ID   SPO11_ORYSJ             Reviewed;         381 AA.
AC   Q7Y021; A0A0P0W3J0;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Meiotic recombination protein SPO11-1;
DE            Short=OsSPO11-1;
DE   AltName: Full=OsSPO11A;
DE   AltName: Full=Putative topoisomerase VI subunit A1;
DE            Short=OsTOP6A1;
GN   Name=SPO11-1; OrderedLocusNames=Os03g0752200, LOC_Os03g54084;
GN   ORFNames=OJ1112_G08.6, OsJ_12593, OSJNBa0047E24.8;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=21637817; DOI=10.1371/journal.pone.0020327;
RA   An X.J., Deng Z.Y., Wang T.;
RT   "OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates
RT   double-strand DNA cleavage and interacts with OsTopVIB.";
RL   PLoS ONE 6:E20327-E20327(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare;
RA   Yamada K., Kawagishi-Kobayashi M., Shingu Y., Mikawa T., Wakasa K.,
RA   Shibata T.;
RT   "Isolation and characterization of rice SPO11 genes.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [6]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [8]
RP   FUNCTION.
RC   STRAIN=cv. Yandao;
RX   PubMed=20625906; DOI=10.1007/s00412-010-0284-7;
RA   Yu H., Wang M., Tang D., Wang K., Chen F., Gong Z., Gu M., Cheng Z.;
RT   "OsSPO11-1 is essential for both homologous chromosome pairing and
RT   crossover formation in rice.";
RL   Chromosoma 119:625-636(2010).
CC   -!- FUNCTION: Required for meiotic recombination. Mediates DNA cleavage
CC       that forms the double-strand breaks (DSB) that initiate meiotic
CC       recombination. Is essential for both homologous chromosomes pairing and
CC       crossover formation during meiosis. {ECO:0000269|PubMed:20625906}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q57815};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- MISCELLANEOUS: Plants silencing TPO6A1 have no apparent growth defects
CC       during vegetative development, but most of the plant are completely
CC       sterile due to empty and shrunken mature pollen grains, and defect in
CC       the macrospores. {ECO:0000305|PubMed:20625906}.
CC   -!- SIMILARITY: Belongs to the TOP6A family. {ECO:0000305}.
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DR   EMBL; GU170363; ACZ52153.1; -; mRNA.
DR   EMBL; AB219537; BAF65344.1; -; mRNA.
DR   EMBL; AC092556; AAR87259.1; -; Genomic_DNA.
DR   EMBL; AC135225; AAP68363.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98916.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAH92371.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS86422.1; -; Genomic_DNA.
DR   EMBL; CM000140; EEE59941.1; -; Genomic_DNA.
DR   RefSeq; XP_015630524.1; XM_015775038.1.
DR   AlphaFoldDB; Q7Y021; -.
DR   SMR; Q7Y021; -.
DR   STRING; 4530.OS03T0752200-01; -.
DR   PaxDb; Q7Y021; -.
DR   PRIDE; Q7Y021; -.
DR   EnsemblPlants; Os03t0752200-01; Os03t0752200-01; Os03g0752200.
DR   GeneID; 9267055; -.
DR   Gramene; Os03t0752200-01; Os03t0752200-01; Os03g0752200.
DR   KEGG; osa:9267055; -.
DR   eggNOG; KOG2795; Eukaryota.
DR   HOGENOM; CLU_037229_1_1_1; -.
DR   OMA; TKRNIYY; -.
DR   OrthoDB; 1272299at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q7Y021; OS.
DR   GO; GO:0000228; C:nuclear chromosome; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051026; P:chiasma assembly; IEA:EnsemblPlants.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IMP:UniProtKB.
DR   GO; GO:0042138; P:meiotic DNA double-strand break formation; IBA:GO_Central.
DR   GO; GO:0000706; P:meiotic DNA double-strand break processing; IBA:GO_Central.
DR   GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR   CDD; cd00223; TOPRIM_TopoIIB_SPO; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR013048; Meiotic_Spo11.
DR   InterPro; IPR002815; Spo11/TopoVI_A.
DR   InterPro; IPR013049; Spo11/TopoVI_A_N.
DR   InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR   InterPro; IPR034136; TOPRIM_Topo6A/Spo11.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR10848; PTHR10848; 1.
DR   Pfam; PF04406; TP6A_N; 1.
DR   PRINTS; PR01551; SPO11HOMOLOG.
DR   PRINTS; PR01550; TOP6AFAMILY.
DR   SUPFAM; SSF56726; SSF56726; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Magnesium; Metal-binding; Nucleus; Reference proteome.
FT   CHAIN           1..381
FT                   /note="Meiotic recombination protein SPO11-1"
FT                   /id="PRO_0000429776"
FT   ACT_SITE        123
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M4A2"
FT   BINDING         209
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q57815"
FT   BINDING         261
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q57815"
SQ   SEQUENCE   381 AA;  43560 MW;  98534E98C9AE6680 CRC64;
     MAGREKRRRV AALDGEERRR RQEEAATLLH RIRGLVRWVV AEVAAGRSPT VALHRYQNYC
     SSASAAAASP CACSYDVPVG TDVLSLLHRG SHASRLNVLL RVLLVVQQLL QQNKHCSKRD
     IYYMYPSIFQ EQAVVDRAIN DICVLFKCSR HNLNVVPVAK GLVMGWIRFL EGEKEVYCVT
     NVNAAFSIPV SIEAIKDVVS VADYILIVEK ETVFQRLAND KFCERNRCIV ITGRGYPDIP
     TRRFLRYLVE QLHLPVYCLV DADPYGFDIL ATYKFGSLQL AYDANFLRVP DIRWLGVFTS
     DFEDYRLPDC CLLHLSSEDR RKAEGILSRC YLHREAPQWR LELEAMLQKG VKFEIEALSA
     CSISFLSEEY IPKKIKQGRH I