SPO19_YEAST
ID SPO19_YEAST Reviewed; 223 AA.
AC Q03029; D6W3N7;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Sporulation-specific protein 19;
DE Flags: Precursor;
GN Name=SPO19; OrderedLocusNames=YPL130W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=10383953; DOI=10.1128/jb.181.13.3886-3889.1999;
RA Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K.;
RT "Amino acid residues in the omega-minus region participate in cellular
RT localization of yeast glycosylphosphatidylinositol-attached proteins.";
RL J. Bacteriol. 181:3886-3889(1999).
RN [5]
RP FUNCTION.
RX PubMed=11101837; DOI=10.1038/82539;
RA Primig M., Williams R.M., Winzeler E.A., Tevzadze G.G., Conway A.R.,
RA Hwang S.Y., Davis R.W., Esposito R.E.;
RT "The core meiotic transcriptome in budding yeasts.";
RL Nat. Genet. 26:415-423(2000).
CC -!- FUNCTION: Involved in sporulation. Essential for completion of the
CC nuclear division. {ECO:0000269|PubMed:11101837}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000305|PubMed:10383953}. Membrane {ECO:0000305}; Lipid-anchor,
CC GPI-anchor {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in late pachytene.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By
CC similarity). {ECO:0000250}.
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DR EMBL; U43703; AAB68229.1; -; Genomic_DNA.
DR EMBL; AY693111; AAT93130.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11303.1; -; Genomic_DNA.
DR PIR; S69054; S69054.
DR RefSeq; NP_015195.1; NM_001183944.1.
DR AlphaFoldDB; Q03029; -.
DR SMR; Q03029; -.
DR BioGRID; 36051; 124.
DR DIP; DIP-3997N; -.
DR IntAct; Q03029; 2.
DR MINT; Q03029; -.
DR STRING; 4932.YPL130W; -.
DR PaxDb; Q03029; -.
DR PRIDE; Q03029; -.
DR EnsemblFungi; YPL130W_mRNA; YPL130W; YPL130W.
DR GeneID; 855973; -.
DR KEGG; sce:YPL130W; -.
DR SGD; S000006051; SPO19.
DR VEuPathDB; FungiDB:YPL130W; -.
DR HOGENOM; CLU_1240746_0_0_1; -.
DR InParanoid; Q03029; -.
DR OMA; FIDFNEY; -.
DR BioCyc; YEAST:G3O-34029-MON; -.
DR PRO; PR:Q03029; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q03029; protein.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR GO; GO:0005628; C:prospore membrane; IDA:SGD.
DR GO; GO:0051321; P:meiotic cell cycle; IGI:SGD.
DR GO; GO:0070583; P:spore membrane bending pathway; IMP:SGD.
PE 2: Evidence at transcript level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Meiosis; Membrane;
KW Reference proteome; Secreted; Signal; Sporulation.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..198
FT /note="Sporulation-specific protein 19"
FT /id="PRO_0000019590"
FT PROPEP 199..223
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000372457"
FT LIPID 198
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 223 AA; 24989 MW; 337E73F322515939 CRC64;
MKKQILIVAA QSILCSTVFG ERSNVGLSTE ELGGDSILYF NEDPIVIEID KKAIDKKTLE
QLASTRDVVL TDLPDTLEFI DFNEYAKMKS KSDMLLEYIN EYEFDDFERS SEGGLEEEEE
EDLIYDFNAQ AEDLGKLGSN IYEVVEEKNI VNTYDGNLIN ASTTESTTTI RPFVTSHSYV
ASSTPYSNIS SLNEDYDNAS NFLTPTTVAL AVLLTILLFI QAY
