SPL8_ARATH
ID SPL8_ARATH Reviewed; 333 AA.
AC Q8GXL3; Q9S849;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Squamosa promoter-binding-like protein 8;
GN Name=SPL8; OrderedLocusNames=At1g02065; ORFNames=T7I23.32;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=10524240; DOI=10.1016/s0378-1119(99)00308-x;
RA Cardon G.H., Hoehmann S., Klein J., Nettesheim K., Saedler H., Huijser P.;
RT "Molecular characterization of the Arabidopsis SBP-box genes.";
RL Gene 237:91-104(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=12671094; DOI=10.1105/tpc.010678;
RA Unte U.S., Sorensen A.-M., Pesaresi P., Gandikota M., Leister D.,
RA Saedler H., Huijser P.;
RT "SPL8, an SBP-box gene that affects pollen sac development in
RT Arabidopsis.";
RL Plant Cell 15:1009-1019(2003).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, REGION, AND
RP MUTAGENESIS OF SER-233; SER-247 AND CYS-248.
RX PubMed=16095614; DOI=10.1016/j.jmb.2005.07.013;
RA Birkenbihl R.P., Jach G., Saedler H., Huijser P.;
RT "Functional dissection of the plant-specific SBP-domain: overlap of the
RT DNA-binding and nuclear localization domains.";
RL J. Mol. Biol. 352:585-596(2005).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=17093870; DOI=10.1007/s11103-006-9099-6;
RA Zhang Y., Schwarz S., Saedler H., Huijser P.;
RT "SPL8, a local regulator in a subset of gibberellin-mediated developmental
RT processes in Arabidopsis.";
RL Plant Mol. Biol. 63:429-439(2007).
CC -!- FUNCTION: Trans-acting factor that binds specifically to the consensus
CC nucleotide sequence 5'-TNCGTACAA-3'. Binds specifically to the 5'-GTAC-
CC 3' core sequence. Involved in development and floral organogenesis.
CC Required for ovule differentiation, pollen production, filament
CC elongation, seed formation and siliques elongation. Also seems to play
CC a role in the formation of trichomes on sepals. May positively modulate
CC gibberellin (GA) signaling in flower. {ECO:0000269|PubMed:12671094,
CC ECO:0000269|PubMed:16095614, ECO:0000269|PubMed:17093870}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16095614}. Cytoplasm
CC {ECO:0000269|PubMed:16095614}. Note=Mostly located in nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8GXL3-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in shoot apical region and early floral
CC tissues. Transcripts levels increase in developing pollen sacs, and
CC decrease in later stage of anther development. Strongly expressed in
CC the placental region of the carpels. {ECO:0000269|PubMed:12671094,
CC ECO:0000269|PubMed:17093870}.
CC -!- DEVELOPMENTAL STAGE: Expressed during plant development. During pollen
CC development, required for crosporogenesis that leads to archesporial
CC formation and for the histogenesis of the microsporangia. During ovules
CC development, involved in the transition to meiosis of the megaspore
CC mother cells. {ECO:0000269|PubMed:10524240,
CC ECO:0000269|PubMed:12671094}.
CC -!- DOMAIN: The SBP-type zinc finger is required for the binding to DNA.
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DR EMBL; AJ011641; CAB56593.1; -; Genomic_DNA.
DR EMBL; AJ011642; CAB56594.1; -; mRNA.
DR EMBL; U89959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE27374.1; -; Genomic_DNA.
DR EMBL; AK118174; BAC42797.1; -; mRNA.
DR PIR; T52594; T52594.
DR RefSeq; NP_683267.1; NM_148426.5. [Q8GXL3-1]
DR AlphaFoldDB; Q8GXL3; -.
DR SMR; Q8GXL3; -.
DR BioGRID; 24510; 3.
DR STRING; 3702.AT1G02065.1; -.
DR iPTMnet; Q8GXL3; -.
DR PaxDb; Q8GXL3; -.
DR PRIDE; Q8GXL3; -.
DR ProteomicsDB; 232489; -. [Q8GXL3-1]
DR EnsemblPlants; AT1G02065.1; AT1G02065.1; AT1G02065. [Q8GXL3-1]
DR GeneID; 839275; -.
DR Gramene; AT1G02065.1; AT1G02065.1; AT1G02065. [Q8GXL3-1]
DR KEGG; ath:AT1G02065; -.
DR Araport; AT1G02065; -.
DR TAIR; locus:504956101; AT1G02065.
DR eggNOG; ENOG502QWHY; Eukaryota.
DR HOGENOM; CLU_082879_0_0_1; -.
DR InParanoid; Q8GXL3; -.
DR OMA; DPRITNH; -.
DR PhylomeDB; Q8GXL3; -.
DR PRO; PR:Q8GXL3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GXL3; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:TAIR.
DR GO; GO:0003677; F:DNA binding; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0048653; P:anther development; IGI:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009554; P:megasporogenesis; IMP:TAIR.
DR GO; GO:0009556; P:microsporogenesis; IMP:TAIR.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW DNA-binding; Flowering; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..333
FT /note="Squamosa promoter-binding-like protein 8"
FT /id="PRO_0000132729"
FT ZN_FING 185..262
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 179..269
FT /note="Sufficient and necessary for DNA binding"
FT REGION 254..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 314..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 245..261
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 266..303
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 188
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 193
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 210
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 213
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 229
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 232
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 236
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 248
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT MUTAGEN 233
FT /note="S->A: Slight decrease in DNA binding efficiency."
FT /evidence="ECO:0000269|PubMed:16095614"
FT MUTAGEN 247
FT /note="S->A: Slight decrease in DNA binding and nuclear
FT import efficiency."
FT /evidence="ECO:0000269|PubMed:16095614"
FT MUTAGEN 247
FT /note="S->D: Complete loss of DNA binding. Slight increase
FT in nuclear import efficiency."
FT /evidence="ECO:0000269|PubMed:16095614"
FT MUTAGEN 248
FT /note="C->A: Increase in nuclear import efficiency."
FT /evidence="ECO:0000269|PubMed:16095614"
FT CONFLICT 63
FT /note="H -> Q (in Ref. 4; BAC42797)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="Q -> R (in Ref. 4; BAC42797)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 333 AA; 36827 MW; 0182715A7A35D394 CRC64;
MLDYEWDNPS SIVLSGDERN PDSDPTRSSF SFFDPISHYN NDHRHITISP PLLSSFSNQQ
QQHHLTLYGQ TNSNNQFLHH HHHHHSLYGS TTTTTPYGAS DPIYHPHSSA PPASLFSYDQ
TGPGSGSGSS YNFLIPKTEV DFTSNRIGLN LGGRTYFSAA DDDFVSRLYR RSRPGESGMA
NSLSTPRCQA EGCNADLSHA KHYHRRHKVC EFHSKASTVV AAGLSQRFCQ QCSRFHLLSE
FDNGKRSCRK RLADHNRRRR KCHQSASATQ DTGTGKTTPK SPNDSGVKAS SSPSSNAPPT
ISLECFRQRQ FQTTASSSTS ASSSSNSMFF SSG