ID   SPLA1_STAAU             Reviewed;         235 AA.
AC   Q9FD08;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Serine protease SplA;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=splA;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=FRI326;
RA   Nair S.P., Williams R.J.;
RT   "Cloning and expression of two genes from a novel Staphylococcus aureus
RT   operon encoding serine protease like exoproteins.";
RL   Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S1B family. {ECO:0000305}.
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DR   EMBL; AF295600; AAG02238.1; -; Genomic_DNA.
DR   RefSeq; WP_001039421.1; NZ_LS483302.1.
DR   AlphaFoldDB; Q9FD08; -.
DR   SMR; Q9FD08; -.
DR   MEROPS; S01.503; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR008256; Peptidase_S1B.
DR   InterPro; IPR008353; Peptidase_S1B_tx.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR028301; V8_his_AS.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR01774; EXFOLTOXIN.
DR   PRINTS; PR00839; V8PROTEASE.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS00672; V8_HIS; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Secreted; Serine protease; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000250"
FT   CHAIN           36..235
FT                   /note="Serine protease SplA"
FT                   /id="PRO_0000359525"
FT   ACT_SITE        74
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        113
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        189
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   235 AA;  25514 MW;  6DE1C607B559C96B CRC64;
     MNKNVMIKGL TALTILTSLG FAENISDQPH SIAKAEKNIK EVTDATKAPY NSVVAFAGGT
     GVVVGKNTIV TNKHIAKSND IFKNRVSAHH SSKGKGGGNY DVKDIVEYPG KEDLAIVHVH
     ETSTEGLNFN KNVSYTKFAE GAKMKDRISV IGYPKGAQTK YKMFESTGTI NHINGTFMEF
     DAYAQPGNSG SPVLNSKNEL VGILYAGSGK DESEKNFGVY FTPQLKEFIQ NNIEK