SPNS3_MOUSE
ID SPNS3_MOUSE Reviewed; 514 AA.
AC Q9D232; B2RX04; Q3U0X5; Q8BYA9;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Protein spinster homolog 3;
GN Name=Spns3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Bone, Spleen, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Sphingolipid transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9D232-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9D232-2; Sequence=VSP_028199;
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Spinster (TC
CC 2.A.1.49) family. {ECO:0000305}.
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DR EMBL; AK020683; BAB32173.1; -; mRNA.
DR EMBL; AK041376; BAC30922.1; -; mRNA.
DR EMBL; AK156479; BAE33726.1; -; mRNA.
DR EMBL; AL662812; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466596; EDL12688.1; -; Genomic_DNA.
DR EMBL; BC150778; AAI50779.1; -; mRNA.
DR CCDS; CCDS24987.1; -. [Q9D232-1]
DR RefSeq; NP_084208.1; NM_029932.3. [Q9D232-1]
DR AlphaFoldDB; Q9D232; -.
DR SMR; Q9D232; -.
DR STRING; 10090.ENSMUSP00000090617; -.
DR iPTMnet; Q9D232; -.
DR PhosphoSitePlus; Q9D232; -.
DR MaxQB; Q9D232; -.
DR PaxDb; Q9D232; -.
DR PRIDE; Q9D232; -.
DR Antibodypedia; 11205; 63 antibodies from 13 providers.
DR DNASU; 77577; -.
DR Ensembl; ENSMUST00000021154; ENSMUSP00000021154; ENSMUSG00000020798. [Q9D232-2]
DR Ensembl; ENSMUST00000092940; ENSMUSP00000090617; ENSMUSG00000020798. [Q9D232-1]
DR GeneID; 77577; -.
DR KEGG; mmu:77577; -.
DR UCSC; uc007jzb.1; mouse. [Q9D232-1]
DR UCSC; uc011xyk.1; mouse. [Q9D232-2]
DR CTD; 201305; -.
DR MGI; MGI:1924827; Spns3.
DR VEuPathDB; HostDB:ENSMUSG00000020798; -.
DR eggNOG; KOG1330; Eukaryota.
DR GeneTree; ENSGT00390000005976; -.
DR HOGENOM; CLU_001265_5_12_1; -.
DR InParanoid; Q9D232; -.
DR OMA; WHWAFRI; -.
DR OrthoDB; 891881at2759; -.
DR PhylomeDB; Q9D232; -.
DR TreeFam; TF314395; -.
DR BioGRID-ORCS; 77577; 7 hits in 71 CRISPR screens.
DR PRO; PR:Q9D232; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9D232; protein.
DR Bgee; ENSMUSG00000020798; Expressed in granulocyte and 48 other tissues.
DR Genevisible; Q9D232; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR CDD; cd17328; MFS_spinster_like; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR044770; MFS_spinster-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR23505; PTHR23505; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Lipid transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..514
FT /note="Protein spinster homolog 3"
FT /id="PRO_0000305047"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 482..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 493..514
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 71..92
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028199"
FT CONFLICT 17
FT /note="S -> G (in Ref. 3; EDL12688 and 4; AAI50779)"
FT /evidence="ECO:0000305"
FT CONFLICT 40
FT /note="P -> S (in Ref. 3; EDL12688 and 4; AAI50779)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="I -> V (in Ref. 3; EDL12688 and 4; AAI50779)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="K -> E (in Ref. 1; BAE33726)"
FT /evidence="ECO:0000305"
FT CONFLICT 458
FT /note="A -> T (in Ref. 1; BAE33726)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 514 AA; 55456 MW; DCCBACFEC340CE74 CRC64;
MSTECLKPQT GGPQSQSLSQ GGQYGALASG TCLPPSTPVP WSLPRWRAYL AAAVLCYINL
LNYMNWFIIP GVLLDVQKYF HISDSHAGLL QTVFISCLLV SAPVFGYLGD RYNRKAILSF
GILLWSGAGL SSSFISYQYS WLFFLSRGFV GTGAASYSTI APTVLGDLFV KDQRTCALAV
FYIFIPVGSG LGYVLGSTVA ELTGNWRWAL RLMPCLDAMA LALLILLVPD VPRGAAEKQG
EVAVRAPRSS WCEDVRYLGR NWSFVFSTLG VTAIAFVTGA LGFWAPKFLF EARVVHGLQL
PCFQEQCHSQ DSLIFGALTV ATGIIGVMLG AEASRRYKKV NPRAEPLICA SSLFATAPCL
YLALILASRT LLASYVFLAL GELLLSCNWA VVADILLSVV VPRCRGTAEA LQITVAHVLG
DAGSPYLTGL ISSVLQAERP DSYLQHFLSL QHSFLCCAFA IVLGGGFFLL TALHLEKDQA
RARQPGKGTL DSKDIASRNT ESQGLLSGTS TPTE
