SPO16_MOUSE
ID SPO16_MOUSE Reviewed; 186 AA.
AC Q3KQP7; G3X9D1; Q8CF32;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2021, sequence version 3.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Protein SPO16 homolog {ECO:0000303|PubMed:30746471};
DE AltName: Full=Synaptonemal complex reinforcing element {ECO:0000303|PubMed:30949703};
GN Name=Spo16 {ECO:0000303|PubMed:30746471};
GN Synonyms=Scre {ECO:0000303|PubMed:30949703};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH SHOC1,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RC TISSUE=Testis;
RX PubMed=30746471; DOI=10.1126/sciadv.aau9780;
RA Zhang Q., Ji S.Y., Busayavalasa K., Yu C.;
RT "SPO16 binds SHOC1 to promote homologous recombination and crossing-over in
RT meiotic prophase I.";
RL Sci. Adv. 5:eaau9780-eaau9780(2019).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, INTERACTION WITH SYCP1 AND SYCE3, SUBUNIT, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=30949703; DOI=10.1093/nar/gkz226;
RA Liu H., Huang T., Li M., Li M., Zhang C., Jiang J., Yu X., Yin Y.,
RA Zhang F., Lu G., Luo M.C., Zhang L.R., Li J., Liu K., Chen Z.J.;
RT "SCRE serves as a unique synaptonemal complex fastener and is essential for
RT progression of meiosis prophase I in mice.";
RL Nucleic Acids Res. 47:5670-5683(2019).
CC -!- FUNCTION: Plays a key role in reinforcing the integrity of the central
CC element of the synaptonemal complex (SC) thereby stabilizing SC,
CC ensuring progression of meiotic prophase I in male and female germ
CC cells (PubMed:30949703). Promotes homologous recombination and
CC crossing-over in meiotic prophase I via its association with SHOC1
CC (PubMed:30746471). Required for the localization of TEX11 and MSH4 to
CC recombination intermediates (PubMed:30746471).
CC {ECO:0000269|PubMed:30746471, ECO:0000269|PubMed:30949703}.
CC -!- SUBUNIT: Homooligomer (PubMed:30949703). Interacts with SHOC1
CC (PubMed:30746471). Interacts with SYCP1 and SYCE3 (PubMed:30949703).
CC {ECO:0000269|PubMed:30746471, ECO:0000269|PubMed:30949703}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:30746471,
CC ECO:0000269|PubMed:30949703}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3KQP7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3KQP7-2; Sequence=VSP_027516, VSP_027517;
CC -!- TISSUE SPECIFICITY: Testis (at protein level).
CC {ECO:0000269|PubMed:30746471, ECO:0000269|PubMed:30949703}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryonic ovaries at embryonic day
CC 16.5 (at protein level). {ECO:0000269|PubMed:30746471,
CC ECO:0000269|PubMed:30949703}.
CC -!- DISRUPTION PHENOTYPE: Mice show a massive germline loss and infertility
CC in both males and females (PubMed:30746471, PubMed:30949703). Defects
CC in meiotic prophase progression and meiotic recombination seen in
CC spermatocytes and oocytes (PubMed:30746471). Meiocytes fail to achieve
CC complete synapsis and show repair of double-strand breaks without
CC formation of crossovers (PubMed:30746471).
CC {ECO:0000269|PubMed:30746471, ECO:0000269|PubMed:30949703}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25144.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK006463; BAC25144.1; ALT_FRAME; mRNA.
DR EMBL; AC134411; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC106106; AAI06107.1; -; mRNA.
DR RefSeq; NP_877422.1; NM_182745.2. [Q3KQP7-1]
DR AlphaFoldDB; Q3KQP7; -.
DR SMR; Q3KQP7; -.
DR STRING; 10090.ENSMUSP00000058373; -.
DR PhosphoSitePlus; Q3KQP7; -.
DR PaxDb; Q3KQP7; -.
DR PRIDE; Q3KQP7; -.
DR Antibodypedia; 53285; 50 antibodies from 11 providers.
DR Ensembl; ENSMUST00000239050; ENSMUSP00000159004; ENSMUSG00000089798. [Q3KQP7-1]
DR GeneID; 76421; -.
DR KEGG; mmu:76421; -.
DR UCSC; uc008ymk.1; mouse.
DR MGI; MGI:1923671; 1700028K03Rik.
DR VEuPathDB; HostDB:ENSMUSG00000089798; -.
DR VEuPathDB; HostDB:ENSMUSG00000106631; -.
DR eggNOG; ENOG502S1KQ; Eukaryota.
DR GeneTree; ENSGT00390000008285; -.
DR InParanoid; Q3KQP7; -.
DR OrthoDB; 1325546at2759; -.
DR PhylomeDB; Q3KQP7; -.
DR TreeFam; TF333385; -.
DR BioGRID-ORCS; 76421; 1 hit in 72 CRISPR screens.
DR ChiTaRS; 1700028K03Rik; mouse.
DR PRO; PR:Q3KQP7; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q3KQP7; protein.
DR Bgee; ENSMUSG00000089798; Expressed in spermatocyte and 30 other tissues.
DR ExpressionAtlas; Q3KQP7; baseline and differential.
DR GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR GO; GO:0007130; P:synaptonemal complex assembly; IMP:UniProtKB.
DR InterPro; IPR027857; SCRE.
DR PANTHER; PTHR31408; PTHR31408; 1.
DR Pfam; PF15162; SCRE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; Meiosis; Reference proteome.
FT CHAIN 1..186
FT /note="Protein SPO16 homolog"
FT /id="PRO_0000299021"
FT VAR_SEQ 140
FT /note="T -> R (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027516"
FT VAR_SEQ 141..186
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_027517"
SQ SEQUENCE 186 AA; 21131 MW; 63504DFCD6C792F5 CRC64;
MAMDERRGKE RVQWTTTIII SSSLKSYEIA TALENRSHKV RYSDTLESGS IVFSLSGVAF
LLMDAKECMT SAEEIFVTKI EKFINIHQNS FLVLFAPLHG PEEWSLMFRI HQRFLGSNLR
ILPVHNTVNA LDLMCTIAKT TSKPHIDSIC YRMITTKAYI IEQSPVWRTL QKIKLSSDSV
SADSGE
