ID   SPLE_STAA8              Reviewed;         238 AA.
AC   Q2FXC7; Q9KH47;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Serine protease SplE;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=splE; OrderedLocusNames=SAOUHSC_01936;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND INDUCTION.
RX   PubMed=11179322; DOI=10.1128/iai.69.3.1521-1527.2001;
RA   Reed S.B., Wesson C.A., Liou L.E., Trumble W.R., Schlievert P.M.,
RA   Bohach G.A., Bayles K.W.;
RT   "Molecular characterization of a novel Staphylococcus aureus serine
RT   protease operon.";
RL   Infect. Immun. 69:1521-1527(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Maximally expressed during early stationary phase.
CC       {ECO:0000269|PubMed:11179322}.
CC   -!- INDUCTION: Positively regulated by agr (accessory gene regulator).
CC       {ECO:0000269|PubMed:11179322}.
CC   -!- SIMILARITY: Belongs to the peptidase S1B family. {ECO:0000305}.
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DR   EMBL; AF271715; AAF97929.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD30999.1; -; Genomic_DNA.
DR   RefSeq; WP_001038759.1; NZ_LS483365.1.
DR   RefSeq; YP_500437.1; NC_007795.1.
DR   PDB; 5MM8; X-ray; 1.75 A; A=37-238.
DR   PDBsum; 5MM8; -.
DR   AlphaFoldDB; Q2FXC7; -.
DR   SMR; Q2FXC7; -.
DR   STRING; 1280.SAXN108_1842; -.
DR   MEROPS; S01.312; -.
DR   EnsemblBacteria; ABD30999; ABD30999; SAOUHSC_01936.
DR   GeneID; 3921020; -.
DR   KEGG; sao:SAOUHSC_01936; -.
DR   PATRIC; fig|93061.5.peg.1763; -.
DR   eggNOG; COG3591; Bacteria.
DR   HOGENOM; CLU_073589_2_0_9; -.
DR   OMA; MEIGEHI; -.
DR   PRO; PR:Q2FXC7; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR008256; Peptidase_S1B.
DR   InterPro; IPR008353; Peptidase_S1B_tx.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR028301; V8_his_AS.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR01774; EXFOLTOXIN.
DR   PRINTS; PR00839; V8PROTEASE.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS00672; V8_HIS; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Protease; Reference proteome; Secreted;
KW   Serine protease; Signal.
FT   SIGNAL          1..36
FT                   /evidence="ECO:0000255"
FT   CHAIN           37..238
FT                   /note="Serine protease SplE"
FT                   /id="PRO_0000359575"
FT   ACT_SITE        75
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        113
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        191
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   STRAND          39..42
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   HELIX           51..53
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          54..57
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          60..66
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          69..72
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   TURN            74..79
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          85..89
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          91..94
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          99..107
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          109..113
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          115..119
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          124..126
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   HELIX           131..134
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          149..154
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   TURN            158..162
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          166..176
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          179..182
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          194..196
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          202..208
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   STRAND          219..223
FT                   /evidence="ECO:0007829|PDB:5MM8"
FT   HELIX           226..234
FT                   /evidence="ECO:0007829|PDB:5MM8"
SQ   SEQUENCE   238 AA;  25679 MW;  66CA24C37613EBA4 CRC64;
     MNKNIIIKSI AALTILTSVT GVGTTVVEGI QQTAKAEHNV KLIKNTNVAP YNGVVSIGSG
     TGFIVGKNTI VTNKHVVAGM EIGAHIIAHP NGEYNNGGFY KVKKIVRYSG QEDIAILHVE
     DKAVHPKNRN FKDYTGILKI ASEAKENERI SIVGYPEPYI NKFQMYESTG KVLSVKGNMI
     ITDAFVEPGN SGSAVFNSKY EVVGVHFGGN GPGNKSTKGY GVYFSPEIKK FIADNTDK