SPL6_ARATH
ID SPL6_ARATH Reviewed; 405 AA.
AC Q94JW8; Q0WSG7; Q9LQA4; Q9S7A2;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Squamosa promoter-binding-like protein 6;
GN Name=SPL6; OrderedLocusNames=At1g69170; ORFNames=F23O10.24, F4N2.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=10524240; DOI=10.1016/s0378-1119(99)00308-x;
RA Cardon G.H., Hoehmann S., Klein J., Nettesheim K., Saedler H., Huijser P.;
RT "Molecular characterization of the Arabidopsis SBP-box genes.";
RL Gene 237:91-104(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION.
RX PubMed=12202040; DOI=10.1016/s0092-8674(02)00863-2;
RA Rhoades M.W., Reinhart B.J., Lim L.P., Burge C.B., Bartel B., Bartel D.P.;
RT "Prediction of plant microRNA targets.";
RL Cell 110:513-520(2002).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=14573523; DOI=10.1242/dev.00842;
RA Schmid M., Uhlenhaut N.H., Godard F., Demar M., Bressan R., Weigel D.,
RA Lohmann J.U.;
RT "Dissection of floral induction pathways using global expression
RT analysis.";
RL Development 130:6001-6012(2003).
CC -!- FUNCTION: Trans-acting factor that binds specifically to the consensus
CC nucleotide sequence 5'-TNCGTACAA-3'. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- INTERACTION:
CC Q94JW8; O82277: TCP10; NbExp=3; IntAct=EBI-4428552, EBI-3133327;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed constitutively during plant development,
CC weak increase during flowering. {ECO:0000269|PubMed:10524240,
CC ECO:0000269|PubMed:14573523}.
CC -!- INDUCTION: Negatively regulated by microRNAs miR156 and miR157.
CC {ECO:0000305|PubMed:12202040}.
CC -!- DOMAIN: The SBP-type zinc finger is required for the binding to DNA.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF27058.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ011643; CAB56595.1; -; mRNA.
DR EMBL; AJ011644; CAB56596.1; -; Genomic_DNA.
DR EMBL; AC008262; AAF27058.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC018364; AAG52487.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34890.1; -; Genomic_DNA.
DR EMBL; AF370612; AAK43931.1; -; mRNA.
DR EMBL; BT006436; AAP21244.1; -; mRNA.
DR EMBL; AK227964; BAE99931.1; -; mRNA.
DR PIR; T52592; T52592.
DR RefSeq; NP_177077.3; NM_105584.6.
DR AlphaFoldDB; Q94JW8; -.
DR SMR; Q94JW8; -.
DR BioGRID; 28469; 28.
DR IntAct; Q94JW8; 27.
DR STRING; 3702.AT1G69170.1; -.
DR PaxDb; Q94JW8; -.
DR PRIDE; Q94JW8; -.
DR EnsemblPlants; AT1G69170.1; AT1G69170.1; AT1G69170.
DR GeneID; 843248; -.
DR Gramene; AT1G69170.1; AT1G69170.1; AT1G69170.
DR KEGG; ath:AT1G69170; -.
DR Araport; AT1G69170; -.
DR TAIR; locus:2026428; AT1G69170.
DR eggNOG; ENOG502QUHN; Eukaryota.
DR HOGENOM; CLU_835095_0_0_1; -.
DR InParanoid; Q94JW8; -.
DR OMA; MDEHDHR; -.
DR OrthoDB; 975062at2759; -.
DR PhylomeDB; Q94JW8; -.
DR PRO; PR:Q94JW8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94JW8; baseline and differential.
DR Genevisible; Q94JW8; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0010468; P:regulation of gene expression; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..405
FT /note="Squamosa promoter-binding-like protein 6"
FT /id="PRO_0000132727"
FT ZN_FING 121..198
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT MOTIF 181..197
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT BINDING 124
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 129
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 146
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 149
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 165
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 168
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 172
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 184
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
SQ SEQUENCE 405 AA; 45953 MW; B7DD1356C6DEE259 CRC64;
MDSWSYGRSV FMSNETLLPC DTFAKNRRFE QRLSNNDDVL ISDMAGNSNG FSAVSITKVV
PEEEDEENIS SSSKFSSQEL NRIDFKLRSF LDLGNDDDDT SSRGFALPSK KSRASNLCSQ
NPLCQVYGCS KDLSSSKDYH KRHRVCEAHS KTSVVIVNGL EQRFCQQCSR FHFLSEFDDG
KRSCRRRLAG HNERRRKPAF YFLPGKRHKL LRTSQDVVGN KFLENSSLVL PESFPGSLLY
RVIDEDDHRT SRLVSFKDEP TCSMFPTNEQ NSSRTYESKP AIYSTEVSSI WDLHETAASR
STRALSLLSA QSQQHLSKFP NTTFSITQPN QNLNHSSSTD YHQMEQPLWI DPGKTNSAGS
SSCKGKGTST VDLLQLSSHL QRIEQQRNYT GDVKQEYNEL YFPGS
