SPL_ARATH
ID SPL_ARATH Reviewed; 314 AA.
AC O81836; A0MFA1; Q9S7B8;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Protein SPOROCYTELESS {ECO:0000303|PubMed:10465788};
DE AltName: Full=Protein NOZZLE {ECO:0000303|PubMed:10500234};
DE AltName: Full=Transcription factor SPL {ECO:0000303|Ref.6};
GN Name=SPL {ECO:0000303|PubMed:10465788};
GN Synonyms=NZZ {ECO:0000303|PubMed:10500234};
GN OrderedLocusNames=At4g27330 {ECO:0000312|Araport:AT4G27330};
GN ORFNames=F27G19.11 {ECO:0000312|EMBL:AL078467};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10465788; DOI=10.1101/gad.13.16.2108;
RA Yang W.C., Ye D., Xu J., Sundaresan V.;
RT "The SPOROCYTELESS gene of Arabidopsis is required for initiation of
RT sporogenesis and encodes a novel nuclear protein.";
RL Genes Dev. 13:2108-2117(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10500234; DOI=10.1073/pnas.96.20.11664;
RA Schiefthaler U., Balasubramanian S., Sieber P., Chevalier D., Wisman E.,
RA Schneitz K.;
RT "Molecular analysis of NOZZLE, a gene involved in pattern formation and
RT early sporogenesis during sex organ development in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:11664-11669(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION.
RX PubMed=10976054; DOI=10.1242/dev.127.19.4227;
RA Balasubramanian S., Schneitz K.;
RT "NOZZLE regulates proximal-distal pattern formation, cell proliferation and
RT early sporogenesis during ovule development in Arabidopsis thaliana.";
RL Development 127:4227-4238(2000).
RN [8]
RP FUNCTION.
RX PubMed=12183381; DOI=10.1242/dev.129.18.4291;
RA Balasubramanian S., Schneitz K.;
RT "NOZZLE links proximal-distal and adaxial-abaxial pattern formation during
RT ovule development in Arabidopsis thaliana.";
RL Development 129:4291-4300(2002).
RN [9]
RP INDUCTION BY AGAMOUS.
RX PubMed=15254538; DOI=10.1038/nature02733;
RA Ito T., Wellmer F., Yu H., Das P., Ito N., Alves-Ferreira M.,
RA Riechmann J.L., Meyerowitz E.M.;
RT "The homeotic protein AGAMOUS controls microsporogenesis by regulation of
RT SPOROCYTELESS.";
RL Nature 430:356-360(2004).
RN [10]
RP INTERACTION WITH YAB1; YAB3 AND YAB4.
RX PubMed=15299139; DOI=10.1104/pp.104.040154;
RA Sieber P., Petrascheck M., Barberis A., Schneitz K.;
RT "Organ polarity in Arabidopsis. NOZZLE physically interacts with members of
RT the YABBY family.";
RL Plant Physiol. 135:2172-2185(2004).
RN [11]
RP FUNCTION.
RX PubMed=18557819; DOI=10.1111/j.1469-8137.2008.02514.x;
RA Li L.C., Qin G.J., Tsuge T., Hou X.H., Ding M.Y., Aoyama T., Oka A.,
RA Chen Z., Gu H., Zhao Y., Qu L.J.;
RT "SPOROCYTELESS modulates YUCCA expression to regulate the development of
RT lateral organs in Arabidopsis.";
RL New Phytol. 179:751-764(2008).
RN [12]
RP FUNCTION.
RX PubMed=19726570; DOI=10.1104/pp.109.145896;
RA Liu X., Huang J., Parameswaran S., Ito T., Seubert B., Auer M.,
RA Rymaszewski A., Jia G., Owen H.A., Zhao D.;
RT "The SPOROCYTELESS/NOZZLE gene is involved in controlling stamen identity
RT in Arabidopsis.";
RL Plant Physiol. 151:1401-1411(2009).
RN [13]
RP INDUCTION BY ATXR3/SDG2.
RX PubMed=21037105; DOI=10.1105/tpc.110.079962;
RA Berr A., McCallum E.J., Menard R., Meyer D., Fuchs J., Dong A., Shen W.H.;
RT "Arabidopsis SET DOMAIN GROUP2 is required for H3K4 trimethylation and is
RT crucial for both sporophyte and gametophyte development.";
RL Plant Cell 22:3232-3248(2010).
RN [14]
RP INDUCTION BY TGA9 AND TGA10.
RC STRAIN=cv. Columbia;
RX PubMed=20805327; DOI=10.1104/pp.110.159111;
RA Murmu J., Bush M.J., Delong C., Li S., Xu M., Khan M., Malcolmson C.,
RA Fobert P.R., Zachgo S., Hepworth S.R.;
RT "Arabidopsis basic leucine-zipper transcription factors TGA9 and TGA10
RT interact with floral glutaredoxins ROXY1 and ROXY2 and are redundantly
RT required for anther development.";
RL Plant Physiol. 154:1492-1504(2010).
RN [15]
RP FUNCTION.
RX PubMed=22786869; DOI=10.1105/tpc.112.100164;
RA Bencivenga S., Simonini S., Benkova E., Colombo L.;
RT "The transcription factors BEL1 and SPL are required for cytokinin and
RT auxin signaling during ovule development in Arabidopsis.";
RL Plant Cell 24:2886-2897(2012).
RN [16]
RP FUNCTION, INTERACTION WITH TPL; TPR1; TPR2; TPR3; TPR4; TCP1; TCP2; TCP3;
RP TCP4; TCP5; TCP6; TCP8; TCP9; TCP10; TCP11; TCP15; TCP20; TCP21; TCP23;
RP TCP24; SPEAR1; SPEAR2; SPEAR3 AND SPEAR4, DOMAIN, AND SUBUNIT.
RX PubMed=25527103; DOI=10.1016/j.jgg.2014.08.009;
RA Chen G.H., Sun J.Y., Liu M., Liu J., Yang W.C.;
RT "SPOROCYTELESS is a novel embryophyte-specific transcription repressor that
RT interacts with TPL and TCP proteins in Arabidopsis.";
RL J. Genet. Genomics 41:617-625(2014).
RN [17]
RP FUNCTION, DOMAIN, MUTAGENESIS OF LEU-310; LEU-312 AND LEU-314, INTERACTION
RP WITH TPL; TPR1; TPR2; TPR3; TPR4; TCP2; TCP3; TCP4; TCP5; TCP10; TCP13;
RP TCP17 AND TCP24, AND DEVELOPMENTAL STAGE.
RX PubMed=25378179; DOI=10.1038/cr.2014.145;
RA Wei B., Zhang J., Pang C., Yu H., Guo D., Jiang H., Ding M., Chen Z.,
RA Tao Q., Gu H., Qu L.J., Qin G.;
RT "The molecular mechanism of sporocyteless/nozzle in controlling Arabidopsis
RT ovule development.";
RL Cell Res. 25:121-134(2015).
CC -!- FUNCTION: Transcriptional regulator of sporocyte development
CC (PubMed:10465788). Acts as an adapter-like transcriptional repressor
CC recruiting TPL/TPR corepressors to inhibit TCP transcription factors
CC (PubMed:25378179). Required for nucellus and embryo sac development
CC (PubMed:10500234). Plays a central role in patterning both the
CC proximal-distal and the adaxial-abaxial axes during ovule development
CC (PubMed:12183381). Involved in establishing the prospective chalaza of
CC the ovule and in controlling the cell number and the length of the
CC funiculus, and is required for the development of the integuments
CC (PubMed:10976054). Required, with BEL1, for cytokinin-induced PIN1
CC expression in ovules (PubMed:22786869). Involved in controlling stamen
CC identity (PubMed:19726570). May also regulate the morphology of lateral
CC organs by repressing auxin production (PubMed:18557819).
CC {ECO:0000269|PubMed:10465788, ECO:0000269|PubMed:10500234,
CC ECO:0000269|PubMed:10976054, ECO:0000269|PubMed:12183381,
CC ECO:0000269|PubMed:18557819, ECO:0000269|PubMed:19726570,
CC ECO:0000269|PubMed:22786869, ECO:0000269|PubMed:25378179}.
CC -!- SUBUNIT: Homodimer and heterodimer with SPEARs (PubMed:25527103).
CC Interacts in vitro with YAB1, YAB3 and YAB4 (PubMed:15299139).
CC Interacts (via EAR motif) with TPL, TPR1, TPR2, TPR3 and TPR4
CC (PubMed:25527103, PubMed:25378179). Interacts with SPEAR1, SPEAR2,
CC SPEAR3, SPEAR4, TCP1, TCP6, TCP8, TCP9, TCP11, TCP15, TCP20, TCP21 and
CC TCP23 (PubMed:25527103). Interacts with TCP2, TCP3, TCP4, TCP5, TCP10,
CC TCP13, TCP17 and TCP24 (PubMed:25527103, PubMed:25378179).
CC {ECO:0000269|PubMed:15299139, ECO:0000269|PubMed:25378179,
CC ECO:0000269|PubMed:25527103}.
CC -!- INTERACTION:
CC O81836; Q9S7W5: TCP13; NbExp=3; IntAct=EBI-1113588, EBI-4424877;
CC O81836; Q93Z00: TCP14; NbExp=3; IntAct=EBI-1113588, EBI-4424563;
CC O81836; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-1113588, EBI-4426144;
CC O81836; O22152: YAB1; NbExp=8; IntAct=EBI-1113588, EBI-1113627;
CC O81836; Q9XFB1: YAB3; NbExp=3; IntAct=EBI-1113588, EBI-1115657;
CC O81836; Q9LDT3: YAB4; NbExp=3; IntAct=EBI-1113588, EBI-1115523;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10465788}.
CC -!- TISSUE SPECIFICITY: Expressed in flower buds (PubMed:10465788,
CC PubMed:10500234). Not found in leaves, siliques and stems
CC (PubMed:10465788). Detected in rosette leaves, stem tissue and
CC seedlings (PubMed:10500234). {ECO:0000269|PubMed:10465788,
CC ECO:0000269|PubMed:10500234}.
CC -!- DEVELOPMENTAL STAGE: Expressed during microsporogenesis and
CC megasoprogenesis (PubMed:10465788). Expressed during ovule development
CC (PubMed:25378179). {ECO:0000269|PubMed:10465788,
CC ECO:0000269|PubMed:25378179}.
CC -!- INDUCTION: Up-regulated by the transcription factor AGAMOUS
CC (PubMed:15254538). Regulated by ATXR3/SDG2 via chromatin methylation
CC (PubMed:21037105). Triggered by TGA9 AND TGA10 in anthers
CC (PubMed:20805327). {ECO:0000269|PubMed:15254538,
CC ECO:0000269|PubMed:20805327, ECO:0000269|PubMed:21037105}.
CC -!- DOMAIN: Contains 1 EAR motif required for the interaction with TPL and
CC TPRs (PubMed:25378179). {ECO:0000269|PubMed:25378179}.
CC -!- DISRUPTION PHENOTYPE: Complete male and female sterility.
CC {ECO:0000269|PubMed:10465788}.
CC -!- SIMILARITY: Belongs to the NOZZLE family.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28652.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF146794; AAD37775.1; -; Genomic_DNA.
DR EMBL; AF159255; AAD45344.1; -; mRNA.
DR EMBL; AL030978; CAA19727.1; -; Genomic_DNA.
DR EMBL; AL078467; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL161566; CAB79588.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85325.1; -; Genomic_DNA.
DR EMBL; DQ446873; ABE66094.1; -; mRNA.
DR EMBL; DQ653227; ABK28652.1; ALT_SEQ; mRNA.
DR EMBL; AB493704; BAH30542.1; -; mRNA.
DR PIR; T05757; T05757.
DR RefSeq; NP_194463.1; NM_118867.2.
DR AlphaFoldDB; O81836; -.
DR IntAct; O81836; 18.
DR STRING; 3702.AT4G27330.1; -.
DR PaxDb; O81836; -.
DR PRIDE; O81836; -.
DR ProteomicsDB; 245198; -.
DR EnsemblPlants; AT4G27330.1; AT4G27330.1; AT4G27330.
DR GeneID; 828841; -.
DR Gramene; AT4G27330.1; AT4G27330.1; AT4G27330.
DR KEGG; ath:AT4G27330; -.
DR Araport; AT4G27330; -.
DR TAIR; locus:2131729; AT4G27330.
DR eggNOG; ENOG502R7KG; Eukaryota.
DR HOGENOM; CLU_881132_0_0_1; -.
DR InParanoid; O81836; -.
DR OMA; FGSYMEG; -.
DR OrthoDB; 1604526at2759; -.
DR PhylomeDB; O81836; -.
DR PRO; PR:O81836; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O81836; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:TAIR.
DR GO; GO:0048653; P:anther development; IMP:TAIR.
DR GO; GO:0048533; P:sporocyte differentiation; IMP:TAIR.
DR InterPro; IPR014855; NOZZLE.
DR InterPro; IPR040356; SPEAR.
DR PANTHER; PTHR33388; PTHR33388; 1.
DR PANTHER; PTHR33388:SF20; PTHR33388:SF20; 1.
DR Pfam; PF08744; NOZZLE; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..314
FT /note="Protein SPOROCYTELESS"
FT /id="PRO_0000435870"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 33..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 62..70
FT /note="SPL"
FT /evidence="ECO:0000305"
FT MOTIF 308..314
FT /note="EAR"
FT /evidence="ECO:0000305"
FT MUTAGEN 310
FT /note="L->A: Loss of interaction with TPL and loss of
FT repression activity; when associated with A-312 and A-314."
FT /evidence="ECO:0000269|PubMed:25378179"
FT MUTAGEN 312
FT /note="L->A: Loss of interaction with TPL and loss of
FT repression activity; when associated with A-310 and A-314."
FT /evidence="ECO:0000269|PubMed:25378179"
FT MUTAGEN 314
FT /note="L->A: Loss of interaction with TPL and loss of
FT repression activity; when associated with A-310 and A-312."
FT /evidence="ECO:0000269|PubMed:25378179"
FT CONFLICT 295
FT /note="K -> T (in Ref. 1; AAD45344 and 2; AAD37775)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 314 AA; 34078 MW; B0236D910544CEC3 CRC64;
MATSLFFMST DQNSVGNPND LLRNTRLVVN SSGEIRTETL KSRGRKPGSK TGQQKQKKPT
LRGMGVAKLE RQRIEEEKKQ LAAATVGDTS SVASISNNAT RLPVPVDPGV VLQGFPSSLG
SNRIYCGGVG SGQVMIDPVI SPWGFVETSS TTHELSSISN PQMFNASSNN RCDTCFKKKR
LDGDQNNVVR SNGGGFSKYT MIPPPMNGYD QYLLQSDHHQ RSQGFLYDHR IARAASVSAS
STTINPYFNE ATNHTGPMEE FGSYMEGNPR NGSGGVKEYE FFPGKYGERV SVVAKTSSLV
GDCSPNTIDL SLKL
