SPL9_ARATH
ID SPL9_ARATH Reviewed; 375 AA.
AC Q700W2; O48527; Q94AJ2; Q9SMX7;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Squamosa promoter-binding-like protein 9;
GN Name=SPL9; OrderedLocusNames=At2g42200; ORFNames=T24P15.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=10524240; DOI=10.1016/s0378-1119(99)00308-x;
RA Cardon G.H., Hoehmann S., Klein J., Nettesheim K., Saedler H., Huijser P.;
RT "Molecular characterization of the Arabidopsis SBP-box genes.";
RL Gene 237:91-104(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=15208423; DOI=10.1104/pp.104.042176;
RA Gong W., Shen Y.-P., Ma L.-G., Pan Y., Du Y.-L., Wang D.-H., Yang J.-Y.,
RA Hu L.-D., Liu X.-F., Dong C.-X., Ma L., Chen Y.-H., Yang X.-Y., Gao Y.,
RA Zhu D., Tan X., Mu J.-Y., Zhang D.-B., Liu Y.-L., Dinesh-Kumar S.P., Li Y.,
RA Wang X.-P., Gu H.-Y., Qu L.-J., Bai S.-N., Lu Y.-T., Li J.-Y., Zhao J.-D.,
RA Zuo J., Huang H., Deng X.-W., Zhu Y.-X.;
RT "Genome-wide ORFeome cloning and analysis of Arabidopsis transcription
RT factor genes.";
RL Plant Physiol. 135:773-782(2004).
RN [6]
RP INDUCTION.
RX PubMed=12202040; DOI=10.1016/s0092-8674(02)00863-2;
RA Rhoades M.W., Reinhart B.J., Lim L.P., Burge C.B., Bartel B., Bartel D.P.;
RT "Prediction of plant microRNA targets.";
RL Cell 110:513-520(2002).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=14573523; DOI=10.1242/dev.00842;
RA Schmid M., Uhlenhaut N.H., Godard F., Demar M., Bressan R., Weigel D.,
RA Lohmann J.U.;
RT "Dissection of floral induction pathways using global expression
RT analysis.";
RL Development 130:6001-6012(2003).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=16095614; DOI=10.1016/j.jmb.2005.07.013;
RA Birkenbihl R.P., Jach G., Saedler H., Huijser P.;
RT "Functional dissection of the plant-specific SBP-domain: overlap of the
RT DNA-binding and nuclear localization domains.";
RL J. Mol. Biol. 352:585-596(2005).
CC -!- FUNCTION: Trans-acting factor that binds specifically to the consensus
CC nucleotide sequence 5'-TNCGTACAA-3'. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16095614}. Cytoplasm
CC {ECO:0000269|PubMed:16095614}. Note=Mostly located in nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q700W2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q700W2-2; Sequence=VSP_013985;
CC -!- DEVELOPMENTAL STAGE: Expressed constitutively during plant development,
CC with a strong increase during flower development.
CC {ECO:0000269|PubMed:10524240, ECO:0000269|PubMed:14573523}.
CC -!- INDUCTION: Negatively regulated by microRNAs miR156 and miR157.
CC {ECO:0000305|PubMed:12202040}.
CC -!- DOMAIN: The SBP-type zinc finger is required for the binding to DNA.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ011638; CAB56590.1; -; mRNA.
DR EMBL; AJ011639; CAB56591.1; -; mRNA.
DR EMBL; AJ011640; CAB56592.1; -; Genomic_DNA.
DR EMBL; AC002561; AAB88645.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10085.1; -; Genomic_DNA.
DR EMBL; AY046007; AAK76681.1; -; mRNA.
DR EMBL; AY150378; AAN12923.1; -; mRNA.
DR EMBL; AJ628864; CAG25585.1; -; mRNA.
DR PIR; T00929; T00929.
DR PIR; T52593; T52593.
DR RefSeq; NP_181749.1; NM_129782.3. [Q700W2-1]
DR AlphaFoldDB; Q700W2; -.
DR SMR; Q700W2; -.
DR STRING; 3702.AT2G42200.1; -.
DR iPTMnet; Q700W2; -.
DR PaxDb; Q700W2; -.
DR PRIDE; Q700W2; -.
DR ProteomicsDB; 245197; -. [Q700W2-1]
DR EnsemblPlants; AT2G42200.1; AT2G42200.1; AT2G42200. [Q700W2-1]
DR GeneID; 818820; -.
DR Gramene; AT2G42200.1; AT2G42200.1; AT2G42200. [Q700W2-1]
DR KEGG; ath:AT2G42200; -.
DR Araport; AT2G42200; -.
DR TAIR; locus:2059974; AT2G42200.
DR eggNOG; ENOG502QPVZ; Eukaryota.
DR HOGENOM; CLU_057950_1_0_1; -.
DR InParanoid; Q700W2; -.
DR OMA; SPSWQIN; -.
DR OrthoDB; 910635at2759; -.
DR PhylomeDB; Q700W2; -.
DR PRO; PR:Q700W2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q700W2; baseline and differential.
DR Genevisible; Q700W2; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0048653; P:anther development; IGI:TAIR.
DR GO; GO:0048366; P:leaf development; IDA:TAIR.
DR GO; GO:2000025; P:regulation of leaf formation; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..375
FT /note="Squamosa promoter-binding-like protein 9"
FT /id="PRO_0000132730"
FT ZN_FING 71..148
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 43..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 252..278
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 345..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 131..147
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 16..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 345..365
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 74
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 79
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 96
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 99
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 115
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 118
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 122
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 134
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT VAR_SEQ 324..331
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10524240"
FT /id="VSP_013985"
FT CONFLICT 243
FT /note="I -> N (in Ref. 4; AAK76681)"
FT /evidence="ECO:0000305"
FT CONFLICT 262
FT /note="Missing (in Ref. 4; AAK76681)"
FT /evidence="ECO:0000305"
FT CONFLICT 275
FT /note="N -> NNNN (in Ref. 5; CAG25585)"
FT /evidence="ECO:0000305"
FT CONFLICT 275
FT /note="N -> NNNNNNN (in Ref. 1; CAB56591)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 375 AA; 40847 MW; D1B93F98D70ECD28 CRC64;
MEMGSNSGPG HGPGQAESGG SSTESSSFSG GLMFGQKIYF EDGGGGSGSS SSGGRSNRRV
RGGGSGQSGQ IPRCQVEGCG MDLTNAKGYY SRHRVCGVHS KTPKVTVAGI EQRFCQQCSR
FHQLPEFDLE KRSCRRRLAG HNERRRKPQP ASLSVLASRY GRIAPSLYEN GDAGMNGSFL
GNQEIGWPSS RTLDTRVMRR PVSSPSWQIN PMNVFSQGSV GGGGTSFSSP EIMDTKLESY
KGIGDSNCAL SLLSNPHQPH DNNNNNNNNN NNNNNTWRAS SGFGPMTVTM AQPPPAPSQH
QYLNPPWVFK DNDNDMSPVL NLGRYTEPDN CQISSGTAMG EFELSDHHHQ SRRQYMEDEN
TRAYDSSSHH TNWSL
