SPO12_YEAST
ID SPO12_YEAST Reviewed; 173 AA.
AC P17123; D3DLA1;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Sporulation-specific protein 12;
GN Name=SPO12; OrderedLocusNames=YHR152W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2188099; DOI=10.1128/mcb.10.6.2809-2819.1990;
RA Malavasic M.J., Elder R.T.;
RT "Complementary transcripts from two genes necessary for normal meiosis in
RT the yeast Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 10:2809-2819(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION AS A COMPONENT OF THE FEAR NETWORK.
RX PubMed=11832211; DOI=10.1016/s0092-8674(02)00618-9;
RA Stegmeier F., Visintin R., Amon A.;
RT "Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function
RT in a network that controls Cdc14 localization during early anaphase.";
RL Cell 108:207-220(2002).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-118 AND SER-125, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: It is required for meiosis I chromosome division during
CC sporulation. A component of the FEAR (CDC14 early anaphase release)
CC network which promotes CDC14 release from the nucleolus during early
CC anaphase. {ECO:0000269|PubMed:11832211}.
CC -!- MISCELLANEOUS: Its negative tail is functionally important.
CC -!- MISCELLANEOUS: Present with 937 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; M32653; AAA35076.1; -; Genomic_DNA.
DR EMBL; U10397; AAB68979.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06845.1; -; Genomic_DNA.
DR PIR; B36321; B36321.
DR RefSeq; NP_012022.1; NM_001179283.1.
DR AlphaFoldDB; P17123; -.
DR BioGRID; 36586; 177.
DR DIP; DIP-1654N; -.
DR IntAct; P17123; 36.
DR MINT; P17123; -.
DR STRING; 4932.YHR152W; -.
DR iPTMnet; P17123; -.
DR PaxDb; P17123; -.
DR PRIDE; P17123; -.
DR EnsemblFungi; YHR152W_mRNA; YHR152W; YHR152W.
DR GeneID; 856557; -.
DR KEGG; sce:YHR152W; -.
DR SGD; S000001195; SPO12.
DR VEuPathDB; FungiDB:YHR152W; -.
DR eggNOG; ENOG502SAUW; Eukaryota.
DR HOGENOM; CLU_1548496_0_0_1; -.
DR InParanoid; P17123; -.
DR BioCyc; YEAST:G3O-31187-MON; -.
DR PRO; PR:P17123; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P17123; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0007127; P:meiosis I; IMP:SGD.
DR GO; GO:1904750; P:negative regulation of protein localization to nucleolus; IMP:SGD.
DR GO; GO:0031536; P:positive regulation of exit from mitosis; IMP:SGD.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR007727; Spo12.
DR Pfam; PF05032; Spo12; 1.
PE 1: Evidence at protein level;
KW Acetylation; Meiosis; Phosphoprotein; Reference proteome; Sporulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..173
FT /note="Sporulation-specific protein 12"
FT /id="PRO_0000072133"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 159..173
FT /note="Negative-charged tail"
FT COMPBIAS 1..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 118
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 125
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 173 AA; 19912 MW; E114394516451F60 CRC64;
MSNKASDQSA RTASILKTDI TRENTITRSS SSNNDNYHHH NNINNYNESA KTGEDANKEN
IPNLEEEIAA FRIFRKKSTS NLKSSHTTSN LVKKTMFKRD LLKQDPKRKL QLQQRFASPT
DRLVSPCSLK LNEHKVKMFG KKKKVNPMKL NFKGNLAADS EDVEIDEDEE YFY
