ID   SPO11_NOSCE             Reviewed;         271 AA.
AC   C4VB43;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Probable meiosis-specific protein SPO11 homolog;
GN   Name=SPO11; ORFNames=NCER_101961;
OS   Nosema ceranae (strain BRL01) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Nosematidae; Nosema.
OX   NCBI_TaxID=578460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BRL01;
RX   PubMed=19503607; DOI=10.1371/journal.ppat.1000466;
RA   Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B.,
RA   Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.;
RT   "Genomic analyses of the microsporidian Nosema ceranae, an emergent
RT   pathogen of honey bees.";
RL   PLoS Pathog. 5:E1000466-E1000466(2009).
CC   -!- FUNCTION: Required for meiotic recombination. Mediates DNA cleavage
CC       that forms the double-strand breaks (DSB) that initiate meiotic
CC       recombination (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q57815};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TOP6A family. {ECO:0000305}.
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DR   EMBL; ACOL01000381; EEQ81557.1; -; Genomic_DNA.
DR   RefSeq; XP_002995228.1; XM_002995182.1.
DR   AlphaFoldDB; C4VB43; -.
DR   SMR; C4VB43; -.
DR   STRING; 578460.C4VB43; -.
DR   PRIDE; C4VB43; -.
DR   EnsemblFungi; EEQ81557; EEQ81557; NCER_101961.
DR   KEGG; nce:NCER_101961; -.
DR   VEuPathDB; MicrosporidiaDB:NCER_101961; -.
DR   HOGENOM; CLU_037229_4_0_1; -.
DR   InParanoid; C4VB43; -.
DR   OMA; PCERTRE; -.
DR   Proteomes; UP000009082; Unassembled WGS sequence.
DR   GO; GO:0005694; C:chromosome; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006259; P:DNA metabolic process; IEA:InterPro.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR002815; Spo11/TopoVI_A.
DR   InterPro; IPR013049; Spo11/TopoVI_A_N.
DR   InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR10848; PTHR10848; 1.
DR   Pfam; PF04406; TP6A_N; 1.
DR   PRINTS; PR01550; TOP6AFAMILY.
DR   SUPFAM; SSF56726; SSF56726; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Hydrolase; Magnesium; Meiosis; Metal-binding; Nucleus;
KW   Reference proteome; Sporulation.
FT   CHAIN           1..271
FT                   /note="Probable meiosis-specific protein SPO11 homolog"
FT                   /id="PRO_0000388417"
FT   ACT_SITE        53
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M4A2"
FT   BINDING         136
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q57815"
FT   BINDING         182
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q57815"
SQ   SEQUENCE   271 AA;  32095 MW;  391C151430B239F6 CRC64;
     MKNNLKVKLE ELVIYILKNI KRYPNIIQRL KFYEQIHLGL TLNKIKNKRE IYYNSVSIFK
     KQSIVDMLIK DTCNKLKCTQ SDLLISTTLK GVFYGNITFY KNNKLVHVNL KGTSLIPDMN
     QIDRIEYTQK KCLIIEKDTI FSKTVREYIS NDILFICGKG YPCRNTLLLV NKLNIRKYGI
     FDFDPYGLEI CTKYPSIKKI GIDIKDINLI DKQHFMILNK YDIRKINTLL KQKVYEIELF
     YMLKNNIKIE IEGLFSAKGF DINNYFYTKI I