SPO11_DROME
ID SPO11_DROME Reviewed; 331 AA.
AC Q7KPA5;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Meiotic recombination protein W68 {ECO:0000303|PubMed:9744869};
DE AltName: Full=SPO11 protein homolog {ECO:0000303|PubMed:9744869};
GN Name=mei-W68 {ECO:0000303|PubMed:9744869}; ORFNames=CG7753;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=9744869; DOI=10.1101/gad.12.18.2932;
RA McKim K.S., Hayashi-Hagihara A.;
RT "mei-W68 in Drosophila melanogaster encodes a Spo11 homolog: evidence that
RT the mechanism for initiating meiotic recombination is conserved.";
RL Genes Dev. 12:2932-2942(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.E.;
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for meiotic recombination (PubMed:9744869). Together
CC with mei-P22, mediates DNA cleavage that forms the double-strand breaks
CC (DSB) that initiate meiotic recombination (By similarity).
CC {ECO:0000250|UniProtKB:Q9WTK8, ECO:0000269|PubMed:9744869}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q57815};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TOP6A family. {ECO:0000305}.
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DR EMBL; AF085276; AAC61735.1; -; mRNA.
DR EMBL; AE013599; AAF57553.1; -; Genomic_DNA.
DR EMBL; BT120379; ADD16459.1; -; mRNA.
DR RefSeq; NP_995901.1; NM_206179.3.
DR AlphaFoldDB; Q7KPA5; -.
DR SMR; Q7KPA5; -.
DR STRING; 7227.FBpp0085657; -.
DR PaxDb; Q7KPA5; -.
DR DNASU; 2768853; -.
DR EnsemblMetazoa; FBtr0086461; FBpp0085657; FBgn0002716.
DR GeneID; 2768853; -.
DR KEGG; dme:Dmel_CG7753; -.
DR UCSC; CG7753-RA; d. melanogaster.
DR CTD; 2768853; -.
DR FlyBase; FBgn0002716; mei-W68.
DR VEuPathDB; VectorBase:FBgn0002716; -.
DR eggNOG; KOG2795; Eukaryota.
DR GeneTree; ENSGT00390000001787; -.
DR HOGENOM; CLU_037229_1_1_1; -.
DR InParanoid; Q7KPA5; -.
DR OMA; NWGEARF; -.
DR OrthoDB; 1272299at2759; -.
DR PhylomeDB; Q7KPA5; -.
DR BioGRID-ORCS; 2768853; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 2768853; -.
DR PRO; PR:Q7KPA5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0002716; Expressed in saliva-secreting gland and 8 other tissues.
DR ExpressionAtlas; Q7KPA5; baseline and differential.
DR GO; GO:0000228; C:nuclear chromosome; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR GO; GO:0042139; P:early meiotic recombination nodule assembly; IMP:FlyBase.
DR GO; GO:0042140; P:late meiotic recombination nodule assembly; IMP:FlyBase.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IBA:GO_Central.
DR GO; GO:0000706; P:meiotic DNA double-strand break processing; IDA:FlyBase.
DR GO; GO:0030717; P:oocyte karyosome formation; IGI:FlyBase.
DR GO; GO:0048477; P:oogenesis; IGI:FlyBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:FlyBase.
DR CDD; cd00223; TOPRIM_TopoIIB_SPO; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR002815; Spo11/TopoVI_A.
DR InterPro; IPR013049; Spo11/TopoVI_A_N.
DR InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR InterPro; IPR004085; TopoVI_A.
DR InterPro; IPR034136; TOPRIM_Topo6A/Spo11.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR10848; PTHR10848; 1.
DR Pfam; PF04406; TP6A_N; 1.
DR PRINTS; PR01550; TOP6AFAMILY.
DR PRINTS; PR01552; TPISMRASE6A.
DR SUPFAM; SSF56726; SSF56726; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Hydrolase; Isomerase; Magnesium; Meiosis; Metal-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..331
FT /note="Meiotic recombination protein W68"
FT /id="PRO_0000436507"
FT ACT_SITE 81
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q9M4A2"
FT BINDING 167
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q57815"
FT BINDING 221
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q57815"
SQ SEQUENCE 331 AA; 37064 MW; 456CE0A49383EA8A CRC64;
MDEFSENIER IALELLSNLV HGNATLSVPR NSSGNVISEY RRVSYNNRGS RHSFCVLIYM
LSRVHRLQVR GGSFTVRGLY YDNPLLVRSQ SRIAEARLDV CRMLRTSPLS LGILAASKGL
VAGDLRLLMT NGDVLDSSLY GGPLTLPTDP EKIDRIETLA EFVLIVEKES VFESLLSRNV
FGTFERRFIL ITGKGYPDCC TRRIVHRLTE ENQLAAYILV DADPFGVEIM LVYRHGSKSM
SFSSQGLTTP ALRWIGLHPS EIPALGTGAV ALVAGDNKKI NDLLARHDLE PGVRQELRML
QDVQLKAEIE SVIDFLTDDY IPNKINRNLF L
