ID   SPNS2_XENTR             Reviewed;         513 AA.
AC   B0JZE1;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Sphingosine-1-phosphate transporter SPNS2 {ECO:0000305};
DE   AltName: Full=Protein spinster homolog 2 {ECO:0000305};
GN   Name=spns2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Lipid transporter that specifically mediates export of
CC       sphingosine-1-phosphate (sphing-4-enine 1-phosphate, S1P) and
CC       sphinganine-1-phosphate. {ECO:0000250|UniProtKB:A2SWM2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=sphing-4-enine 1-phosphate(in) = sphing-4-enine 1-
CC         phosphate(out); Xref=Rhea:RHEA:38667, ChEBI:CHEBI:60119;
CC         Evidence={ECO:0000250|UniProtKB:Q8IVW8};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=sphinganine 1-phosphate(in) = sphinganine 1-phosphate(out);
CC         Xref=Rhea:RHEA:38671, ChEBI:CHEBI:57939;
CC         Evidence={ECO:0000250|UniProtKB:Q8IVW8};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A2SWM2};
CC       Multi-pass membrane protein {ECO:0000255}. Endosome membrane
CC       {ECO:0000250|UniProtKB:A2SWM2}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Spinster (TC
CC       2.A.1.49) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC159142; AAI59143.1; -; mRNA.
DR   RefSeq; NP_001120149.1; NM_001126677.1.
DR   AlphaFoldDB; B0JZE1; -.
DR   SMR; B0JZE1; -.
DR   STRING; 8364.ENSXETP00000063338; -.
DR   GeneID; 100145187; -.
DR   KEGG; xtr:100145187; -.
DR   CTD; 124976; -.
DR   Xenbase; XB-GENE-5793583; spns2.
DR   eggNOG; KOG1330; Eukaryota.
DR   InParanoid; B0JZE1; -.
DR   OrthoDB; 891881at2759; -.
DR   Reactome; R-XTR-1660661; Sphingolipid de novo biosynthesis.
DR   Proteomes; UP000008143; Chromosome 2.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046624; F:sphingolipid transporter activity; ISS:UniProtKB.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006869; P:lipid transport; IBA:GO_Central.
DR   GO; GO:0002920; P:regulation of humoral immune response; ISS:UniProtKB.
DR   GO; GO:2000404; P:regulation of T cell migration; ISS:UniProtKB.
DR   GO; GO:0007605; P:sensory perception of sound; ISS:UniProtKB.
DR   GO; GO:0003376; P:sphingosine-1-phosphate receptor signaling pathway; ISS:UniProtKB.
DR   CDD; cd17328; MFS_spinster_like; 1.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR044770; MFS_spinster-like.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR23505; PTHR23505; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Endosome; Lipid transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..513
FT                   /note="Sphingosine-1-phosphate transporter SPNS2"
FT                   /id="PRO_0000363954"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        130..150
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        190..210
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        276..296
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        320..340
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        354..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        378..398
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        422..442
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        463..483
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   513 AA;  55447 MW;  908E691FF8AA396E CRC64;
     MCLESDGVGT VSNSQGCIPG AEERGLETLP GRMNPTSLDV KAVELESSSS KPDKAYNWKR
     ASVAAAGILS VGNVLNYLDR YTVAGVLLDI QQHFEVKDSG AGLLQTVFIC SFMVAAPIFG
     YLGDRFNRKV ILSSGIFFWS AITFSSSFIP KKYFWLLVLS RGLVGIGEAS YSTIAPTIIG
     DLFTKNTRTL MLSVFYFAIP LGSGLGYITG SSVKQVAGDW RWALRVSPVL GVITGTLLLI
     FVPTAKRGHA EQLKGSSWIR DMRGLIKNRS YVFSSLATST VSFATGALGM WIPLYLYRAQ
     VVQKSVEPCN IPPCSTKDSL IFGAITCLTG FLGVIIGAGA TKWCRRKTQR ADPLVCAVGM
     LGSAIFICLV FVAAKSSIIA AYICIFAGET LLFSNWAITA DMLMYVVIPT RRATAVALQS
     FTSHLLGDAG SPYLIGFISD LIQQSTTKSS LWEFLSLGYA LMLCPFVVVL GGMFFLATAL
     FFLEDREKAE KLEPCSDPFT VGNCSDSEEA SIL