SPLA_STAA8
ID SPLA_STAA8 Reviewed; 235 AA.
AC Q2FXC2; Q9KH51;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Serine protease SplA;
DE EC=3.4.21.-;
DE Flags: Precursor;
GN Name=splA; OrderedLocusNames=SAOUHSC_01942;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-50, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=11179322; DOI=10.1128/iai.69.3.1521-1527.2001;
RA Reed S.B., Wesson C.A., Liou L.E., Trumble W.R., Schlievert P.M.,
RA Bohach G.A., Bayles K.W.;
RT "Molecular characterization of a novel Staphylococcus aureus serine
RT protease operon.";
RL Infect. Immun. 69:1521-1527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11179322}.
CC -!- DEVELOPMENTAL STAGE: Maximally expressed during early stationary phase.
CC {ECO:0000269|PubMed:11179322}.
CC -!- INDUCTION: Positively regulated by agr (accessory gene regulator).
CC {ECO:0000269|PubMed:11179322}.
CC -!- SIMILARITY: Belongs to the peptidase S1B family. {ECO:0000305}.
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DR EMBL; AF271715; AAF97925.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD31004.1; -; Genomic_DNA.
DR RefSeq; WP_001039435.1; NZ_LS483365.1.
DR RefSeq; YP_500442.1; NC_007795.1.
DR PDB; 2W7S; X-ray; 1.80 A; A/B/C/D=36-235.
DR PDB; 2W7U; X-ray; 2.43 A; A/B/C/D=36-235.
DR PDB; 3UFA; X-ray; 1.80 A; A/B=36-235.
DR PDB; 4MVN; X-ray; 1.70 A; A/B/C/D=36-235.
DR PDBsum; 2W7S; -.
DR PDBsum; 2W7U; -.
DR PDBsum; 3UFA; -.
DR PDBsum; 4MVN; -.
DR AlphaFoldDB; Q2FXC2; -.
DR SMR; Q2FXC2; -.
DR STRING; 1280.SAXN108_1846; -.
DR MEROPS; S01.503; -.
DR EnsemblBacteria; ABD31004; ABD31004; SAOUHSC_01942.
DR GeneID; 3921025; -.
DR KEGG; sao:SAOUHSC_01942; -.
DR PATRIC; fig|93061.5.peg.1767; -.
DR eggNOG; COG3591; Bacteria.
DR HOGENOM; CLU_073589_2_0_9; -.
DR OMA; NAQFEVV; -.
DR EvolutionaryTrace; Q2FXC2; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR008256; Peptidase_S1B.
DR InterPro; IPR008353; Peptidase_S1B_tx.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR028301; V8_his_AS.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR01774; EXFOLTOXIN.
DR PRINTS; PR00839; V8PROTEASE.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS00672; V8_HIS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000269|PubMed:11179322"
FT CHAIN 36..235
FT /note="Serine protease SplA"
FT /id="PRO_0000359533"
FT ACT_SITE 74
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 113
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 189
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT STRAND 39..43
FT /evidence="ECO:0007829|PDB:4MVN"
FT HELIX 50..52
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 53..56
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 59..65
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:4MVN"
FT HELIX 73..82
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 86..89
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 92..94
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 102..107
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 109..113
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 115..119
FT /evidence="ECO:0007829|PDB:4MVN"
FT HELIX 129..132
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 147..152
FT /evidence="ECO:0007829|PDB:4MVN"
FT HELIX 156..158
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 163..174
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 177..180
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 192..194
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 200..207
FT /evidence="ECO:0007829|PDB:4MVN"
FT STRAND 215..220
FT /evidence="ECO:0007829|PDB:4MVN"
FT HELIX 223..231
FT /evidence="ECO:0007829|PDB:4MVN"
SQ SEQUENCE 235 AA; 25549 MW; DBC6B23C6648BCF9 CRC64;
MNKNVMVKGL TALTILTSLG FAENISNQPH SIAKAEKNVK EITDATKEPY NSVVAFVGGT
GVVVGKNTIV TNKHIAKSND IFKNRVSAHH SSKGKGGGNY DVKDIVEYPG KEDLAIVHVH
ETSTEGLNFN KNVSYTKFAD GAKVKDRISV IGYPKGAQTK YKMFESTGTI NHISGTFMEF
DAYAQPGNSG SPVLNSKHEL IGILYAGSGK DESEKNFGVY FTPQLKEFIQ NNIEK
