SPL8_ORYSJ
ID SPL8_ORYSJ Reviewed; 416 AA.
AC Q7XPY1; A0A0P0WFS6;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Squamosa promoter-binding-like protein 8;
DE AltName: Full=OsLG1;
DE AltName: Full=Protein LIGULELESS 1;
GN Name=SPL8; Synonyms=LG1; OrderedLocusNames=Os04g0656500, LOC_Os04g56170;
GN ORFNames=OSJNBa0071I13.12;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16861571; DOI=10.1104/pp.106.084475;
RA Xie K., Wu C., Xiong L.;
RT "Genomic organization, differential expression, and interaction of SQUAMOSA
RT promoter-binding-like transcription factors and microRNA156 in rice.";
RL Plant Physiol. 142:280-293(2006).
RN [7]
RP GENE FAMILY.
RX PubMed=17629421; DOI=10.1016/j.gene.2007.02.034;
RA Yang Z., Wang X., Gu S., Hu Z., Xu H., Xu C.;
RT "Comparative study of SBP-box gene family in Arabidopsis and rice.";
RL Gene 407:1-11(2008).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=17594063; DOI=10.1007/s11103-007-9196-1;
RA Lee J., Park J.-J., Kim S.L., Yim J., An G.;
RT "Mutations in the rice liguleless gene result in a complete loss of the
RT auricle, ligule, and laminar joint.";
RL Plant Mol. Biol. 65:487-499(2007).
CC -!- FUNCTION: Probable transcription factor that plays an important role in
CC building the laminar joint between leaf blade and leaf sheath boundary,
CC thereby controlling ligule and auricle development.
CC {ECO:0000269|PubMed:17594063}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17594063}.
CC -!- TISSUE SPECIFICITY: Expressed in stems, leaf sheaths, and young
CC panicles. Weakly expressed in ligules, auricles, and leaf sheaths at
CC the basal region. {ECO:0000269|PubMed:16861571,
CC ECO:0000269|PubMed:17594063}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing laminar joint and
CC meristematic regions.
CC -!- DOMAIN: The SBP-type zinc finger is required for the binding to DNA.
CC {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Plants show upright attitudes and the absence of
CC laminar joints, ligules and auricules in all the leaves including the
CC flag leaves. {ECO:0000269|PubMed:17594063}.
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DR EMBL; AL606685; CAE03411.3; -; Genomic_DNA.
DR EMBL; AP008210; BAF16033.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS91410.1; -; Genomic_DNA.
DR EMBL; AK068104; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015634037.1; XM_015778551.1.
DR AlphaFoldDB; Q7XPY1; -.
DR SMR; Q7XPY1; -.
DR STRING; 4530.OS04T0656500-01; -.
DR PaxDb; Q7XPY1; -.
DR PRIDE; Q7XPY1; -.
DR EnsemblPlants; Os04t0656500-01; Os04t0656500-01; Os04g0656500.
DR GeneID; 4337258; -.
DR Gramene; Os04t0656500-01; Os04t0656500-01; Os04g0656500.
DR KEGG; osa:4337258; -.
DR eggNOG; ENOG502QWHY; Eukaryota.
DR HOGENOM; CLU_689660_0_0_1; -.
DR InParanoid; Q7XPY1; -.
DR OMA; FYPTSHY; -.
DR OrthoDB; 934410at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR Genevisible; Q7XPY1; OS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..416
FT /note="Squamosa promoter-binding-like protein 8"
FT /id="PRO_0000308232"
FT ZN_FING 182..260
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT REGION 11..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 243..259
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 257..279
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 185
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 190
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 207
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 210
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 227
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 230
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 234
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 246
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT CONFLICT 47
FT /note="Q -> H (in Ref. 5; AK068104)"
FT /evidence="ECO:0000305"
FT CONFLICT 135
FT /note="D -> A (in Ref. 5; AK068104)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 416 AA; 45030 MW; C914F8CE433A8A9F CRC64;
MMNVPSAAAA SSCDDFGYNA TPPPPPSLLP IMDQDGGGGS IQRDHHQHHN HQQLGYNLEP
SSLALLPPSN AAAAAAHHAT IAHASPHDLL QFYPTSHYLA AAGGAGGGGN PYSHFTAAAA
AGSTFQSYYQ QPPQDAPEYY FPTLVSSAEE NMASFAATQL GLNLGYRTYF PPRGGYTYGH
HPPRCQAEGC KADLSSAKRY HRRHKVCEHH SKAPVVVTAG GLHQRFCQQC SRFHLLDEFD
DAKKSCRKRL ADHNRRRRKS KPSDGEHSGE KRRAQANKSA ATKDKAGSSS KNAGIGDGFE
TQLLGGAHMS KDQDQAMDLG EVVKEAVDPK GKASMQQQQQ QAHHGIHQQS HQQHGFPFPS
SSGSCLFPQS QGAVSSTDTS NIAQVQEPSL AFHQQHHQHS NILQLGQAMF DLDFDH
