SPL3_ARATH
ID SPL3_ARATH Reviewed; 131 AA.
AC P93015; Q8LGE8; Q9LF84;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1999, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Squamosa promoter-binding-like protein 3;
GN Name=SPL3; OrderedLocusNames=At2g33810; ORFNames=T1B8.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=9301089; DOI=10.1046/j.1365-313x.1997.12020367.x;
RA Cardon G.H., Hoehmann S., Nettesheim K., Saedler H., Huijser P.;
RT "Functional analysis of the Arabidopsis thaliana SBP-box gene SPL3: a novel
RT gene involved in the floral transition.";
RL Plant J. 12:367-377(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=10524240; DOI=10.1016/s0378-1119(99)00308-x;
RA Cardon G.H., Hoehmann S., Klein J., Nettesheim K., Saedler H., Huijser P.;
RT "Molecular characterization of the Arabidopsis SBP-box genes.";
RL Gene 237:91-104(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP INDUCTION.
RX PubMed=12202040; DOI=10.1016/s0092-8674(02)00863-2;
RA Rhoades M.W., Reinhart B.J., Lim L.P., Burge C.B., Bartel B., Bartel D.P.;
RT "Prediction of plant microRNA targets.";
RL Cell 110:513-520(2002).
RN [9]
RP DEVELOPMENTAL STAGE.
RX PubMed=14573523; DOI=10.1242/dev.00842;
RA Schmid M., Uhlenhaut N.H., Godard F., Demar M., Bressan R., Weigel D.,
RA Lohmann J.U.;
RT "Dissection of floral induction pathways using global expression
RT analysis.";
RL Development 130:6001-6012(2003).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, REGION, AND
RP MUTAGENESIS OF ASP-108; ARG-112; SER-113 AND CYS-114.
RX PubMed=16095614; DOI=10.1016/j.jmb.2005.07.013;
RA Birkenbihl R.P., Jach G., Saedler H., Huijser P.;
RT "Functional dissection of the plant-specific SBP-domain: overlap of the
RT DNA-binding and nuclear localization domains.";
RL J. Mol. Biol. 352:585-596(2005).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=16914499; DOI=10.1242/dev.02521;
RA Wu G., Poethig R.S.;
RT "Temporal regulation of shoot development in Arabidopsis thaliana by miR156
RT and its target SPL3.";
RL Development 133:3539-3547(2006).
CC -!- FUNCTION: Trans-acting factor that binds specifically to the consensus
CC nucleotide sequence 5'-TNCGTACAA-3' of AP1 promoter. Binds specifically
CC to the 5'-GTAC-3' core sequence. Promotes both vegetative phase change
CC and flowering. Regulates phase-specific patterns of leaf epidermal
CC differentiation and flowering time, but does not seem to affect leaf
CC shape. {ECO:0000269|PubMed:16095614, ECO:0000269|PubMed:16914499,
CC ECO:0000269|PubMed:9301089}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16095614}. Cytoplasm
CC {ECO:0000269|PubMed:16095614}. Note=Mostly located in nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in vegetative and inflorescence apical
CC meristems, floral meristems, leaf and flower organ primordia,
CC inflorescence stem tissue and to lower extent in roots.
CC {ECO:0000269|PubMed:10524240, ECO:0000269|PubMed:9301089}.
CC -!- DEVELOPMENTAL STAGE: Increases during floral transition and stay high
CC thereafter. {ECO:0000269|PubMed:10524240, ECO:0000269|PubMed:14573523,
CC ECO:0000269|PubMed:16914499, ECO:0000269|PubMed:9301089}.
CC -!- INDUCTION: Negatively regulated by microRNAs miR156.
CC {ECO:0000269|PubMed:12202040, ECO:0000269|PubMed:16914499}.
CC -!- DOMAIN: The SBP-type zinc finger is required for the binding to DNA.
CC -!- DISRUPTION PHENOTYPE: Plants flower early and have a significantly
CC reduced number of juvenile, adult and cauline leaves, with a shorter
CC petiole and a more acute leaf base in the first two leaves.
CC {ECO:0000269|PubMed:16914499}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-3 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB94233.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y09427; CAA70578.1; -; mRNA.
DR EMBL; AJ011627; CAB56579.1; -; Genomic_DNA.
DR EMBL; AJ242959; CAB94233.1; ALT_INIT; mRNA.
DR EMBL; AJ011633; CAB56585.1; -; mRNA.
DR EMBL; U78721; AAC69133.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08889.1; -; Genomic_DNA.
DR EMBL; AK118179; BAC42802.1; -; mRNA.
DR EMBL; BT005443; AAO63863.1; -; mRNA.
DR EMBL; AY084312; AAM67271.1; -; mRNA.
DR PIR; H84749; H84749.
DR PIR; T52597; T52597.
DR RefSeq; NP_565771.1; NM_128940.2.
DR AlphaFoldDB; P93015; -.
DR SMR; P93015; -.
DR BioGRID; 3295; 1.
DR STRING; 3702.AT2G33810.1; -.
DR iPTMnet; P93015; -.
DR PaxDb; P93015; -.
DR PRIDE; P93015; -.
DR ProteomicsDB; 234096; -.
DR EnsemblPlants; AT2G33810.1; AT2G33810.1; AT2G33810.
DR GeneID; 817948; -.
DR Gramene; AT2G33810.1; AT2G33810.1; AT2G33810.
DR KEGG; ath:AT2G33810; -.
DR Araport; AT2G33810; -.
DR TAIR; locus:2057656; AT2G33810.
DR eggNOG; ENOG502RZTN; Eukaryota.
DR HOGENOM; CLU_065896_2_0_1; -.
DR InParanoid; P93015; -.
DR OMA; CTADMSK; -.
DR OrthoDB; 1525145at2759; -.
DR PhylomeDB; P93015; -.
DR PRO; PR:P93015; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P93015; differential.
DR Genevisible; P93015; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; TAS:TAIR.
DR GO; GO:0010229; P:inflorescence development; IMP:TAIR.
DR GO; GO:0009911; P:positive regulation of flower development; IMP:TAIR.
DR GO; GO:0010321; P:regulation of vegetative phase change; IMP:TAIR.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IMP:TAIR.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR017238; SBP_fam.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR PIRSF; PIRSF037575; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Differentiation; DNA-binding; Flowering;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..131
FT /note="Squamosa promoter-binding-like protein 3"
FT /id="PRO_0000132724"
FT ZN_FING 51..128
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 45..129
FT /note="Sufficient and necessary for DNA binding"
FT MOTIF 111..127
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 1..18
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..36
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 54
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 59
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 76
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 79
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 95
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 98
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 102
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 114
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT MUTAGEN 108
FT /note="D->A: Slight decrease in DNA binding efficiency."
FT /evidence="ECO:0000269|PubMed:16095614"
FT MUTAGEN 112
FT /note="R->Q: Slight decrease in DNA binding efficiency."
FT /evidence="ECO:0000269|PubMed:16095614"
FT MUTAGEN 113
FT /note="S->A: Slight decrease in DNA binding efficiency."
FT /evidence="ECO:0000269|PubMed:16095614"
FT MUTAGEN 113
FT /note="S->D: Complete loss of DNA binding."
FT /evidence="ECO:0000269|PubMed:16095614"
FT MUTAGEN 114
FT /note="C->A: Increase in nuclear import efficiency."
FT /evidence="ECO:0000269|PubMed:16095614"
FT CONFLICT 63
FT /note="M -> I (in Ref. 7; AAM67271)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 131 AA; 15304 MW; F3B4C7D8F6537501 CRC64;
MSMRRSKAEG KRSLRELSEE EEEEEETEDE DTFEEEEALE KKQKGKATSS SGVCQVESCT
ADMSKAKQYH KRHKVCQFHA KAPHVRISGL HQRFCQQCSR FHALSEFDEA KRSCRRRLAG
HNERRRKSTT D