SPNE_AEDAE
ID SPNE_AEDAE Reviewed; 1374 AA.
AC Q16JS8;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Probable ATP-dependent RNA helicase spindle-E;
DE EC=3.6.4.13;
GN Name=spn-E; ORFNames=AAEL013235;
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
CC -!- FUNCTION: Probable ATP-binding RNA helicase which plays a central role
CC during gametogenesis by repressing transposable elements and preventing
CC their mobilization, which is essential for the germline integrity. Acts
CC via the piRNA metabolic process, which mediates the repression of
CC transposable elements during meiosis by forming complexes composed of
CC piRNAs and Piwi proteins and govern the methylation and subsequent
CC repression of transposons (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=Component of the
CC nuage, also named P granule, a germ-cell-specific organelle required to
CC repress transposon during meiosis. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000305}.
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DR EMBL; CH477994; EAT34533.1; -; Genomic_DNA.
DR RefSeq; XP_001663402.1; XM_001663352.1.
DR AlphaFoldDB; Q16JS8; -.
DR SMR; Q16JS8; -.
DR STRING; 7159.AAEL013235-PA; -.
DR GeneID; 5577484; -.
DR KEGG; aag:5577484; -.
DR VEuPathDB; VectorBase:AAEL023716; -.
DR eggNOG; KOG0920; Eukaryota.
DR HOGENOM; CLU_002601_1_0_1; -.
DR InParanoid; Q16JS8; -.
DR OMA; DPCRTVY; -.
DR PhylomeDB; Q16JS8; -.
DR Proteomes; UP000008820; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR Gene3D; 2.40.50.90; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR035437; SNase_OB-fold_sf.
DR InterPro; IPR002999; Tudor.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF00567; TUDOR; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Developmental protein; Differentiation; Helicase;
KW Hydrolase; Meiosis; Nucleotide-binding; Reference proteome;
KW RNA-mediated gene silencing; Spermatogenesis.
FT CHAIN 1..1374
FT /note="Probable ATP-dependent RNA helicase spindle-E"
FT /id="PRO_0000391910"
FT DOMAIN 46..212
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 265..447
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 866..931
FT /note="Tudor"
FT MOTIF 158..161
FT /note="DEAH box"
FT BINDING 59..66
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1374 AA; 157427 MW; 2188D1E3DD7BACE4 CRC64;
MSMADEDEEH TKALKAKEMM LPLFQKYNFT LKHNKLPIRH SKDDILARIR ENPVIVLEGP
TGCGKTTQVP QFILEEAYHR KEYCNIIVTQ PRKIAAMSIA KRVSEERKCE LGTLVGFKVG
LKECLSPDTR LLYCTTGVLL QSLINSKTMA NYTHVILDEI HEREVDMDFL LIVVRRFLAT
NSSKTKVILM SATIDSKAFA EYFKTPKKVG YLTAPIISVD RPRLYEVKEF YYDDLDKLRL
DFAIDYENPG ISSHMYTVAA KLVLVCDRLI ENMHGEERME YKPTVLIFLP GINEIDRMDH
VLRETLTRIV NPKEKPNLDI HRLHSILPAD EQVKVFRKPA PGQRKVILST NIAESSITVP
DVKFIIDFCL QRVLFTDTTT NFSTLRTEWA SQANCIQRQG RAGRVMDGRV YRLVDRRFYE
NQMRFSTSPE ILRCPLENVI LKAKLLEMGP PHSILALAMN PPDLSDIRNT VLQLKELGAL
VQTVKGNYEQ LDGDLTYMGR IMAKLPLDLR ISKLIILGYI FSVLEESIII AAGMNEKNIF
LNQNSVRGYS QKMYWADGSG SDGIAILNAY IAWKSRKEQA GNESDMASWT RRMSLDLKCL
MDMAELIREI KDRLNHVGMK EVSGPGRVVW SSREKTVILK VIMAGAFYPN YFIPMSVGGK
ELMERQSFTE LGGRDPCNTV FFTGFDHEKY IGPLYTVQIK KLLSEGDFSK HQNMKVMYDR
TTNRIFVTFL GSNDEHDQRG AFMPGKVHAD VYRAIKLRKL GSRNRIMEIR TMRQRDAIEF
ATEMNLGHWE DANGWVPRRK VIRNAHLSVI PAIHQASMVC QVTNVIHCNK FYLRPEDTKN
KDIFMDIHSK LNSRGYRLER FDPDWQFAVG QMVAAPLEEG SDRYARAVLK NYKNIRSTGD
VMWTVFFLDY GHSSVLGQSA FRKLDGPLAY MKEIPQRVFE ATLSEIQPSA IISPQGIWTA
QSINRFKEMT LGKIFVVDVY SVVNDVASVV LKKGDEVPIN SELIRLKFAQ YAEESYISKL
DHDMRERKQR EMSLDEDVRY EVYHSSKNNQ LKYEEDELED VSPPEEKLRC KVMLSGPHSP
LETTASSTVR SGVMKPVTIE NDSVNSILLD SNPQDTHEKM LVGAFVHEQG NRLVVRQASM
MPNIPGFGPL MALIFCPTCQ MKKDEEETRV VSILTGLGCD KNTGESLFPE HDLALTLDVV
LSDDDITEIN ALRYTMDTIL HTDQDQTVPK FGDVSIENLK AKVKQYIIKI LEHERKFMDI
RHAPNDYDWK LVEENNAAEA SSSSKKRKQD FNIYDNAIYP LLPPLGLLPV SAKQLDFLKR
HCRELHKLAL TDVNLPRHGI TCQMCNTILE TLPQLRIHLY SKLHRDRESQ IKFR
