ID   SPO11_ENCCU             Reviewed;         303 AA.
AC   Q8SVS9;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Probable meiosis-specific protein SPO11 homolog;
GN   Name=SPO11; OrderedLocusNames=ECU04_1110;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
CC   -!- FUNCTION: Required for meiotic recombination. Mediates DNA cleavage
CC       that forms the double-strand breaks (DSB) that initiate meiotic
CC       recombination (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q57815};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TOP6A family. {ECO:0000305}.
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DR   EMBL; AL590444; CAD25299.1; -; Genomic_DNA.
DR   RefSeq; NP_584795.1; NM_001041145.1.
DR   AlphaFoldDB; Q8SVS9; -.
DR   SMR; Q8SVS9; -.
DR   STRING; 284813.Q8SVS9; -.
DR   GeneID; 858943; -.
DR   KEGG; ecu:ECU04_1110; -.
DR   VEuPathDB; MicrosporidiaDB:ECU04_1110; -.
DR   HOGENOM; CLU_037229_4_0_1; -.
DR   InParanoid; Q8SVS9; -.
DR   OMA; MEIEIFT; -.
DR   OrthoDB; 1272299at2759; -.
DR   Proteomes; UP000000819; Chromosome IV.
DR   GO; GO:0005694; C:chromosome; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006259; P:DNA metabolic process; IEA:InterPro.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   CDD; cd00223; TOPRIM_TopoIIB_SPO; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR002815; Spo11/TopoVI_A.
DR   InterPro; IPR013049; Spo11/TopoVI_A_N.
DR   InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR   InterPro; IPR034136; TOPRIM_Topo6A/Spo11.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR10848; PTHR10848; 1.
DR   Pfam; PF04406; TP6A_N; 1.
DR   PRINTS; PR01550; TOP6AFAMILY.
DR   SUPFAM; SSF56726; SSF56726; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Hydrolase; Magnesium; Meiosis; Metal-binding; Nucleus;
KW   Reference proteome; Sporulation.
FT   CHAIN           1..303
FT                   /note="Probable meiosis-specific protein SPO11 homolog"
FT                   /id="PRO_0000388416"
FT   ACT_SITE        57
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M4A2"
FT   BINDING         144
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q57815"
FT   BINDING         196
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q57815"
SQ   SEQUENCE   303 AA;  34749 MW;  BFB8D57252E1FAC2 CRC64;
     MALAPTSSAI SGSLRSSMLK LLMRLKSRTL ATRLRLYEII IEMQELGITR NEREIFYMDV
     NVFRTQSVVR RLVSSIASEL QISKHDLGVR NTLKGIFIGR LGFVRHHGLG MVEMSSKGGC
     PQLIPDMSDI AEVLCDYKKT VVVEKDTVLQ RIASEIEREK CLEEILFVCG KGYPCKNTVL
     LLKMIEHKTA VAGLFDLDPF GIHIFCIYKY GSKETPDIRV ETIMRIGVCM EDVLEKNAYK
     DVFVKLNVHD LKMINRLVRF GELSADLLFL RKIDGKVEME ALFSKEPRRL RYFLFRMLER
     ISS