SPNS2_ARATH
ID SPNS2_ARATH Reviewed; 484 AA.
AC Q9FLG8;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Probable sphingolipid transporter spinster homolog 2;
GN OrderedLocusNames=At5g64500; ORFNames=T12B11.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Probable sphingolipid transporter that plays a central role
CC in endosomes and/or lysosomes storage. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Lysosome membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Spinster (TC
CC 2.A.1.49) family. {ECO:0000305}.
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DR EMBL; AB010076; BAB11417.1; -; Genomic_DNA.
DR EMBL; AB025640; BAB11417.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED97908.1; -; Genomic_DNA.
DR EMBL; AY034956; AAK59462.1; -; mRNA.
DR EMBL; BT001973; AAN71972.1; -; mRNA.
DR RefSeq; NP_201255.1; NM_125846.3.
DR AlphaFoldDB; Q9FLG8; -.
DR SMR; Q9FLG8; -.
DR STRING; 3702.AT5G64500.1; -.
DR iPTMnet; Q9FLG8; -.
DR PaxDb; Q9FLG8; -.
DR PRIDE; Q9FLG8; -.
DR ProteomicsDB; 228371; -.
DR EnsemblPlants; AT5G64500.1; AT5G64500.1; AT5G64500.
DR GeneID; 836571; -.
DR Gramene; AT5G64500.1; AT5G64500.1; AT5G64500.
DR KEGG; ath:AT5G64500; -.
DR Araport; AT5G64500; -.
DR TAIR; locus:2179401; AT5G64500.
DR eggNOG; KOG1330; Eukaryota.
DR HOGENOM; CLU_001265_55_3_1; -.
DR InParanoid; Q9FLG8; -.
DR OMA; DQMVMSP; -.
DR OrthoDB; 891881at2759; -.
DR PhylomeDB; Q9FLG8; -.
DR PRO; PR:Q9FLG8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLG8; baseline and differential.
DR Genevisible; Q9FLG8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR CDD; cd17328; MFS_spinster_like; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR044770; MFS_spinster-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR23505; PTHR23505; 2.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Endosome; Glycoprotein; Lipid transport; Lysosome; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..484
FT /note="Probable sphingolipid transporter spinster homolog
FT 2"
FT /id="PRO_0000415370"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 436..456
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 466
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6NMN6"
FT CARBOHYD 62
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 484 AA; 52155 MW; 700D531B4E20B9C0 CRC64;
MDVDGEGDRG QNPRIMERDS DSIKDPISEP SWFTPKKLLF VFCVVNLINY IDRGAIASNG
INGSRGSCTS SGTCSSGSGI QGDFNLSNFE DGVLSSAFMV GLLVASPIFA SLAKSVNPFR
LIGVGLSIWT LAVIGCGLSF DFWSITICRM FVGVGEASFV SLAAPFIDDN APHDQKSAWL
AVFYMCIPTG YAFGYVYGGV VGSVLPWRAA FWGEAILMLP FAVLGFVIKP LHLKGFAPDD
TGKPRTDNLN VLPVGYGFSA VMKDLKLLLV DKVYVTNILG YIAYNFVLGA YSYWGPKAGY
NIYKMENADM IFGGVTVVCG IVGTLSGGVI LDYMDATISN AFKVLSVSTF IGAIFCFAAF
CFKSMYAFLA LFAVGELLVF ATQGPVNFIV LHCVKPSLRP LAMAMSTVSI HIFGDVPSSP
LVGVLQDYVN NWRVTSLVLT FVLFPAAAIW SIGIFLNSVD RYNEDSEPDA VTRESTAAPL
LQEA
