P38839; -.
DR PaxDb; P38839; -.
DR PRIDE; P38839; -.
DR EnsemblFungi; YHR136C_mRNA; YHR136C; YHR136C.
DR GeneID; 856538; -.
DR KEGG; sce:YHR136C; -.
DR SGD; S000001178; SPL2.
DR VEuPathDB; FungiDB:YHR136C; -.
DR HOGENOM; CLU_2051493_0_0_1; -.
DR OMA; IWVFTES; -.
DR BioCyc; YEAST:G3O-31173-MON; -.
DR PRO; PR:P38839; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38839; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; ISS:SGD.
DR GO; GO:0006623; P:protein targeting to vacuole; IMP:SGD.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Protein kinase inhibitor; Reference proteome.
FT CHAIN 1..148
FT /note="Putative cyclin-dependent kinase inhibitor SPL2"
FT /id="PRO_0000202920"
FT MOD_RES 59
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 86
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956"
FT CONFLICT 58
FT /note="L -> P (in Ref. 1; CAA73949)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 148 AA; 17036 MW; 67C02AA833FB42E9 CRC64;
MGTYTPLIYN IYNVHIWVFT ESQGQIGQMS PRGKMETAVS QGQHKQLKDG HQHKGRKLSE
EIASLLRLKE CRRLNPASYY TPRRTSQSQS LSGSTFKEYN EYINEKDSSR AQRQNAAAVL
SKLAHDFWEN DCVIDEDIFE DSSDEEQS
