SPLB_DICDI
ID SPLB_DICDI Reviewed; 1155 AA.
AC P18161; Q54NC7;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Dual specificity protein kinase splB;
DE EC=2.7.10.2;
DE AltName: Full=Non-receptor tyrosine kinase spore lysis B;
DE AltName: Full=Tyrosine-protein kinase 2;
GN Name=splB; Synonyms=DpyK2, pyk2, pykB; ORFNames=DDB_G0285321;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 746-1155.
RX PubMed=1972546; DOI=10.1128/mcb.10.7.3578-3583.1990;
RA Tan J.L., Spudich J.A.;
RT "Developmentally regulated protein-tyrosine kinase genes in Dictyostelium
RT discoideum.";
RL Mol. Cell. Biol. 10:3578-3583(1990).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.2;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10028};
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. TKL Tyr protein
CC kinase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAFI02000079; EAL64735.1; -; Genomic_DNA.
DR EMBL; M33784; AAA33203.1; -; mRNA.
DR PIR; B35670; B35670.
DR RefSeq; XP_638253.1; XM_633161.1.
DR AlphaFoldDB; P18161; -.
DR SMR; P18161; -.
DR STRING; 44689.DDB0191483; -.
DR PaxDb; P18161; -.
DR EnsemblProtists; EAL64735; EAL64735; DDB_G0285321.
DR GeneID; 8625061; -.
DR KEGG; ddi:DDB_G0285321; -.
DR dictyBase; DDB_G0285321; splB.
DR eggNOG; KOG0192; Eukaryota.
DR HOGENOM; CLU_276010_0_0_1; -.
DR InParanoid; P18161; -.
DR OMA; MTEVCNE; -.
DR PRO; PR:P18161; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0004672; F:protein kinase activity; IBA:GO_Central.
DR GO; GO:0004713; F:protein tyrosine kinase activity; IDA:dictyBase.
DR GO; GO:0006972; P:hyperosmotic response; IGI:dictyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IGI:dictyBase.
DR GO; GO:0042307; P:positive regulation of protein import into nucleus; IMP:dictyBase.
DR GO; GO:1904894; P:positive regulation of receptor signaling pathway via STAT; IGI:dictyBase.
DR GO; GO:0046777; P:protein autophosphorylation; IDA:dictyBase.
DR GO; GO:1903013; P:response to differentiation-inducing factor 1; IMP:dictyBase.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0030587; P:sorocarp development; IGI:dictyBase.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR PRINTS; PR00109; TYRKINASE.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Lipoprotein; Myristate; Nucleotide-binding;
KW Reference proteome; Transferase; Tyrosine-protein kinase.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..1155
FT /note="Dual specificity protein kinase splB"
FT /id="PRO_0000088122"
FT DOMAIN 853..1126
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 352..423
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 453..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 571..599
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 710..753
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1134..1155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..25
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 77..215
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 226..253
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..381
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..423
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 977
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10028"
FT BINDING 859..867
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 880
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT CONFLICT 746..749
FT /note="NSTS -> RFYN (in Ref. 2; AAA33203)"
FT /evidence="ECO:0000305"
FT CONFLICT 1000
FT /note="R -> A (in Ref. 2; AAA33203)"
FT /evidence="ECO:0000305"
FT CONFLICT 1063
FT /note="A -> R (in Ref. 2; AAA33203)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1155 AA; 127437 MW; 2A00D04A39262BC2 CRC64;
MGITSSKPVS GSNTTTNSPT NQKAISEKKQ KPKRTNKRNL STDSKSSTCS SPTNNYLADE
LVVDLNSMEI EGADSIQYSG HSHSSSMTNS MTNSMRNSAN EKQLQQKQQQ PQQQPQQQQP
QPHQSHDDLN SSALDQDDGE FNNNFENESE VESISSSSIN NNNTNNNSNN NNNNNNNNNN
NNNNNNNIPS ISNISNNNIN NNINNNNNNN SCKQKNSKNK KNKNKNNNNK NSSSSNERPY
SISYDNEGST TKSNSTLSLY SNISNLHSIT SHSIQDASIV CENCNHHMTK PIHLLSLSLE
PTSAASLDIH RNGNFSITLA SNSRSSSPAT ILQQNNKHFL INSNINIINT SLNNNNKNKD
EDNVNNNNGN NSNNNNNINN NNDEDNDEDN DENNNNNNNN NNNNNNNNNN NNNNTSNNDI
PDLSWTNYNS DSFNNNNNNN NIFNTFLSIS SPISDQQNTS PQIQEIISPL PSPPTVQQSD
VIQQTTTTSP SPLPSPQKQK QQQQRPLQKS SPPPTTITTS TTTTTQFNNN NLASSLGSTS
DLFNKSLISS FLNGQGISSI NNSNPFENSY KNQINSSTNS GTNTFSNTNT NINTTNSSNT
NVGYDQYSFN YSNGSNSNIN NSNNGLKSFL SNSNSSFSAL KNCSYDAKDM SSFTITLSNS
ELNTMIAESL NRSSASNSIV KSSNTVHTTS GGNNSLCSDG YNQNIISATT NTNTTTTNSS
STTTTTTATT TTASTTNNNI NNNNNNSTST TNSTKDITFL VCDNPDSTKE KSNVSNTSSI
ISASNLNRHI TPNSHMRPRG RSISESLIMS PINKESLNDI QRAIESEKIK KTKFEELKSI
LGEREYIIDI NDIQFIQKVG EGAFSEVWEG WWKGIHVAIK KLKIIGDEEQ FKERFIREVQ
NLKKGNHQNI VMFIGACYKP ACIITEYMAG GSLYNILHNP NSSTPKVKYS FPLVLKMATD
MALGLLHLHS ITIVHRDLTS QNILLDELGN IKISDFGLSR EKSREGSMTM TNGGICNPRW
RPPELTKNLG HYSEKVDVYC FSLVVWEILT GEIPFSDLDG SQASAQVAYA GLRPPIPEYC
DPELKLLLTQ CWEADPNDRP PFTYIVNKLK EISWNNPIGF VSDQFYQYSE PSTPRLALSN
QSSNSSSISL SPTKL
