SPNS1_ARATH
ID SPNS1_ARATH Reviewed; 492 AA.
AC Q6NMN6; O49546;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Probable sphingolipid transporter spinster homolog 1;
GN OrderedLocusNames=At5g65687; ORFNames=F6H11.180;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
CC -!- FUNCTION: Probable sphingolipid transporter that plays a central role
CC in endosomes and/or lysosomes storage. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Lysosome membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Spinster (TC
CC 2.A.1.49) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10676.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA16689.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB010075; BAB10676.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL021684; CAA16689.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED98088.1; -; Genomic_DNA.
DR EMBL; BT011621; AAS47627.1; -; mRNA.
DR EMBL; BT014809; AAT41792.1; -; mRNA.
DR PIR; T05899; T05899.
DR RefSeq; NP_680469.1; NM_148164.5.
DR AlphaFoldDB; Q6NMN6; -.
DR SMR; Q6NMN6; -.
DR BioGRID; 21939; 1.
DR IntAct; Q6NMN6; 1.
DR STRING; 3702.AT5G65687.1; -.
DR TCDB; 2.A.1.49.5; the major facilitator superfamily (mfs).
DR iPTMnet; Q6NMN6; -.
DR PaxDb; Q6NMN6; -.
DR PRIDE; Q6NMN6; -.
DR ProteomicsDB; 228316; -.
DR EnsemblPlants; AT5G65687.1; AT5G65687.1; AT5G65687.
DR GeneID; 836697; -.
DR Gramene; AT5G65687.1; AT5G65687.1; AT5G65687.
DR KEGG; ath:AT5G65687; -.
DR Araport; AT5G65687; -.
DR TAIR; locus:504954889; AT5G65687.
DR eggNOG; KOG1330; Eukaryota.
DR HOGENOM; CLU_001265_55_3_1; -.
DR InParanoid; Q6NMN6; -.
DR OMA; IEYSAAW; -.
DR OrthoDB; 891881at2759; -.
DR PhylomeDB; Q6NMN6; -.
DR PRO; PR:Q6NMN6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6NMN6; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR CDD; cd17328; MFS_spinster_like; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR044770; MFS_spinster-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR23505; PTHR23505; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Endosome; Glycoprotein; Lipid transport; Lysosome; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..492
FT /note="Probable sphingolipid transporter spinster homolog
FT 1"
FT /id="PRO_0000415369"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 472..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 472
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862"
FT CARBOHYD 53
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 341
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 488
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 492 AA; 53509 MW; A55F06C28D70C0F3 CRC64;
MTRVGQRDSP AKEEAPPATK KRFLTPGRFV TILCIINLIN YVDRGVIASN GVNGSSKVCD
AKGVCSAGTG IQGEFNLTNF EDGLLSSAFM VGLLVASPIF AGLSKRFNPF KLIGVGLTVW
TIAVIGCGFS YNFWMIAVFR MFVGVGEASF ISLAAPYIDD SAPVARKNFW LGLFYMCIPA
GVALGYVFGG YIGNHLGWRW AFYIEAIAMA VFVILSFCIK PPQQLKGFAD KDSKKPSTSI
ETVAPTDAEA SQIKTKTPKS KNLVVLFGKD LKALFSEKVF IVNVLGYITY NFVIGAYSYW
GPKAGFGIYK MKNADMIFGG LTIICGIIGT LGGSYVLDRI NATLSNTFKL LAASTLLGAA
FCFTAFLMKN MYAFIALFAV GEILIFAPQA PVNFVCLHCV RPNLRPLSMA SSTVLIHILG
DVPSSPLYGK MQDHLKNWRK STLIITSILF LAAIIWGIGI FMNSVDRSNE VSEDDEVEED
KLESKTENST LA