SPO12_ORYSJ
ID SPO12_ORYSJ Reviewed; 385 AA.
AC Q6ZD95; A0A0P0XBV0;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Meiotic recombination protein SPO11-2;
DE Short=OsSPO11-2;
DE AltName: Full=OsSPO11B;
DE AltName: Full=Topoisomerase 6 subunit A2;
DE Short=OsTOP6A2;
GN Name=SPO11-2; OrderedLocusNames=Os08g0156900, LOC_Os08g06050;
GN ORFNames=P0438H08.23-1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RA Yamada K., Kawagishi-Kobayashi M., Shingu Y., Mikawa T., Wakasa K.,
RA Shibata T.;
RT "Isolation and characterization of rice SPO11 genes.";
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Required for meiotic recombination. Mediates DNA cleavage
CC that forms the double-strand breaks (DSB) that initiate meiotic
CC recombination (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q57815};
CC -!- SUBUNIT: Interacts with TOP6B. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TOP6A family. {ECO:0000305}.
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DR EMBL; AB219538; BAF65345.1; -; mRNA.
DR EMBL; AP004460; BAC99506.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF22941.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT03899.1; -; Genomic_DNA.
DR RefSeq; XP_015650817.1; XM_015795331.1.
DR AlphaFoldDB; Q6ZD95; -.
DR SMR; Q6ZD95; -.
DR STRING; 4530.OS08T0156900-01; -.
DR PaxDb; Q6ZD95; -.
DR PRIDE; Q6ZD95; -.
DR EnsemblPlants; Os08t0156900-01; Os08t0156900-01; Os08g0156900.
DR GeneID; 4344697; -.
DR Gramene; Os08t0156900-01; Os08t0156900-01; Os08g0156900.
DR KEGG; osa:4344697; -.
DR eggNOG; KOG2795; Eukaryota.
DR InParanoid; Q6ZD95; -.
DR OMA; CTRSLMR; -.
DR OrthoDB; 1272299at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR ExpressionAtlas; Q6ZD95; baseline and differential.
DR Genevisible; Q6ZD95; OS.
DR GO; GO:0000228; C:nuclear chromosome; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051026; P:chiasma assembly; IEA:EnsemblPlants.
DR GO; GO:0009553; P:embryo sac development; IEA:EnsemblPlants.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IBA:GO_Central.
DR GO; GO:0000706; P:meiotic DNA double-strand break processing; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IEA:EnsemblPlants.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
DR GO; GO:0048316; P:seed development; IEA:EnsemblPlants.
DR CDD; cd00223; TOPRIM_TopoIIB_SPO; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR002815; Spo11/TopoVI_A.
DR InterPro; IPR013049; Spo11/TopoVI_A_N.
DR InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR InterPro; IPR034136; TOPRIM_Topo6A/Spo11.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR10848; PTHR10848; 1.
DR Pfam; PF04406; TP6A_N; 1.
DR PRINTS; PR01550; TOP6AFAMILY.
DR SUPFAM; SSF56726; SSF56726; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Metal-binding; Nucleus; Reference proteome.
FT CHAIN 1..385
FT /note="Meiotic recombination protein SPO11-2"
FT /id="PRO_0000429778"
FT ACT_SITE 126
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q9M4A2"
FT BINDING 219
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q57815"
FT BINDING 272
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q57815"
SQ SEQUENCE 385 AA; 42741 MW; 6CA4B0E609F3BB1E CRC64;
MAEAGVAAAS LFGADRRLCS ADILPPAEVR ARIEVAVLNF LAALTDPAAP AISALPLISR
GAANRGLRRA LLRDDVSSVY LSYASCKRSL TRANDAKAFV RVWKVMEMCY KILGEGKLVT
LRELFYTLLS ESPTYFTCQR HVNQTVQDVV SLLRCTRQSL GIMASSRGAL IGRLVVQGPE
EEHVDCSILG PSGHAITGDL NVLSKLIFSS DARYIIVVEK DAIFQRLAED RIYSHLPCIL
ITAKGYPDLA TRFILHRLSQ TYPNMPIFAL VDWNPAGLAI LCTYKYGSIS MGLESYRYAC
NVKWLGLRGD DLQLIPQSAY QELKPRDLQI AKSLLSSKFL QDKHRAELTL MLETGKRAEI
EALYSHGFDF LGKYVARKIV QGDYI
