SPO11_ORYSI
ID SPO11_ORYSI Reviewed; 381 AA.
AC Q5ZPV8;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Meiotic recombination protein SPO11-1;
DE Short=OsSPO11-1;
DE AltName: Full=Topoisomerase VI subunit A1;
DE Short=OsTOP6A1;
GN Name=SPO11-1; ORFNames=OsI_13532;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Pusa Basmati;
RX PubMed=17116242; DOI=10.1111/j.1742-4658.2006.05518.x;
RA Jain M., Tyagi A.K., Khurana J.P.;
RT "Overexpression of putative topoisomerase 6 genes from rice confers stress
RT tolerance in transgenic Arabidopsis plants.";
RL FEBS J. 273:5245-5260(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=18071708; DOI=10.1007/s00299-007-0491-8;
RA Jain M., Tyagi A.K., Khurana J.P.;
RT "Constitutive expression of a meiotic recombination protein gene homolog,
RT OsTOP6A1, from rice confers abiotic stress tolerance in transgenic
RT Arabidopsis plants.";
RL Plant Cell Rep. 27:767-778(2008).
CC -!- FUNCTION: Required for meiotic recombination. Mediates DNA cleavage
CC that forms the double-strand breaks (DSB) that initiate meiotic
CC recombination (By similarity). May be involved in plant growth and
CC development, and stress tolerance. {ECO:0000250,
CC ECO:0000269|PubMed:18071708}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250, ECO:0000250|UniProtKB:Q57815};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18071708}.
CC -!- TISSUE SPECIFICITY: Highly expressed in flowers before pollination.
CC Expressed in roots and shoots. {ECO:0000269|PubMed:17116242}.
CC -!- SIMILARITY: Belongs to the TOP6A family. {ECO:0000305}.
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DR EMBL; AJ549926; CAD71263.1; -; mRNA.
DR EMBL; CM000128; EEC76191.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5ZPV8; -.
DR SMR; Q5ZPV8; -.
DR STRING; 39946.Q5ZPV8; -.
DR EnsemblPlants; BGIOSGA013572-TA; BGIOSGA013572-PA; BGIOSGA013572.
DR Gramene; BGIOSGA013572-TA; BGIOSGA013572-PA; BGIOSGA013572.
DR HOGENOM; CLU_037229_1_1_1; -.
DR OMA; TKRNIYY; -.
DR Proteomes; UP000007015; Chromosome 3.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:EnsemblPlants.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051026; P:chiasma assembly; IEA:EnsemblPlants.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:EnsemblPlants.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IEA:EnsemblPlants.
DR CDD; cd00223; TOPRIM_TopoIIB_SPO; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR013048; Meiotic_Spo11.
DR InterPro; IPR002815; Spo11/TopoVI_A.
DR InterPro; IPR013049; Spo11/TopoVI_A_N.
DR InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR InterPro; IPR034136; TOPRIM_Topo6A/Spo11.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR10848; PTHR10848; 1.
DR Pfam; PF04406; TP6A_N; 1.
DR PRINTS; PR01551; SPO11HOMOLOG.
DR PRINTS; PR01550; TOP6AFAMILY.
DR SUPFAM; SSF56726; SSF56726; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Metal-binding; Nucleus; Reference proteome.
FT CHAIN 1..381
FT /note="Meiotic recombination protein SPO11-1"
FT /id="PRO_0000429775"
FT ACT_SITE 123
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q9M4A2"
FT BINDING 209
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q57815"
FT BINDING 261
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q57815"
SQ SEQUENCE 381 AA; 43560 MW; 98534E98C9AE6680 CRC64;
MAGREKRRRV AALDGEERRR RQEEAATLLH RIRGLVRWVV AEVAAGRSPT VALHRYQNYC
SSASAAAASP CACSYDVPVG TDVLSLLHRG SHASRLNVLL RVLLVVQQLL QQNKHCSKRD
IYYMYPSIFQ EQAVVDRAIN DICVLFKCSR HNLNVVPVAK GLVMGWIRFL EGEKEVYCVT
NVNAAFSIPV SIEAIKDVVS VADYILIVEK ETVFQRLAND KFCERNRCIV ITGRGYPDIP
TRRFLRYLVE QLHLPVYCLV DADPYGFDIL ATYKFGSLQL AYDANFLRVP DIRWLGVFTS
DFEDYRLPDC CLLHLSSEDR RKAEGILSRC YLHREAPQWR LELEAMLQKG VKFEIEALSA
CSISFLSEEY IPKKIKQGRH I
