ID   SPO12_ORYSI             Reviewed;         385 AA.
AC   Q5ZPI9;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Meiotic recombination protein SPO11-2;
DE            Short=OsSPO11-2;
DE   AltName: Full=Topoisomerase 6 subunit A2;
DE            Short=OsTOP6A2;
GN   Name=SPO11-2;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TOP6B, TISSUE SPECIFICITY, AND
RP   INDUCTION.
RC   STRAIN=cv. Pusa Basmati;
RX   PubMed=17116242; DOI=10.1111/j.1742-4658.2006.05518.x;
RA   Jain M., Tyagi A.K., Khurana J.P.;
RT   "Overexpression of putative topoisomerase 6 genes from rice confers stress
RT   tolerance in transgenic Arabidopsis plants.";
RL   FEBS J. 273:5245-5260(2006).
CC   -!- FUNCTION: Required for meiotic recombination. Mediates DNA cleavage
CC       that forms the double-strand breaks (DSB) that initiate meiotic
CC       recombination (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q57815};
CC   -!- SUBUNIT: Interacts with TOP6B. {ECO:0000269|PubMed:17116242}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in flowers before pollination.
CC       Expressed in roots and shoots. {ECO:0000269|PubMed:17116242}.
CC   -!- INDUCTION: By auxin and benzylaminopurine.
CC       {ECO:0000269|PubMed:17116242}.
CC   -!- SIMILARITY: Belongs to the TOP6A family. {ECO:0000305}.
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DR   EMBL; AJ605583; CAE53723.1; -; mRNA.
DR   AlphaFoldDB; Q5ZPI9; -.
DR   SMR; Q5ZPI9; -.
DR   GO; GO:0005694; C:chromosome; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006259; P:DNA metabolic process; IEA:InterPro.
DR   CDD; cd00223; TOPRIM_TopoIIB_SPO; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR002815; Spo11/TopoVI_A.
DR   InterPro; IPR013049; Spo11/TopoVI_A_N.
DR   InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR   InterPro; IPR034136; TOPRIM_Topo6A/Spo11.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR10848; PTHR10848; 1.
DR   Pfam; PF04406; TP6A_N; 1.
DR   PRINTS; PR01550; TOP6AFAMILY.
DR   SUPFAM; SSF56726; SSF56726; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Metal-binding; Nucleus.
FT   CHAIN           1..385
FT                   /note="Meiotic recombination protein SPO11-2"
FT                   /id="PRO_0000429777"
FT   ACT_SITE        126
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M4A2"
FT   BINDING         219
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q57815"
FT   BINDING         272
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q57815"
SQ   SEQUENCE   385 AA;  42755 MW;  2D0E61ED09836A1E CRC64;
     MAEAGVAAAS LFGADRRLCS ADILPPAEVR ARIEVAVLNF LAALTDPAAP AISALPLISR
     GAANRGLRRA LLRDDVSSVY LSYASCKRSL TRANDAKAFV RVWKVMEMCY KILGEGKLVT
     LRELFYTLLS ESPTYFTCQR HVNQTVQDVV SLLRCTRQSL GIMASSRGAL IGRLVLQGPE
     EEHVDCSILG PSGHAITGDL NVLSKLIFSS DARYIIVVEK DAIFQRLAED RIYSHLPCIL
     ITAKGYPDLA TRFILHRLSQ TYPNMPIFAL VDWNPAGLAI LCTYKYGSIS MGLESYRYAC
     NVKWLGLRGD DLQLIPQSAY QELKPRDLQI AKSLLSSKFL QDKHRAELTL MLETGKRAEI
     EALYSHGFDF LGKYVARKIV QGDYI