SPNE_CULQU
ID SPNE_CULQU Reviewed; 1396 AA.
AC B0XDC4;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Probable ATP-dependent RNA helicase spindle-E;
DE EC=3.6.4.13;
GN Name=spn-E; ORFNames=CPIJ017541;
OS Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Culicini; Culex; Culex.
OX NCBI_TaxID=7176;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JHB;
RG The Broad Institute Genome Sequencing Platform;
RA Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.D.,
RA Hannick L.I., Megy K., O'Leary S.B., Pearson M., Haas B.J., Mauceli E.,
RA Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA Fraser-Liggett C.M., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT "Annotation of Culex pipiens quinquefasciatus.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable ATP-binding RNA helicase which plays a central role
CC during gametogenesis by repressing transposable elements and preventing
CC their mobilization, which is essential for the germline integrity. Acts
CC via the piRNA metabolic process, which mediates the repression of
CC transposable elements during meiosis by forming complexes composed of
CC piRNAs and Piwi proteins and govern the methylation and subsequent
CC repression of transposons (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=Component of the
CC nuage, also named P granule, a germ-cell-specific organelle required to
CC repress transposon during meiosis. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000305}.
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DR EMBL; DS232752; EDS45402.1; -; Genomic_DNA.
DR RefSeq; XP_001867646.1; XM_001867611.1.
DR AlphaFoldDB; B0XDC4; -.
DR SMR; B0XDC4; -.
DR STRING; 7176.CPIJ017541-PA; -.
DR EnsemblMetazoa; XM_038251917.1; XP_038107845.1; LOC6051164.
DR GeneID; 6051164; -.
DR KEGG; cqu:CpipJ_CPIJ017541; -.
DR VEuPathDB; VectorBase:CPIJ017541; -.
DR VEuPathDB; VectorBase:CQUJHB009375; -.
DR eggNOG; KOG0920; Eukaryota.
DR HOGENOM; CLU_002601_1_0_1; -.
DR InParanoid; B0XDC4; -.
DR OMA; DPCRTVY; -.
DR OrthoDB; 278674at2759; -.
DR PhylomeDB; B0XDC4; -.
DR Proteomes; UP000002320; Partially assembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR Gene3D; 2.40.50.90; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR035437; SNase_OB-fold_sf.
DR InterPro; IPR002999; Tudor.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF00567; TUDOR; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50304; TUDOR; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Developmental protein; Differentiation; Helicase;
KW Hydrolase; Meiosis; Nucleotide-binding; Reference proteome;
KW RNA-mediated gene silencing; Spermatogenesis.
FT CHAIN 1..1396
FT /note="Probable ATP-dependent RNA helicase spindle-E"
FT /id="PRO_0000391912"
FT DOMAIN 68..234
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 292..468
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 885..950
FT /note="Tudor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00211"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 180..183
FT /note="DEAH box"
FT BINDING 81..88
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1396 AA; 158391 MW; 2E06F4195481C69C CRC64;
MDEAAGPSTS RTSNLEDVDD EGASLAEEDE EHTKALKAKE MMAPLFQRYN FTMKKNDLPI
NWNKPEILDK IRSNAVVVLQ GATGCGKTTQ VPQYLLEEAF ERKEYCNIIV TQPRKIAAIS
IARRVAQERK CDLGTLVGYK VGLKEQHNED TRLLYVTTGV LLQSLINSKT MATYTHVILD
EVHEREVDMD FLLIVVRRLL STNSKKTKVI LMSATIDAKG FSEYFKIPKK SGYLSAPVIS
VERPRLHVVQ EFYFDDLEKL KVDFQIDYEA PGISEQMYNI AAKLVVVCDH LKGQEFGDSL
EYKPSIIIFL PGINEIEKME GALEKLIASI QNAAQRPNLL IMKLHSTLPA DDQTAVFRKP
GPNQRKVILS TNIAESSITV PDIKFVIDFC LQRILVTDTL TNFSTLRTEW ASKSNCIQRA
GRAGRLMSGR VYRLVDRRFF ENNMDVSTSP EILRCPLETV VLKAKLLEMG TPPSILALAM
APPNLDDIRN TILLLKEVGA MLRTVKGNYD QLDGDLTYLG RIMSKLPLDI RISKLIMLGY
IFSVMEEAVT IGAGMNVKNI FLNQNSVKTY SQKMYWADGS GSDAIAILNA YTAWKSRQEQ
AGDTADMYNW ARRMSLDRKS LIDMAELIHE VKDRLNRSGL KPVSGPNRVV WNAREKTVIL
KVILAGAFYP NYFIPMSVGG KELMERQSFT ELGGRDPCNT VFFTGFDHER YIGPLYTVQI
KKILSEGDYS KHQAMKVMYD RTTNRIFVTF LGTTDERDQR GSFMPGKVHA DVYRAIKLRK
LGARNRITEI RTMRQRDAID YATSAGLGHW EDANGWVPRR KIVRNAHLSV LPPIFREKVV
CQVTHVVHPN KFFLRPEDNR NKDIFREIHT QLNARQLHPF PADANFAMGQ MVAAPVQENQ
ETYARAVLRS YRNVRTTGSV SWTVFFIDYG HTAAMEETAF RQLDDSLGQL KDIPPRAFEA
TLTEIQPSAI ISPQGSWTTE SIHRFKELVL GKIFVAKVYS VVGVVASVEL SRDEIRMNSE
LIRLKYAQYA EESYISKLDH DHRERKQREI MMDENLRNEV YRSAELTQNT YEDDELEDVN
PPEDKLRCKV VLSGPHSPLE TSASATIRSS VMKPVSIESD SVNSILLDSN PQDTHEKLLV
AGGVNEQGNR LVLRQTSVMP NIPGFGAIMS LIFCPTAQLK KDKDETRVVT VLSGLGYDPS
TGEALYPEHD MALTLDVVLN DDDITNINAL RYTMDSILHT GEGQTDPKFG DASIQKLKLQ
VKQYIIKILE HERRFLDLRH APNDYNWRCD LNLTAGSSSS KKMKGDFNIY DKKAIFPLLE
PLNLLPVSAS QLTFLKKHCH ELHKLAHTDV QLPRHGITCQ LCNNVLETLP QLRIHLYSKL
HRDRESQIKY RSPQMY
