ID   SPLA2_STAAU             Reviewed;         235 AA.
AC   A5JJ15; A5JJ16; A5JJ17;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2009, sequence version 2.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Serine protease SplA;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=splA;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=VBI-159227, VBI-160099, and VBI-M0894;
RX   PubMed=17614219; DOI=10.1016/j.vetmic.2007.05.016;
RA   Monecke S., Kuhnert P., Hotzel H., Slickers P., Ehricht R.;
RT   "Microarray based study on virulence-associated genes and resistance
RT   determinants of Staphylococcus aureus isolates from cattle.";
RL   Vet. Microbiol. 125:128-140(2007).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S1B family. {ECO:0000305}.
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DR   EMBL; EF531611; ABQ44276.1; -; Genomic_DNA.
DR   EMBL; EF531612; ABQ44277.1; -; Genomic_DNA.
DR   EMBL; EF531613; ABQ44278.1; -; Genomic_DNA.
DR   AlphaFoldDB; A5JJ15; -.
DR   SMR; A5JJ15; -.
DR   MEROPS; S01.503; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR008256; Peptidase_S1B.
DR   InterPro; IPR008353; Peptidase_S1B_tx.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR028301; V8_his_AS.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR01774; EXFOLTOXIN.
DR   PRINTS; PR00839; V8PROTEASE.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS00672; V8_HIS; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Secreted; Serine protease; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000250"
FT   CHAIN           36..235
FT                   /note="Serine protease SplA"
FT                   /id="PRO_0000359526"
FT   ACT_SITE        74
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        113
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        189
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   VARIANT         14
FT                   /note="D -> T (in strain: VBI-M0894)"
FT   VARIANT         21
FT                   /note="C -> F (in strain: VBI-M0894)"
FT   VARIANT         92
FT                   /note="S -> G (in strain: VBI-M0894)"
FT   VARIANT         124
FT                   /note="T -> I (in strain: VBI-M0894)"
FT   VARIANT         150
FT                   /note="I -> V (in strain: VBI-M0894)"
FT   VARIANT         157
FT                   /note="A -> S (in strain: VBI-M0894)"
FT   VARIANT         177
FT                   /note="I -> F (in strain: VBI-M0894)"
FT   VARIANT         217
FT                   /note="F -> Y (in strain: VBI-M0894)"
SQ   SEQUENCE   235 AA;  25389 MW;  82117B3FBA05D6BE CRC64;
     MNKNVMVKGL TALDILTSLG CAENISDQPH SIAKAEKNVK EITDATKAPY NSVVAFAGGT
     GVVVGKNTIV TNKHIAKSNN IFKNRVSAHH SSKGKGGGNY DVKDIVEYPG KEDLAIVHVH
     ETSTEGLNFN KNVSYTKFAD GAKAKDRISI IGYPKGAQTK YKMFESTGTI NHINGTIMEF
     DAYAQPGNSG SPVLNSKNEL IGILYAGSGK DESEKNFGVY FTPQLKEFIQ NNIEK